UME1/YPL139C Protein Information

Standard Name	Ume1p 1
Systematic Name	Ypl139cp
Alias	Wtm3p 2
ORF Classification	Verified
Description	Negative regulator of meiosis; required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p; UME1 has a paralog, WTM2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	Unscheduled Meiotic gene Expression 1

Experimental Data
Molecules/cell	3040 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	460
Molecular Weight (Da)	51,021
Isoelectric Point (pI)	5

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ume1p (InterPro)
Physical Interactions	There are 100 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053140 \| Q03010 MIPS: YPL139C NCBI: 1244776 \| 1781365 \| 2494904 \| 6325118 \| NP_015186.1 GenBank/EMBL/DDBJ: DAA11295.1 \| U10280 \| U43703

Amino Acid Sequence (or in FASTA format)
1 MSTLDIAEDN KIKNEEFKIW KKSIPSLYQH ISSLKPIFGS GVDESPSTLR 51 SIVFTNDSSC NKSKGVLSVP LLYSQGSEIF EVDCIVPLGL HYKKPESISE 101 PLVQPDYTME SQKVEQTVLI PKWEFKGETI AKMIYVDNSE INVKVIALST 151 NGSLAWFREG VKSPVYTMME PSTSLSSASS GNQNKPCVDF AISNDSKTLT 201 VTKEKHLDNE NATIKLIDNS GKIGEVLRTI PVPGIKNIQE IKFLNNQIFA 251 TCSDDGIIRF WGNEIGKKPL WILNDSLDGK TTCFAASPFV DTLFMTGTSG 301 GALKVWDIRA VIALGDADAE LNINQGHNKV NELFKVHHFY SEQVSKIEFS 351 SISPMEVVTI GGLGNVYHWN FEPVFAIYNE IHEDFQGIIS DELEAESMAF 401 YHTEGCRREI GENNKVNTVA YHKYIEDLVA TVDSDGLLTV YKPFTGKVLD 451 GSREVGAAKS *

Amino Acid Sequence (or in FASTA format)

       1  MSTLDIAEDN KIKNEEFKIW KKSIPSLYQH ISSLKPIFGS GVDESPSTLR
      51  SIVFTNDSSC NKSKGVLSVP LLYSQGSEIF EVDCIVPLGL HYKKPESISE
     101  PLVQPDYTME SQKVEQTVLI PKWEFKGETI AKMIYVDNSE INVKVIALST
     151  NGSLAWFREG VKSPVYTMME PSTSLSSASS GNQNKPCVDF AISNDSKTLT
     201  VTKEKHLDNE NATIKLIDNS GKIGEVLRTI PVPGIKNIQE IKFLNNQIFA
     251  TCSDDGIIRF WGNEIGKKPL WILNDSLDGK TTCFAASPFV DTLFMTGTSG
     301  GALKVWDIRA VIALGDADAE LNINQGHNKV NELFKVHHFY SEQVSKIEFS
     351  SISPMEVVTI GGLGNVYHWN FEPVFAIYNE IHEDFQGIIS DELEAESMAF
     401  YHTEGCRREI GENNKVNTVA YHKYIEDLVA TVDSDGLLTV YKPFTGKVLD
     451  GSREVGAAKS *

external links for Ume1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ume1p

1)	Strich R, et al. (1989) Identification of negative regulatory genes that govern the expression of early meiotic genes in yeast. Proc Natl Acad Sci U S A 86(24):10018-22
2)	Pemberton LF and Blobel G (1997) Characterization of the Wtm proteins, a novel family of Saccharomyces cerevisiae transcriptional modulators with roles in meiotic regulation and silencing. Mol Cell Biol 17(8):4830-41
3)	Kurdistani SK, et al. (2002) Genome-wide binding map of the histone deacetylase Rpd3 in yeast. Nat Genet 31(3):248-54
4)	Mallory MJ and Strich R (2003) Ume1p represses meiotic gene transcription in Saccharomyces cerevisiae through interaction with the histone deacetylase Rpd3p. J Biol Chem 278(45):44727-34
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41