DIG1/YPL049C Protein Information Help

Standard Name Dig1p 1
Systematic Name Ypl049cp
Alias Rst1p 2
ORF Classification Verified
Description MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG1 has a paralog, DIG2, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7)
Name Description Down-regulator of Invasive Growth 1
Experimental Data
Molecules/cell 1460 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 452
Molecular Weight (Da) 49,356
Isoelectric Point (pI) 9.31

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Dig1p (InterPro)
Physical Interactions There are 91 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000053294 | Q03063
MIPS: YPL049C
NCBI: 1171410 | 41016991 | 6325208 | NP_015276.1
GenBank/EMBL/DDBJ: DAA11381.1 | U44030
Amino Acid Sequence (or in FASTA format)
 
       1  MAVSARLRTT AEDTSIAKST QDPIGDTEIS VANAKGSSDS NIKNSPGGNS
      51  VGQESELEHV PEEDDSGDKE ADHEDSETAT AKKRKAQPLK NPKKSLKRGR
     101  VPAPLNLSDS NTNTHGGNIK DGNLASSNSA HFPPVANQNV KSAPAQVTQH
     151  SKFQPRVQYL GKASSRQSIQ VNNSSNSYGK PHMPSAGIMS AMNPYMPMNR
     201  YIMSPYYNPY GIPPPHMLNK PIMTPYVSYP YPMGPRTSIP YAMQGGNARP
     251  YEENEYSASN YRNKRVNDSY DSPLSGTAST GKTRRSEEGS RNSSVGSSAN
     301  AGPTQQRADL RPADMIPAEE YHFERDALLS ANTKARSAST STSTSTSTNR
     351  DRSSWHEAEP NKDEEEGTDL AIEDGAVPTP TFTTFQRTSQ PQQQSPSLLQ
     401  GEIRLSSHIF AFEFPLSSSN VDKKMFMSIC NKVWNESKEL TKKSSSHHRT
     451  GK*

external links for Dig1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome


DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Dig1p
1) Cook JG, et al.  (1996) Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. Genes Dev 10(22):2831-48
2) Tedford K, et al.  (1997) Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates. Curr Biol 7(4):228-38
3) Olson KA, et al.  (2000) Two regulators of Ste12p inhibit pheromone-responsive transcription by separate mechanisms. Mol Cell Biol 20(12):4199-209
4) Bardwell L, et al.  (1998) Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins. Proc Natl Acad Sci U S A 95(26):15400-5
5) Gelli A  (2002) Rst1 and Rst2 are required for the a/alpha diploid cell type in yeast. Mol Microbiol 46(3):845-54
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) McCullagh E, et al.  (2010) Coordinate control of gene expression noise and interchromosomal interactions in a MAP kinase pathway. Nat Cell Biol 12(10):954-962
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41