SPP1/YPL138C Protein Information Help

Standard Name Spp1p 1
Systematic Name Ypl138cp
Alias Cps40p 2 , Saf41p 3
ORF Classification Verified
Description Subunit of COMPASS (Set1C); a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; relocalizes to the cytosol in response to hypoxia (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Set1c, Phd finger Protein
Experimental Data
Molecules/cell 1680 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 353
Molecular Weight (Da) 41,468
Isoelectric Point (pI) 6.73

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Spp1p (InterPro)
Physical Interactions There are 104 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052FF7 | Q03012
MIPS: YPL138C
NCBI: 1244777 | 61216382 | 6325119 | NP_015187.1 | NM_001183952.1
GenBank/EMBL/DDBJ: AAB68222.1 | DAA11296.1 | U43703
Amino Acid Sequence (or in FASTA format)
       1  MSLPQWCPPH STLKRNPTTG EDVYCICKRP DYGELMVGCD GCDDWFHFTC
      51  LHIPEQFKDL VFSFYCPYCQ AGITGKNKDA IINGEGSLPK TLWKRKCRIS
     101  DCYKPCLQDS KYCSEEHGRE FVNDIWSRLK TDEDRAVVKK MVEQTGHIDK
     151  FKKFGQLDFI DNNIVVKTDD EKEIFDQIVV RDMTLKTLED DLQEVQEISL
     201  PLFKKKLELL EVYLGWLDNV YTEMRKLDDD AASHVECGKE DSKGTKRKKK
     251  KNSSRSRARK NICGYCSTYE RIPCSVEEFV RDFGSNEEAT KIHEVCTKWK
     301  CNRHLDWVST NQEQYLQQID SLESMQERLQ HLIQARKKQL NIQYYEEILR
     351  RGL*                                                  

external links for Spp1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) Organelle DB
Candida (CGD) CYC2008 (complexes) MIPS YPL+
YGOB Complexome Pfam domains YeastGFP
YOGY DIP YeastRC Structure Prediction (Seattle) YeastRC Public Image Repository

GeneMANIA


IMP


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Spp1p
1) Roguev A, et al.  (2001) The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4. EMBO J 20(24):7137-48
2) Miller T, et al.  (2001) COMPASS: a complex of proteins associated with a trithorax-related SET domain protein. Proc Natl Acad Sci U S A 98(23):12902-7
3) Nagy PL, et al.  (2002) A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3. Proc Natl Acad Sci U S A 99(1):90-4
4) Krogan NJ, et al.  (2002) COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression. J Biol Chem 277(13):10753-5
5) Dover J, et al.  (2002) Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6. J Biol Chem 277(32):28368-71
6) Cheng H, et al.  (2004) The essential WD repeat protein Swd2 has dual functions in RNA polymerase II transcription termination and lysine 4 methylation of histone H3. Mol Cell Biol 24(7):2932-43
7) Kan J, et al.  (2008) Origin Recognition Complex (ORC) Mediates Histone 3 Lysine 4 Methylation through Cooperation with Spp1 in Saccharomyces cerevisiae. J Biol Chem 283(49):33803-7
8) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41