DDC1/YPL194W Protein Information Help

Standard Name Ddc1p 1
Systematic Name Ypl194wp
ORF Classification Verified
Description DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress (2, 3, 4, 5)
Name Description DNA Damage Checkpoint 1
Experimental Data
Molecules/cell 238 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 612
Molecular Weight (Da) 69,693
Isoelectric Point (pI) 6.58

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ddc1p (InterPro)
Physical Interactions There are 33 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000069AED | Q08949
MIPS: YPL194W
NCBI: 1370405 | 6325062 | 74583770 | NP_015130.1
GenBank/EMBL/DDBJ: DAA11240.1 | Z73550
Amino Acid Sequence (or in FASTA format)
 
       1  MSFKATITES GKQNIWFRAI YVLSTIQDDI KITVTTNELI AWSMNETDTT
      51  LCQVRFQKSF FEEYEFKPHE IVFGENGVQV IEDTYGNSHK LYSFRVNGRH
     101  LTTISRKPDG DGIKSFTIAV NNTSTCPESL ANRLIVVIEM DSLIVKEYCP
     151  QFQPIKYDPI IINLKYKRRF LDVFGTAASD RNPQEPLDPK LLDVFTNTER
     201  ELTSALFNEE VESDIRKRNQ LTAADEINYI CCNSTLLKNF LDNCNVNVTD
     251  EVKLEINVHR LSITAFTKAV YGKNNDLLRN ALSMSNTIST LDLEHYCLFT
     301  TIEDEKQDKR SHSKRREHMK SIIFKLKDFK NFITIGPSWK TTQDGNDNIS
     351  LWFCHPGDPI LMQMQKPGVK LELVEVTDSN INDDILEGKF IKTAISGSKE
     401  EAGLKDNKES CESPLKSKTA LKRENLPHSV AGTRNSPLKV SYLTPDNGST
     451  VAKTYRNNTA RKLFVEEQSQ STNYEQDKRF RQASSVHMNM NREQSFDIGT
     501  THEVACPRNE SNSLKRSIAD ICNETEDPTQ QSTFAKRADT TVTWGKALPA
     551  ADDEVSCSNI DRKGMLKKEK LKHMQGLLNS QNDTSNHKKQ DNKEMEDGLG
     601  LTQVEKPRGI FD*

external links for Ddc1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome

YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ddc1p
1) Longhese MP, et al.  (1997) The novel DNA damage checkpoint protein ddc1p is phosphorylated periodically during the cell cycle and in response to DNA damage in budding yeast. EMBO J 16(17):5216-26
2) Hong EJ and Roeder GS  (2002) A role for Ddc1 in signaling meiotic double-strand breaks at the pachytene checkpoint. Genes Dev 16(3):363-76
3) Wang H and Elledge SJ  (2002) Genetic and physical interactions between DPB11 and DDC1 in the yeast DNA damage response pathway. Genetics 160(4):1295-304
4) Giannattasio M, et al.  (2003) Correlation between checkpoint activation and in vivo assembly of the yeast checkpoint complex Rad17-Mec3-Ddc1. J Biol Chem 278(25):22303-8
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41