ALG5/YPL227C Protein Information

Standard Name	Alg5p
Systematic Name	Ypl227cp
ORF Classification	Verified
Description	UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (1)
Name Description	Asparagine-Linked Glycosylation 2

Experimental Data
Molecules/cell	5800 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	334
Molecular Weight (Da)	38,346
Isoelectric Point (pI)	9.17

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Alg5p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 35 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012583B \| P40350 MIPS: YPL227C NCBI: 1181261 \| 1370470 \| 535141 \| 6325029 \| 728823 \| NP_015097.1 GenBank/EMBL/DDBJ: DAA11209.1 \| X77573 \| X94561 \| Z73583
External Classifications	EC: 2.4.1.117 [Dolichyl-phosphate beta-glucosyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MRALRFLIEN RNTVFFTLLV ALVLSLYLLV YLFSHTPRPP YPEELKYIAI 51 DEKGHEVSRA LPNLNEHQDD EEIFLSVVIP SYNETGRILL MLTDAISFLK 101 EKYGSRWEIV IVDDGSTDNT TQYCLKICKE QFKLNYEQFR IIKFSQNRGK 151 GGAVRQGFLH IRGKYGLFAD ADGASKFSDV EKLIDAISKI ETSSTDLKTT 201 KPAVAIGSRA HMVNTEAVIK RSMIRNCLMY GFHTLVFIFG IRSIKDTQCG 251 FKLFNRAAIL KIFPYLHTEG WIFDVEILIL AIRKRIQIEE IPISWHEVDG 301 SKMALAIDSI KMAKDLVIIR MAYLLGIYRD NKKC*

Amino Acid Sequence (or in FASTA format)

       1  MRALRFLIEN RNTVFFTLLV ALVLSLYLLV YLFSHTPRPP YPEELKYIAI
      51  DEKGHEVSRA LPNLNEHQDD EEIFLSVVIP SYNETGRILL MLTDAISFLK
     101  EKYGSRWEIV IVDDGSTDNT TQYCLKICKE QFKLNYEQFR IIKFSQNRGK
     151  GGAVRQGFLH IRGKYGLFAD ADGASKFSDV EKLIDAISKI ETSSTDLKTT
     201  KPAVAIGSRA HMVNTEAVIK RSMIRNCLMY GFHTLVFIFG IRSIKDTQCG
     251  FKLFNRAAIL KIFPYLHTEG WIFDVEILIL AIRKRIQIEE IPISWHEVDG
     301  SKMALAIDSI KMAKDLVIIR MAYLLGIYRD NKKC*

external links for Alg5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Alg5p

1)	Heesen S, et al. (1994) Isolation of the ALG5 locus encoding the UDP-glucose:dolichyl-phosphate glucosyltransferase from Saccharomyces cerevisiae. Eur J Biochem 224(1):71-9
2)	Huffaker TC and Robbins PW (1982) Temperature-sensitive yeast mutants deficient in asparagine-linked glycosylation. J Biol Chem 257(6):3203-10
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41