PDR12/YPL058C Protein Information Help

Standard Name Pdr12p
Systematic Name Ypl058cp
ORF Classification Verified
Description Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity (1, 2, 3, 4)
Name Description Pleiotropic Drug Resistance
Experimental Data
Molecules/cell 752 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,511
Molecular Weight (Da) 171,064
Isoelectric Point (pI) 6.82

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pdr12p (InterPro)
Transmembrane Domains There are 11 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 58 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00000327B8 | Q02785
MIPS: YPL058C
NCBI: 1079684 | 6093664 | 6325199 | NP_015267.1
GenBank/EMBL/DDBJ: DAA11372.1 | U39205
External Classifications TC: 3.A.1.205 [The Pleiotropic Drug Resistance (PDR) Family]
Amino Acid Sequence (or in FASTA format)
 
       1  MSSTDEHIEK DISSRSNHDD DYANSVQSYA ASEGQVDNED LAATSQLSRH
      51  LSNILSNEEG IERLESMARV ISHKTKKEMD SFEINDLDFD LRSLLHYLRS
     101  RQLEQGIEPG DSGIAFKNLT AVGVDASAAY GPSVEEMFRN IASIPAHLIS
     151  KFTKKSDVPL RNIIQNCTGV VESGEMLFVV GRPGAGCSTF LKCLSGETSE
     201  LVDVQGEFSY DGLDQSEMMS KYKGYVIYCP ELDFHFPKIT VKETIDFALK
     251  CKTPRVRIDK MTRKQYVDNI RDMWCTVFGL RHTYATKVGN DFVRGVSGGE
     301  RKRVSLVEAQ AMNASIYSWD NATRGLDAST ALEFAQAIRT ATNMVNNSAI
     351  VAIYQAGENI YELFDKTTVL YNGRQIYFGP ADKAVGYFQR MGWVKPNRMT
     401  SAEFLTSVTV DFENRTLDIK PGYEDKVPKS SSEFEEYWLN SEDYQELLRT
     451  YDDYQSRHPV NETRDRLDVA KKQRLQQGQR ENSQYVVNYW TQVYYCMIRG
     501  FQRVKGDSTY TKVYLSSFLI KALIIGSMFH KIDDKSQSTT AGAYSRGGML
     551  FYVLLFASVT SLAEIGNSFS SRPVIVKHKS YSMYHLSAES LQEIITEFPT
     601  KFVAIVILCL ITYWIPFMKY EAGAFFQYIL YLLTVQQCTS FIFKFVATMS
     651  KSGVDAHAVG GLWVLMLCVY AGFVLPIGEM HHWIRWLHFI NPLTYAFESL
     701  VSTEFHHREM LCSALVPSGP GYEGISIANQ VCDAAGAVKG NLYVSGDSYI
     751  LHQYHFAYKH AWRNWGVNIV WTFGYIVFNV ILSEYLKPVE GGGDLLLYKR
     801  GHMPELGTEN ADARTASREE MMEALNGPNV DLEKVIAEKD VFTWNHLDYT
     851  IPYDGATRKL LSDVFGYVKP GKMTALMGES GAGKTTLLNV LAQRINMGVI
     901  TGDMLVNAKP LPASFNRSCG YVAQADNHMA ELSVRESLRF AAELRQQSSV
     951  PLEEKYEYVE KIITLLGMQN YAEALVGKTG RGLNVEQRKK LSIGVELVAK
    1001  PSLLLFLDEP TSGLDSQSAW SIVQFMRALA DSGQSILCTI HQPSATLFEQ
    1051  FDRLLLLKKG GKMVYFGDIG PNSETLLKYF ERQSGMKCGV SENPAEYILN
    1101  CIGAGATASV NSDWHDLWLA SPECAAARAE VEELHRTLPG RAVNDDPELA
    1151  TRFAASYMTQ IKCVLRRTAL QFWRSPVYIR AKFFECVACA LFVGLSYVGV
    1201  NHSVGGAIEA FSSIFMLLLI ALAMINQLHV FAYDSRELYE VREAASNTFH
    1251  WSVLLLCHAA VENFWSTLCQ FMCFICYYWP AQFSGRASHA GFFFFFYVLI
    1301  FPLYFVTYGL WILYMSPDVP SASMINSNLF AAMLLFCGIL QPREKMPAFW
    1351  RRLMYNVSPF TYVVQALVTP LVHNKKVVCN PHEYNIMDPP SGKTCGEFLS
    1401  TYMDNNTGYL VNPTATENCQ YCPYTVQDQV VAKYNVKWDH RWRNFGFMWA
    1451  YICFNIAAML ICYYVVRVKV WSLKSVLNFK KWFNGPRKER HEKDTNIFQT
    1501  VPGDENKITK K*

external links for Pdr12p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome TCDB

GeneMANIA YeastRC Structure Prediction (Seattle)

References cited on this page View Complete Literature Guide for Pdr12p
1) Holyoak CD, et al.  (2000) Loss of Cmk1 Ca(2+)-calmodulin-dependent protein kinase in yeast results in constitutive weak organic acid resistance, associated with a post-transcriptional activation of the Pdr12 ATP-binding cassette transporter. Mol Microbiol 37(3):595-605
2) Holyoak CD, et al.  (1999) The Saccharomyces cerevisiae weak-acid-inducible ABC transporter Pdr12 transports fluorescein and preservative anions from the cytosol by an energy-dependent mechanism. J Bacteriol 181(15):4644-52
3) Decottignies A and Goffeau A  (1997) Complete inventory of the yeast ABC proteins. Nat Genet 15(2):137-45
4) Schuller C, et al.  (2004) Global phenotypic analysis and transcriptional profiling defines the weak acid stress response regulon in Saccharomyces cerevisiae. Mol Biol Cell 15(2):706-20
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41