SAR1/YPL218W Protein Information

Standard Name	Sar1p 1
Systematic Name	Ypl218wp
ORF Classification	Verified
Description	GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport; lowers membrane rigidity helping in vesicle formation (2, 3, 4)
Name Description	Secretion-Associated, Ras-related 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	190
Molecular Weight (Da)	21,450
Isoelectric Point (pI)	5.12

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sar1p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 56 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000036CA2 \| P20606 MIPS: YPL218W NCBI: 134221 \| 1370452 \| 165761045 \| 24158934 \| 24158936 \| 4422 \| 6325038 \| NP_015106.1 GenBank/EMBL/DDBJ: DAA11218.1 \| X51667 \| Z73574
External Classifications	EC: 3.6.5.- [Hydrolases, Acting on GTP; involved in cellular and subcellular movement]

Amino Acid Sequence (or in FASTA format)
1 MAGWDIFGWF RDVLASLGLW NKHGKLLFLG LDNAGKTTLL HMLKNDRLAT 51 LQPTWHPTSE ELAIGNIKFT TFDLGGHIQA RRLWKDYFPE VNGIVFLVDA 101 ADPERFDEAR VELDALFNIA ELKDVPFVIL GNKIDAPNAV SEAELRSALG 151 LLNTTGSQRI EGQRPVEVFM CSVVMRNGYL EAFQWLSQYI *

Amino Acid Sequence (or in FASTA format)

       1  MAGWDIFGWF RDVLASLGLW NKHGKLLFLG LDNAGKTTLL HMLKNDRLAT
      51  LQPTWHPTSE ELAIGNIKFT TFDLGGHIQA RRLWKDYFPE VNGIVFLVDA
     101  ADPERFDEAR VELDALFNIA ELKDVPFVIL GNKIDAPNAV SEAELRSALG
     151  LLNTTGSQRI EGQRPVEVFM CSVVMRNGYL EAFQWLSQYI *

external links for Sar1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Sar1p

1)	Nakano A and Muramatsu M (1989) A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus. J Cell Biol 109(6 Pt 1):2677-91
2)	Barlowe C, et al. (1993) Purification and characterization of SAR1p, a small GTP-binding protein required for transport vesicle formation from the endoplasmic reticulum. J Biol Chem 268(2):873-9
3)	Giraudo CG and Maccioni HJ (2003) Endoplasmic reticulum export of glycosyltransferases depends on interaction of a cytoplasmic dibasic motif with Sar1. Mol Biol Cell 14(9):3753-66
4)	Settles EI, et al. (2010) The vesicle trafficking protein Sar1 lowers lipid membrane rigidity. Biophys J 99(5):1539-45