RAD53/YPL153C Protein Information Help

Standard Name Rad53p 1
Systematic Name Ypl153cp
Alias Lsd1p , Mec2p , Spk1p
ORF Classification Verified
Description Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication (2, 3, 4)
Name Description RADiation sensitive
Experimental Data
Molecules/cell 6900 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 821
Molecular Weight (Da) 91,962
Isoelectric Point (pI) 7.82

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rad53p (InterPro)
Physical Interactions There are 236 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000005328E | P22216
MIPS: YPL153C
NCBI: 12084487 | 12084488 | 134835 | 1370326 | 1403556 | 159162211 | 159162303 | 159162304 | 159162532 | 159162534 | 159162574 | 159162576 | 159162912 | 172657 | 192987129 | 192987130 | 192987135 | 253722226 | 253722227 | 253722250 | 253722251 | 253722480 | 253722860 | 253722914 | 253722942 | 375332303 | 375332304 | 375332305 | 375332306 | 51013469 | 6325104 | NP_015172.1
GenBank/EMBL/DDBJ: DAA11281.1 | AY693009 | M55623 | X96770 | Z73509
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
EC: 2.7.12.1 [Dual-specificity kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MENITQPTQQ STQATQRFLI EKFSQEQIGE NIVCRVICTT GQIPIRDLSA
      51  DISQVLKEKR SIKKVWTFGR NPACDYHLGN ISRLSNKHFQ ILLGEDGNLL
     101  LNDISTNGTW LNGQKVEKNS NQLLSQGDEI TVGVGVESDI LSLVIFINDK
     151  FKQCLEQNKV DRIRSNLKNT SKIASPGLTS STASSMVANK TGIFKDFSII
     201  DEVVGQGAFA TVKKAIERTT GKTFAVKIIS KRKVIGNMDG VTRELEVLQK
     251  LNHPRIVRLK GFYEDTESYY MVMEFVSGGD LMDFVAAHGA VGEDAGREIS
     301  RQILTAIKYI HSMGISHRDL KPDNILIEQD DPVLVKITDF GLAKVQGNGS
     351  FMKTFCGTLA YVAPEVIRGK DTSVSPDEYE ERNEYSSLVD MWSMGCLVYV
     401  ILTGHLPFSG STQDQLYKQI GRGSYHEGPL KDFRISEEAR DFIDSLLQVD
     451  PNNRSTAAKA LNHPWIKMSP LGSQSYGDFS QISLSQSLSQ QKLLENMDDA
     501  QYEFVKAQRK LQMEQQLQEQ DQEDQDGKIQ GFKIPAHAPI RYTQPKSIEA
     551  ETREQKLLHS NNTENVKSSK KKGNGRFLTL KPLPDSIIQE SLEIQQGVNP
     601  FFIGRSEDCN CKIEDNRLSR VHCFIFKKRH AVGKSMYESP AQGLDDIWYC
     651  HTGTNVSYLN NNRMIQGTKF LLQDGDEIKI IWDKNNKFVI GFKVEINDTT
     701  GLFNEGLGML QEQRVVLKQT AEEKDLVKKL TQMMAAQRAN QPSASSSSMS
     751  AKKPPVSDTN NNGNNSVLND LVESPINANT GNILKRIHSV SLSQSQIDPS
     801  KKVKRAKLDQ TSKGPENLQF S*

external links for Rad53p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rad53p
1) Sitney, K.  (1989) Personal Communication, Mortimer Map Edition 10
2) Schwartz MF, et al.  (2002) Rad9 phosphorylation sites couple Rad53 to the Saccharomyces cerevisiae DNA damage checkpoint. Mol Cell 9(5):1055-65
3) Toh GW and Lowndes NF  (2003) Role of the Saccharomyces cerevisiae Rad9 protein in sensing and responding to DNA damage. Biochem Soc Trans 31(Pt 1):242-6
4) Dohrmann PR and Sclafani RA  (2006) Novel role for checkpoint Rad53 protein kinase in the initiation of chromosomal DNA replication in Saccharomyces cerevisiae. Genetics 174(1):87-99
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41