PNG1/YPL096W Protein Information

Standard Name	Png1p
Systematic Name	Ypl096wp
ORF Classification	Verified
Description	Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p (1, 2, 3)
Name Description	Peptide N-Glycanase 1

Experimental Data
Molecules/cell	4850 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	363
Molecular Weight (Da)	42,485
Isoelectric Point (pI)	6.6

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Png1p (InterPro)
Physical Interactions	There are 16 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006C380 \| Q02890 MIPS: YPL096W NCBI: 1151230 \| 212375046 \| 6325161 \| 71041937 \| 71041939 \| 74676322 \| NP_015229.1 GenBank/EMBL/DDBJ: DAA11337.1 \| U43281
External Classifications	EC: 3.5.1.52 [Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase]

Amino Acid Sequence (or in FASTA format)
1 MGEVYEKNNI DFDSIAKMLL IKYKDFILSK FKKAAPVENI RFQNLVHTNQ 51 FAQGVLGQSQ HLCTVYDNPS WHSIVLETLD LDLIYKNVDK EFAKDGHAEG 101 ENIYTDYLVK ELLRYFKQDF FKWCNKPDCN HCGQNTSENM TPLGSQGPNG 151 EESKFNCGTV EIYKCNRCGN ITRFPRYNDP IKLLETRKGR CGEWCNLFTL 201 ILKSFGLDVR YVWNREDHVW CEYFSNFLNR WVHVDSCEQS FDQPYIYSIN 251 WNKKMSYCIA FGKDGVVDVS KRYILQNELP RDQIKEEDLK FLCQFITKRL 301 RYSLNDDEIY QLACRDEQEQ IELIRGKTQE TKSESVSAAS KSSNRGRESG 351 SADWKAQRGE DGK*

Amino Acid Sequence (or in FASTA format)

       1  MGEVYEKNNI DFDSIAKMLL IKYKDFILSK FKKAAPVENI RFQNLVHTNQ
      51  FAQGVLGQSQ HLCTVYDNPS WHSIVLETLD LDLIYKNVDK EFAKDGHAEG
     101  ENIYTDYLVK ELLRYFKQDF FKWCNKPDCN HCGQNTSENM TPLGSQGPNG
     151  EESKFNCGTV EIYKCNRCGN ITRFPRYNDP IKLLETRKGR CGEWCNLFTL
     201  ILKSFGLDVR YVWNREDHVW CEYFSNFLNR WVHVDSCEQS FDQPYIYSIN
     251  WNKKMSYCIA FGKDGVVDVS KRYILQNELP RDQIKEEDLK FLCQFITKRL
     301  RYSLNDDEIY QLACRDEQEQ IELIRGKTQE TKSESVSAAS KSSNRGRESG
     351  SADWKAQRGE DGK*

external links for Png1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Png1p

1)	Suzuki T, et al. (2000) PNG1, a yeast gene encoding a highly conserved peptide:N-glycanase. J Cell Biol 149(5):1039-52
2)	Biswas S, et al. (2004) The N-terminus of yeast peptide: N-glycanase interacts with the DNA repair protein Rad23. Biochem Biophys Res Commun 323(1):149-55
3)	Wang S, et al. (2009) N-terminal deletion of Peptide:N-glycanase results in enhanced deglycosylation activity. PLoS One 4(12):e8335
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41