GPI2/YPL076W Protein Information

Standard Name	Gpi2p 1
Systematic Name	Ypl076wp
Alias	Gcr4p 2
ORF Classification	Verified
Description	Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein (1, 3)
Name Description	GlycosylPhosphatidylInositol anchor biosynthesis 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	280
Molecular Weight (Da)	32,577
Isoelectric Point (pI)	10.24

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gpi2p (InterPro)
Transmembrane Domains	There are 6 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 65 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012B9CF \| P46961 MIPS: YPL076W NCBI: 1072321 \| 1147617 \| 1346162 \| 51013373 \| 6325181 \| 841378 \| NP_015249.1 GenBank/EMBL/DDBJ: DAA11356.1 \| AY692961 \| D29645 \| U23788 \| U41849
External Classifications	EC: 2.4.1.198 [Phosphatidylinositol N-acetylglucosaminyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MTRSPWKRLL WLKQEYPDNY TDPSFIELRA RQKAESNQKS DRKLSEAARA 51 QIRLDFISFY QTILNTSFIY ITFTYIYYYG FDPIPPTIFL SFITLIISRT 101 KVDPLLSSFM DVKSSLIITF AMLTLSPVLK SLSKTTASDS IWTLSFWLTL 151 WYIFVISSTK SKDKPSNLST NILVALVAVL SSRLSTTIDV FCFLLICIQL 201 NIILPTYLSV TNKVVPIISN IIVYSFLNVA LGWIYMLLIF FASVFYITVL 251 PKWFIYWKIN YHKRDNDLLS TWDARTPILD *

Amino Acid Sequence (or in FASTA format)

       1  MTRSPWKRLL WLKQEYPDNY TDPSFIELRA RQKAESNQKS DRKLSEAARA
      51  QIRLDFISFY QTILNTSFIY ITFTYIYYYG FDPIPPTIFL SFITLIISRT
     101  KVDPLLSSFM DVKSSLIITF AMLTLSPVLK SLSKTTASDS IWTLSFWLTL
     151  WYIFVISSTK SKDKPSNLST NILVALVAVL SSRLSTTIDV FCFLLICIQL
     201  NIILPTYLSV TNKVVPIISN IIVYSFLNVA LGWIYMLLIF FASVFYITVL
     251  PKWFIYWKIN YHKRDNDLLS TWDARTPILD *

external links for Gpi2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Gpi2p

1)	Leidich SD, et al. (1995) Temperature-sensitive yeast GPI anchoring mutants gpi2 and gpi3 are defective in the synthesis of N-acetylglucosaminyl phosphatidylinositol. Cloning of the GPI2 gene. J Biol Chem 270(22):13029-35
2)	Uemura H and Jigami Y (1995) A new essential gene GCR4 located in the upstream region of GCR1. Yeast 11(11):1093-101
3)	Inoue N, et al. (1996) PIG-C, one of the three human genes involved in the first step of glycosylphosphatidylinositol biosynthesis is a homologue of Saccharomyces cerevisiae GPI2. Biochem Biophys Res Commun 226(1):193-9