TGS1/YPL157W Protein Information

Standard Name	Tgs1p 1
Systematic Name	Ypl157wp
ORF Classification	Verified
Description	Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs (1, 2, 3, 4)
Name Description	TrimethylGuanosine Synthase 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	315
Molecular Weight (Da)	36,527
Isoelectric Point (pI)	10.28

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tgs1p (InterPro)
Physical Interactions	There are 8 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000069B88 \| Q12052 MIPS: YPL157W NCBI: 1370334 \| 1403552 \| 6325100 \| 74583797 \| NP_015168.1 GenBank/EMBL/DDBJ: DAA11277.1 \| X96770 \| Z73513
External Classifications	EC: 2.1.1.- [Transferases transferring one-carbon groups methyltransferases]

Amino Acid Sequence (or in FASTA format)
1 MGRTFIHASK IKHAARKRKH HSNFRTLIKL LNNDAYKIES SKPLKNGKLF 51 KYWKNRRRLF SKIDSASIYM TDELWFSVTP ERIACFLANF VKACMPNAER 101 ILDVFCGGGG NTIQFAMQFP YVYGVDYSIE HIYCTAKNAQ SYGVDDRIWL 151 KRGSWKKLVS KQKLSKIKYD CVFGSPPWGG PEYLRNDVYD LEQHLKPMGI 201 TKMLKSFLKL SPNVIMFLPR NSDLNQLSRA TRKVLGPFAK CKVLYVKENG 251 YMKGIFCMWG ECFFNYEPAS TENSRRESSE KEELSSENEE LSKRKKHEST 301 TTTKDNTVDI YDVNG*

Amino Acid Sequence (or in FASTA format)

       1  MGRTFIHASK IKHAARKRKH HSNFRTLIKL LNNDAYKIES SKPLKNGKLF
      51  KYWKNRRRLF SKIDSASIYM TDELWFSVTP ERIACFLANF VKACMPNAER
     101  ILDVFCGGGG NTIQFAMQFP YVYGVDYSIE HIYCTAKNAQ SYGVDDRIWL
     151  KRGSWKKLVS KQKLSKIKYD CVFGSPPWGG PEYLRNDVYD LEQHLKPMGI
     201  TKMLKSFLKL SPNVIMFLPR NSDLNQLSRA TRKVLGPFAK CKVLYVKENG
     251  YMKGIFCMWG ECFFNYEPAS TENSRRESSE KEELSSENEE LSKRKKHEST
     301  TTTKDNTVDI YDVNG*

external links for Tgs1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Tgs1p

1)	Mouaikel J, et al. (2002) Hypermethylation of the cap structure of both yeast snRNAs and snoRNAs requires a conserved methyltransferase that is localized to the nucleolus. Mol Cell 9(4):891-901
2)	Colau G, et al. (2004) The small nucle(ol)ar RNA cap trimethyltransferase is required for ribosome synthesis and intact nucleolar morphology. Mol Cell Biol 24(18):7976-86
3)	Franke J, et al. (2008) Hypermethylation of yeast telomerase RNA by the snRNA and snoRNA methyltransferase Tgs1. J Cell Sci 121(Pt 21):3553-60
4)	Qiu ZR, et al. (2011) An essential role for trimethylguanosine RNA caps in Saccharomyces cerevisiae meiosis and their requirement for splicing of SAE3 and PCH2 meiotic pre-mRNAs. Nucleic Acids Res 39(13):5633-46