PEP4/YPL154C Protein Information

Standard Name	Pep4p 1, 2, 3, 4, 5, 6, 7
Systematic Name	Ypl154cp
Alias	Pho9p , Pra1p , Yscap
ORF Classification	Verified
Description	Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates (10, 11, 12, 13, 14, 8, 9)
Name Description	carboxyPEPtidase Y-deficient 15, 16

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	405
Molecular Weight (Da)	44,499
Isoelectric Point (pI)	4.54

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pep4p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 22 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000532A5 \| P07267 MIPS: YPL154C NCBI: 10835733 \| 10835734 \| 10835735 \| 10835736 \| 10835737 \| 115643 \| 1370328 \| 1403555 \| 14278413 \| 172122 \| 22218637 \| 22218638 \| 22218639 \| 2624629 \| 3853 \| 6325103 \| 7766834 \| 7766836 \| NP_015171.1 \| NM_001183968.1 GenBank/EMBL/DDBJ: DAA11280.1 \| M13358 \| X96770 \| Z11963 \| Z73510
External Classifications	EC: 3.4.23.25 [Saccharopepsin]

Amino Acid Sequence (or in FASTA format)
1 MFSLKALLPL ALLLVSANQV AAKVHKAKIY KHELSDEMKE VTFEQHLAHL 51 GQKYLTQFEK ANPEVVFSRE HPFFTEGGHD VPLTNYLNAQ YYTDITLGTP 101 PQNFKVILDT GSSNLWVPSN ECGSLACFLH SKYDHEASSS YKANGTEFAI 151 QYGTGSLEGY ISQDTLSIGD LTIPKQDFAE ATSEPGLTFA FGKFDGILGL 201 GYDTISVDKV VPPFYNAIQQ DLLDEKRFAF YLGDTSKDTE NGGEATFGGI 251 DESKFKGDIT WLPVRRKAYW EVKFEGIGLG DEYAELESHG AAIDTGTSLI 301 TLPSGLAEMI NAEIGAKKGW TGQYTLDCNT RDNLPDLIFN FNGYNFTIGP 351 YDYTLEVSGS CISAITPMDF PEPVGPLAIV GDAFLRKYYS IYDLGNNAVG 401 LAKAI*

Amino Acid Sequence (or in FASTA format)

       1  MFSLKALLPL ALLLVSANQV AAKVHKAKIY KHELSDEMKE VTFEQHLAHL
      51  GQKYLTQFEK ANPEVVFSRE HPFFTEGGHD VPLTNYLNAQ YYTDITLGTP
     101  PQNFKVILDT GSSNLWVPSN ECGSLACFLH SKYDHEASSS YKANGTEFAI
     151  QYGTGSLEGY ISQDTLSIGD LTIPKQDFAE ATSEPGLTFA FGKFDGILGL
     201  GYDTISVDKV VPPFYNAIQQ DLLDEKRFAF YLGDTSKDTE NGGEATFGGI
     251  DESKFKGDIT WLPVRRKAYW EVKFEGIGLG DEYAELESHG AAIDTGTSLI
     301  TLPSGLAEMI NAEIGAKKGW TGQYTLDCNT RDNLPDLIFN FNGYNFTIGP
     351  YDYTLEVSGS CISAITPMDF PEPVGPLAIV GDAFLRKYYS IYDLGNNAVG
     401  LAKAI*

external links for Pep4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Pep4p

1)	Fassler, J. (1992) Personal Communication, Mortimer Map Edition 11
2)	Jones, E. (1985) Personal Communication, Mortimer Map Edition 9
3)	Petitjean, A. and Tatchell, K. (1989) Personal Communication, Mortimer Map Edition 10
4)	Schweizer, E. (1989) Personal Communication, Mortimer Map Edition 10
5)	Stearns, T. and Botstein, D. (1989) Personal Communication, Mortimer Map Edition 10
6)	Sumrada, R. and Cooper, T. (1992) Personal Communication, Mortimer Map Edition 11
7)	Yoshida, K. (1989) Personal Communication, Mortimer Map Edition 10
8)	Rupp S and Wolf DH (1995) Biogenesis of the yeast vacuole (lysosome). The use of active-site mutants of proteinase yscA to determine the necessity of the enzyme for vacuolar proteinase maturation and proteinase yscB stability. Eur J Biochem 231(1):115-25
9)	van den Hazel HB, et al. (1993) The propeptide is required for in vivo formation of stable active yeast proteinase A and can function even when not covalently linked to the mature region. J Biol Chem 268(24):18002-7
10)	van den Hazel HB, et al. (1992) Autoactivation of proteinase A initiates activation of yeast vacuolar zymogens. Eur J Biochem 207(1):277-83
11)	Wolff AM, et al. (1996) Vacuolar and extracellular maturation of Saccharomyces cerevisiae proteinase A. Yeast 12(9):823-32
12)	Ammerer G, et al. (1986) PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors. Mol Cell Biol 6(7):2490-9
13)	Woolford CA, et al. (1986) The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases. Mol Cell Biol 6(7):2500-10
14)	Marques M, et al. (2006) The Pep4p vacuolar proteinase contributes to the turnover of oxidized proteins but PEP4 overexpression is not sufficient to increase chronological lifespan in Saccharomyces cerevisiae. Microbiology 152(Pt 12):3595-605
15)	Jones EW, et al. (1982) PEP4 gene function is required for expression of several vacuolar hydrolases in Saccharomyces cerevisiae. Genetics 102(4):665-77
16)	Jones EW (1977) Proteinase mutants of Saccharomyces cerevisiae. Genetics 85(1):23-33