PRP46/YPL151C Protein Information

Standard Name	Prp46p 1
Systematic Name	Ypl151cp
Alias	Ntc50p 2
ORF Classification	Verified
Description	Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs (1, 2, 3, 4)
Name Description	Pre-mRNA Processing 1

Experimental Data
Molecules/cell	1770 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	451
Molecular Weight (Da)	50,700
Isoelectric Point (pI)	7.25

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Prp46p (InterPro)
Physical Interactions	There are 121 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FB5 \| Q12417 MIPS: YPL151C NCBI: 1370322 \| 1403558 \| 3122636 \| 6325106 \| NP_015174.1 GenBank/EMBL/DDBJ: DAA11284.1 \| X96770 \| Z73507

Amino Acid Sequence (or in FASTA format)
1 MDGNDHKVEN LGDVDKFYSR IRWNNQFSYM ATLPPHLQSE MEGQKSLLMR 51 YDTYRKESSS FSGEGKKVTL QHVPTDFSEA SQAVISKKDH DTHASAFVNK 101 IFQPEVAEEL IVNRYEKLLS QRPEWHAPWK LSRVINGHLG WVRCVAIDPV 151 DNEWFITGSN DTTMKVWDLA TGKLKTTLAG HVMTVRDVAV SDRHPYLFSV 201 SEDKTVKCWD LEKNQIIRDY YGHLSGVRTV SIHPTLDLIA TAGRDSVIKL 251 WDMRTRIPVI TLVGHKGPIN QVQCTPVDPQ VVSSSTDATV RLWDVVAGKT 301 MKVLTHHKRS VRATALHPKE FSVASACTDD IRSWGLAEGS LLTNFESEKT 351 GIINTLSINQ DDVLFAGGDN GVLSFYDYKS GHKYQSLATR EMVGSLEGER 401 SVLCSTFDKT GLRLITGEAD KSIKIWKQDE TATKESEPGL AWNPNLSAKR 451 F*

Amino Acid Sequence (or in FASTA format)

       1  MDGNDHKVEN LGDVDKFYSR IRWNNQFSYM ATLPPHLQSE MEGQKSLLMR
      51  YDTYRKESSS FSGEGKKVTL QHVPTDFSEA SQAVISKKDH DTHASAFVNK
     101  IFQPEVAEEL IVNRYEKLLS QRPEWHAPWK LSRVINGHLG WVRCVAIDPV
     151  DNEWFITGSN DTTMKVWDLA TGKLKTTLAG HVMTVRDVAV SDRHPYLFSV
     201  SEDKTVKCWD LEKNQIIRDY YGHLSGVRTV SIHPTLDLIA TAGRDSVIKL
     251  WDMRTRIPVI TLVGHKGPIN QVQCTPVDPQ VVSSSTDATV RLWDVVAGKT
     301  MKVLTHHKRS VRATALHPKE FSVASACTDD IRSWGLAEGS LLTNFESEKT
     351  GIINTLSINQ DDVLFAGGDN GVLSFYDYKS GHKYQSLATR EMVGSLEGER
     401  SVLCSTFDKT GLRLITGEAD KSIKIWKQDE TATKESEPGL AWNPNLSAKR
     451  F*

external links for Prp46p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Prp46p

1)	Albers M, et al. (2003) Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors. RNA 9(1):138-50
2)	Ohi MD and Gould KL (2002) Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. RNA 8(6):798-815
3)	Ohi MD, et al. (2002) Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol Cell Biol 22(7):2011-24
4)	Tardiff DF and Rosbash M (2006) Arrested yeast splicing complexes indicate stepwise snRNP recruitment during in vivo spliceosome assembly. RNA 12(6):968-79
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41