MOT1/YPL082C Protein Information Help

Standard Name Mot1p 1
Systematic Name Ypl082cp
Alias Bur3p 2 , Lpf4p
ORF Classification Verified
Description Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA (1, 3, 4, 5, 6, 7) Also known as: BTAF1 8
Name Description Modifier of Transcription 1
Experimental Data
Molecules/cell 6560 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,867
Molecular Weight (Da) 209,975
Isoelectric Point (pI) 6.54

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mot1p (InterPro)
Physical Interactions There are 201 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052F62 | P32333
MIPS: YPL082C
NCBI: 1147612 | 171965 | 417308 | 6325175 | NP_015243.1
GenBank/EMBL/DDBJ: DAA11351.1 | M83224 | U41849
External Classifications EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides]
Amino Acid Sequence (or in FASTA format)
 
       1  MTSRVSRLDR QVILIETGST QVVRNMAADQ MGDLAKQHPE DILSLLSRVY
      51  PFLLVKKWET RVTAARAVGG IVAHAPSWDP NESDLVGGTN EGSPLDNAQV
     101  KLEHEMKIKL EEATQNNQLN LLQEDHHLSS LSDWKLNEIL KSGKVLLASS
     151  MNDYNVLGKA DDNIRKQAKT DDIKQETSML NASDKANENK SNANKKSARM
     201  LAMARRKKKM SAKNTPKHPV DITESSVSKT LLNGKNMTNS AASLATSPTS
     251  NQLNPKLEIT EQADESKLMI ESTVRPLLEQ HEIVAGLVWQ FQGIYELLLD
     301  NLMSENWEIR HGAALGLREL VKKHAYGVSR VKGNTREENN LRNSRSLEDL
     351  ASRLLTVFAL DRFGDYVYDT VVAPVRESVA QTLAALLIHL DSTLSIKIFN
     401  CLEQLVLQDP LQTGLPNKIW EATHGGLLGI RYFVSIKTNF LFAHGLLENV
     451  VRIVLYGLNQ SDDDVQSVAA SILTPITSEF VKLNNSTIEI LVTTIWSLLA
     501  RLDDDISSSV GSIMDLLAKL CDHQEVLDIL KNKALEHPSE WSFKSLVPKL
     551  YPFLRHSISS VRRAVLNLLI AFLSIKDDST KNWLNGKVFR LVFQNILLEQ
     601  NPELLQLSFD VYVALLEHYK VKHTEKTLDH VFSKHLQPIL HLLNTPVGEK
     651  GKNYAMESQY ILKPSQHYQL HPEKKRSISE TTTDSDIPIP KNNEHINIDA
     701  PMIAGDITLL GLDVILNTRI MGAKAFALTL SMFQDSTLQS FFTNVLVRCL
     751  ELPFSTPRML AGIIVSQFCS SWLQKHPEGE KLPSFVSEIF SPVMNKQLLN
     801  RDEFPVFREL VPSLKALRTQ CQSLLATFVD VGMLPQYKLP NVAIVVQGET
     851  EAGPHAFGVE TAEKVYGEYY DKMFKSMNNS YKLLAKKPLE DSKHRVLMAI
     901  NSAKESAKLR TGSILANYAS SILLFDGLPL KLNPIIRSLM DSVKEERNEK
     951  LQTMAGESVV HLIQQLLENN KVNVSGKIVK NLCGFLCVDT SEVPDFSVNA
    1001  EYKEKILTLI KESNSIAAQD DINLAKMSEE AQLKRKGGLI TLKILFEVLG
    1051  PSILQKLPQL RSILFDSLSD HENEEASKVD NEQGQKIVDS FGVLRALFPF
    1101  MSDSLRSSEV FTRFPVLLTF LRSNLSVFRY SAARTFADLA KISSVEVMAY
    1151  TIREILPLMN SAGSLSDRQG STELIYHLSL SMETDVLPYV IFLIVPLLGR
    1201  MSDSNEDVRN LATTTFASII KLVPLEAGIA DPKGLPEELV ASRERERDFI
    1251  QQMMDPSKAK PFKLPIAIKA TLRKYQQDGV NWLAFLNKYH LHGILCDDMG
    1301  LGKTLQTICI IASDQYLRKE DYEKTRSVES RALPSLIICP PSLTGHWENE
    1351  FDQYAPFLKV VVYAGGPTVR LTLRPQLSDA DIIVTSYDVA RNDLAVLNKT
    1401  EYNYCVLDEG HIIKNSQSKL AKAVKEITAN HRLILTGTPI QNNVLELWSL
    1451  FDFLMPGFLG TEKMFQERFA KPIAASRNSK TSSKEQEAGV LALEALHKQV
    1501  LPFMLRRLKE DVLSDLPPKI IQDYYCELGD LQKQLYMDFT KKQKNVVEKD
    1551  IENSEIADGK QHIFQALQYM RKLCNHPALV LSPNHPQLAQ VQDYLKQTGL
    1601  DLHDIINAPK LSALRTLLFE CGIGEEDIDK KASQDQNFPI QNVISQHRAL
    1651  IFCQLKDMLD MVENDLFKKY MPSVTYMRLD GSIDPRDRQK VVRKFNEDPS
    1701  IDCLLLTTKV GGLGLNLTGA DTVIFVEHDW NPMNDLQAMD RAHRIGQKKV
    1751  VNVYRIITKG TLEEKIMGLQ KFKMNIASTV VNQQNSGLAS MDTHQLLDLF
    1801  DPDNVTSQDN EEKNNGDSQA AKGMEDIANE TGLTGKAKEA LGELKELWDP
    1851  SQYEEEYNLD TFIKTLR*

external links for Mot1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Mot1p
1) Davis JL, et al.  (1992) A presumptive helicase (MOT1 gene product) affects gene expression and is required for viability in the yeast Saccharomyces cerevisiae. Mol Cell Biol 12(4):1879-92
2) Prelich G  (1997) Saccharomyces cerevisiae BUR6 encodes a DRAP1/NC2alpha homolog that has both positive and negative roles in transcription in vivo. Mol Cell Biol 17(4):2057-65
3) Collart MA  (1996) The NOT, SPT3, and MOT1 genes functionally interact to regulate transcription at core promoters. Mol Cell Biol 16(12):6668-76
4) Auble DT, et al.  (1997) Molecular analysis of the SNF2/SWI2 protein family member MOT1, an ATP-driven enzyme that dissociates TATA-binding protein from DNA. Mol Cell Biol 17(8):4842-51
5) Muldrow TA, et al.  (1999) MOT1 can activate basal transcription in vitro by regulating the distribution of TATA binding protein between promoter and nonpromoter sites. Mol Cell Biol 19(4):2835-45
6) Gumbs OH, et al.  (2003) High-affinity DNA binding by a Mot1p-TBP complex: implications for TAF-independent transcription. EMBO J 22(12):3131-41
7) Moyle-Heyrman G, et al.  (2012) Two-step mechanism for modifier of transcription 1 (Mot1) enzyme-catalyzed displacement of TATA-binding protein (TBP) from DNA. J Biol Chem 287(12):9002-12
8) Tora L  (2002) A unified nomenclature for TATA box binding protein (TBP)-associated factors (TAFs) involved in RNA polymerase II transcription. Genes Dev 16(6):673-5
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41