VPS16/YPL045W Protein Information Help

Standard Name Vps16p 1, 2
Systematic Name Ypl045wp
Alias Svl6p 3 , Vam9p , Vpt16p 1
ORF Classification Verified
Description Subunit of the vacuole fusion and protein sorting HOPS complex and the CORVET tethering complex; part of the Class C Vps complex essential for membrane docking and fusion at Golgi-to-endosome and endosome-to-vacuole protein transport stages (4, 5, 6)
Name Description Vacuolar Protein Sorting 1, 2
Experimental Data
Molecules/cell 1300 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 798
Molecular Weight (Da) 92,740
Isoelectric Point (pI) 7.04

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Vps16p (InterPro)
Physical Interactions There are 87 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052FFD | Q03308
MIPS: YPL045W
NCBI: 1171414 | 173181 | 2507153 | 6325212 | NP_015280.1
GenBank/EMBL/DDBJ: DAA11385.1 | L07327 | U44030
Amino Acid Sequence (or in FASTA format)
 
       1  MKNPSFDWER LKDVFYRSRA IGELKWPTQY EEFKCALSLT VIAVEIQDFI
      51  QVYNYFGQLL GKINLQRIHE DIIKFEFDKD EKLILVTKSS IKIVKGWSPL
     101  TIESVPLQDP TIDTIWDYHN GIMLLAKSRD IYKLNGNEWE LLYENKDKKY
     151  NLLTKNHWSC NDDSIILLDV DHVYQVSTSN GALLKLITDS SWHKVTISSR
     201  GFICLYNMKD NKLQIFRDPA RILMEHNLDS TPDDICWCGN DTVACSFEDE
     251  IKLYGPDGLY VTFWYPFTVT NLRAEVDGLK VITTEKIYFL SRVQPQTSNI
     301  FRIGSTEPGA MLVDSFSLLE DHAPKAIEIL KNFVLEKGVL DCIAAAIDEF
     351  EPKLQKMLLN AASYGKASLQ YKSFDASIFV NACNTIKLLN CFRSFGIFLT
     401  VEEYRCISLK GVIDRLLKYH RYYECIQICK LANERFLLGY VFTEWAKDKI
     451  KGSPDMEDDE LLDKIKSRLS VIDMTDTLQM VAVAKVAYLE GRFQLSRNLA
     501  LLEKNEEARI EQLYNLDDDS IALKECIKVQ NYSLTISLLI ALSKKLTNSQ
     551  LTKLLIIDMF NNPLYLYYMR MDKAYLYDFY RQTDRFIDLA HVLLQQGKEQ
     601  QSLHSFLPQI KDLYSQVQNS EVVNNTIEQL QRQEKLWIYQ ESLGKRFAIS
     651  FTNMTLDQTL SKLIETGQDK QVKEIVKKFK ISEKKLYHLK CKTLVEAKKF
     701  DELLQFAQSR KSPIGYMPFY TYLKSRGHMD KASPYVNMIP GLSYQEKKKL
     751  YVECRGFRDA IQLAGKEKDI PGLKEIYNII PPNEPELKAL ANETMSRI*

external links for Vps16p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Vps16p
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Zheng B, et al.  (1998) Isolation of yeast mutants defective for localization of vacuolar vital dyes. Proc Natl Acad Sci U S A 95(20):11721-6
4) Seals DF, et al.  (2000) A Ypt/Rab effector complex containing the Sec1 homolog Vps33p is required for homotypic vacuole fusion. Proc Natl Acad Sci U S A 97(17):9402-7
5) Rieder SE and Emr SD  (1997) A novel RING finger protein complex essential for a late step in protein transport to the yeast vacuole. Mol Biol Cell 8(11):2307-27
6) Peterson MR and Emr SD  (2001) The class C Vps complex functions at multiple stages of the vacuolar transport pathway. Traffic 2(7):476-86
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41