BEM3/YPL115C Protein Information Help

Standard Name Bem3p 1, 2
Systematic Name Ypl115cp
ORF Classification Verified
Description Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly (1)
Name Description Bud EMergence 3
Experimental Data
Molecules/cell 752 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,128
Molecular Weight (Da) 124,912
Isoelectric Point (pI) 6.83

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Bem3p (InterPro)
Physical Interactions There are 27 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000126890 | P32873
MIPS: YPL115C
NCBI: 1163099 | 2828204 | 418063 | 6325142 | NP_015210.1
GenBank/EMBL/DDBJ: DAA11319.1 | L14558 | U43503
Amino Acid Sequence (or in FASTA format)
 
       1  MTDNLTTTHG GSTTLELLAQ YNDHRSKKDK SIEHIEKGTC SGKERNPSYD
      51  EIFTENIKLK LQVQEYETEI ESLEKVIDML QKNREASLEV VLEQVQNDSR
     101  DSYVNDQSFV LPPRSAERKA HIKSLNLPIP TLSPPLQQGS DVALETSVTP
     151  TVPQIGVTSN TSISRKHLQN MILNDEIEAN SSFSSPKIIN RSVSSPTKIH
     201  SEQLASPAAS VTYTTSRITI KSPNKGSKSP LQERLRSPQN PNRMTAVINN
     251  HLHSPLKAST SNNLDELTES KSQQLTNDAI QKNDRVYSSI TSSAYTTGTP
     301  TSAAKSPSSL LEVKEGENKA LGFSPASKEK LDDFTQLLDS SFGEEDLVNT
     351  DSKDPLSIKS TINESLPPPP APPTFFSPTS SGNIKNSTPL SSHLASPVIL
     401  NKKDDNFGAQ SAKNLKKPVL TSSLPNLSTK LSTTSQNASL PPNPPVESSS
     451  KQKQLGETAS IHSTNTLNTF SSTPQGSLKT LRRPHASSVS TVKSVAQSLK
     501  SDIPLFVQPE DFGTIQIEVL STLYRDNEDD LSILIAIIDR KSGKEMFKFS
     551  KSIHKVRELD VYMKSHVPDL PLPTLPDRQL FQTLSPTKVD TRKNILNQYY
     601  TSIFSVPEFP KNVGLKIAQF ISTDTVMTPP MMDDNVKDGS LLLRRPKTLT
     651  GNSTWRVRYG ILRDDVLQLF DKNQLTETIK LRQSSIELIP NLPEDRFGTR
     701  NGFLITEHKK SGLSTSTKYY ICTETSKERE LWLSAFSDYI DPSQSLSLSS
     751  SRNANDTDSA SHLSAGTHHS KFGNATISAT DTPSYVTDLT QEYNNNNNIS
     801  NSSNNIANSD GIDSNPSSHS NFLASSSGNA EEEKDSRRAK MRSLFPFKKL
     851  TGPASAMNHI GITISNDSDS PTSPDSIIKS PSKKLMEVSS SSNSSTGPHV
     901  STAIFGSSLE TCLRLSSHKY QNVYDLPSVV YRCLEYLYKN RGIQEEGIFR
     951  LSGSSTVIKT LQERFDKEYD VDLCRYNESI EAKDDEASPS LYIGVNTVSG
    1001  LLKLYLRKLP HLLFGDEQFL SFKRVVDENH NNPVQISLGF KELIESGLVP
    1051  HANLSLMYAL FELLVRINEN SKFNKMNLRN LCIVFSPTLN IPISMLQPFI
    1101  TDFACIFQGG EPVKEEEREK VDIHIPQV*

external links for Bem3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Bem3p
1) Zheng Y, et al.  (1994) Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3. J Biol Chem 269(4):2369-72
2) Zheng Y, et al.  (1993) Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain. J Biol Chem 268(33):24629-34
3) Bender A and Pringle JR  (1991) Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol 11(3):1295-305
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41