TIM50/YPL063W Protein Information

Standard Name	Tim50p 1
Systematic Name	Ypl063wp
ORF Classification	Verified
Description	Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel (2, 3, 4, 5)
Name Description	Translocase of the Inner Mitochondrial membrane

Experimental Data
Molecules/cell	2400 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	476
Molecular Weight (Da)	55,098
Isoelectric Point (pI)	7.29

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tim50p (InterPro)
Physical Interactions	There are 74 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000069819 \| Q02776 MIPS: YPL063W NCBI: 1079679 \| 6325194 \| 74676305 \| NP_015262.1 \| NM_001183877.1 GenBank/EMBL/DDBJ: DAA11368.1 \| U39205

Amino Acid Sequence (or in FASTA format)
1 MLSILRNSVR LNSRALRVVP SAANTLTSVQ ASRRLLTSYS SFLQKETKDD 51 KPKSILTDDM LFKAGVDVDE KGQGKNEETS GEGGEDKNEP SSKSEKSRRK 101 RQTSTDIKRE KYANWFYIFS LSALTGTAIY MARDWEPQES EELKKDIDNG 151 YTLSLMYKRF KARFNSMFTY FQEPPFPDLL PPPPPPPYQR PLTLVITLED 201 FLVHSEWSQK HGWRTAKRPG ADYFLGYLSQ YYEIVLFSSN YMMYSDKIAE 251 KLDPIHAFVS YNLFKEHCVY KDGVHIKDLS KLNRDLSKVI IIDTDPNSYK 301 LQPENAIPME PWNGEADDKL VRLIPFLEYL ATQQTKDVRP ILNSFEDKKN 351 LAEEFDHRVK KLKDKFYGDH KSGGNWAMTA LGLGNSLGGS TKFPLDLIHE 401 EGQKNYLMFM KMIEEEKEKI RIQQEQMGGQ TFTLKDYVEG NLPSPEEQMK 451 IQLEKQKEVD ALFEEEKKKK KIAESK*

Amino Acid Sequence (or in FASTA format)

       1  MLSILRNSVR LNSRALRVVP SAANTLTSVQ ASRRLLTSYS SFLQKETKDD
      51  KPKSILTDDM LFKAGVDVDE KGQGKNEETS GEGGEDKNEP SSKSEKSRRK
     101  RQTSTDIKRE KYANWFYIFS LSALTGTAIY MARDWEPQES EELKKDIDNG
     151  YTLSLMYKRF KARFNSMFTY FQEPPFPDLL PPPPPPPYQR PLTLVITLED
     201  FLVHSEWSQK HGWRTAKRPG ADYFLGYLSQ YYEIVLFSSN YMMYSDKIAE
     251  KLDPIHAFVS YNLFKEHCVY KDGVHIKDLS KLNRDLSKVI IIDTDPNSYK
     301  LQPENAIPME PWNGEADDKL VRLIPFLEYL ATQQTKDVRP ILNSFEDKKN
     351  LAEEFDHRVK KLKDKFYGDH KSGGNWAMTA LGLGNSLGGS TKFPLDLIHE
     401  EGQKNYLMFM KMIEEEKEKI RIQQEQMGGQ TFTLKDYVEG NLPSPEEQMK
     451  IQLEKQKEVD ALFEEEKKKK KIAESK*

external links for Tim50p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Tim50p

1)	Geissler A, et al. (2002) The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel. Cell 111(4):507-18
2)	Chacinska A, et al. (2005) Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell 120(6):817-29
3)	Mokranjac D, et al. (2009) Role of Tim50 in the transfer of precursor proteins from the outer to the inner membrane of mitochondria. Mol Biol Cell 20(5):1400-7
4)	Gevorkyan-Airapetov L, et al. (2009) Interaction of Tim23 with Tim50 Is Essential for Protein Translocation by the Mitochondrial TIM23 Complex. J Biol Chem 284(8):4865-72
5)	Meinecke M, et al. (2006) Tim50 maintains the permeability barrier of the mitochondrial inner membrane. Science 312(5779):1523-6
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41