CHL1/YPL008W Protein Information

Standard Name	Chl1p
Systematic Name	Ypl008wp
Alias	Ctf1p 1 , Lpa9p , Mcm12p 2
ORF Classification	Verified
Description	Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome (3, 4, 5, 6, 7)
Name Description	CHromosome Loss 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	861
Molecular Weight (Da)	98,805
Isoelectric Point (pI)	7.91

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Chl1p (InterPro)
Physical Interactions	There are 18 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000127838 \| P22516 MIPS: YPL008W NCBI: 116351 \| 1314068 \| 3538 \| 6325249 \| 683782 \| 965085 \| NP_015317.1 GenBank/EMBL/DDBJ: DAA11420.1 \| U33335 \| X56584 \| Z48483 \| Z71255
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.13 [RNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MDKKEYSETF YHPYKPYDIQ VQLMETVYRV LSEGKKIAIL ESPTGTGKTL 51 SLICATMTWL RMNKADIFTR METNIKTNED DSENLSDDEP DWVIDTYRKS 101 VLQEKVDLLN DYEKHLNEIN TTSCKQLKTM CDLDKEHGRY KSVDPLRKKR 151 KGARHLDVSL EEQDFIPRPY ESDSENNDTS KSTRGGRISD KDYKLSELNS 201 QIITLLDKID GKVSRDPNNG DRFDVTNQNP VKIYYASRTY SQLGQFTSQL 251 RLPSFPSSFR DKVPDEKVKY LPLASKKQLC INPKVMKWKT LEAINDACAD 301 LRHSKEGCIF YQNTNEWRHC PDTLALRDMI FSEIQDIEDL VPLGKSLGIC 351 PYYASREALP IAEVVTLPYQ YLLSESTRSS LQINLENSIV IIDEAHNLIE 401 TINSIYSSQI SLEDLKNCHK GIVTYFNKFK SRLNPGNRVN LLKLNSLLMT 451 LIQFIVKNFK KIGQEIDPND MFTGSNIDTL NIHKLLRYIK VSKIAYKIDT 501 YNQALKEEES SKNENPIKET HKKSVSSQPL LFKVSQFLYC LTNLTSEGQF 551 FFEKNYSIKY MLLEPSKPFE SILNQAKCVV LAGGTMEPMS EFLSNLLPEV 601 PSEDITTLSC NHVIPKENLQ TYITNQPELE FTFEKRMSPS LVNNHLFQFF 651 VDLSKAVPKK GGIVAFFPSY QYLAHVIQCW KQNDRFATLN NVRKIFYEAK 701 DGDDILSGYS DSVAEGRGSL LLAIVGGKLS EGINFQDDLC RAVVMVGLPF 751 PNIFSGELIV KRKHLAAKIM KSGGTEEEAS RATKEFMENI CMKAVNQSVG 801 RAIRHANDYA NIYLLDVRYN RPNFRKKLSR WVQDSINSEH TTHQVISSTR 851 KFFSMRSLNS R*

Amino Acid Sequence (or in FASTA format)

       1  MDKKEYSETF YHPYKPYDIQ VQLMETVYRV LSEGKKIAIL ESPTGTGKTL
      51  SLICATMTWL RMNKADIFTR METNIKTNED DSENLSDDEP DWVIDTYRKS
     101  VLQEKVDLLN DYEKHLNEIN TTSCKQLKTM CDLDKEHGRY KSVDPLRKKR
     151  KGARHLDVSL EEQDFIPRPY ESDSENNDTS KSTRGGRISD KDYKLSELNS
     201  QIITLLDKID GKVSRDPNNG DRFDVTNQNP VKIYYASRTY SQLGQFTSQL
     251  RLPSFPSSFR DKVPDEKVKY LPLASKKQLC INPKVMKWKT LEAINDACAD
     301  LRHSKEGCIF YQNTNEWRHC PDTLALRDMI FSEIQDIEDL VPLGKSLGIC
     351  PYYASREALP IAEVVTLPYQ YLLSESTRSS LQINLENSIV IIDEAHNLIE
     401  TINSIYSSQI SLEDLKNCHK GIVTYFNKFK SRLNPGNRVN LLKLNSLLMT
     451  LIQFIVKNFK KIGQEIDPND MFTGSNIDTL NIHKLLRYIK VSKIAYKIDT
     501  YNQALKEEES SKNENPIKET HKKSVSSQPL LFKVSQFLYC LTNLTSEGQF
     551  FFEKNYSIKY MLLEPSKPFE SILNQAKCVV LAGGTMEPMS EFLSNLLPEV
     601  PSEDITTLSC NHVIPKENLQ TYITNQPELE FTFEKRMSPS LVNNHLFQFF
     651  VDLSKAVPKK GGIVAFFPSY QYLAHVIQCW KQNDRFATLN NVRKIFYEAK
     701  DGDDILSGYS DSVAEGRGSL LLAIVGGKLS EGINFQDDLC RAVVMVGLPF
     751  PNIFSGELIV KRKHLAAKIM KSGGTEEEAS RATKEFMENI CMKAVNQSVG
     801  RAIRHANDYA NIYLLDVRYN RPNFRKKLSR WVQDSINSEH TTHQVISSTR
     851  KFFSMRSLNS R*

external links for Chl1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Chl1p

1)	Spencer F, et al. (1990) Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics 124(2):237-49
2)	Laha S, et al. (2006) The budding yeast protein Chl1p is required to preserve genome integrity upon DNA damage in S-phase. Nucleic Acids Res 34(20):5880-91
3)	Skibbens RV (2004) Chl1p, a DNA helicase-like protein in budding yeast, functions in sister-chromatid cohesion. Genetics 166(1):33-42
4)	Das SP and Sinha P (2005) The budding yeast protein Chl1p has a role in transcriptional silencing, rDNA recombination, and aging. Biochem Biophys Res Commun 337(1):167-72
5)	Wu Y, et al. (2009) Welcome the family of FANCJ-like helicases to the block of genome stability maintenance proteins. Cell Mol Life Sci 66(7):1209-22
6)	Wu Y, et al. (2012) Biochemical characterization of Warsaw breakage syndrome helicase. J Biol Chem 287(2):1007-21
7)	Ward TA, et al. (2012) Components of a fanconi-like pathway control pso2-independent DNA interstrand crosslink repair in yeast. PLoS Genet 8(8):e1002884
8)	Liras P, et al. (1978) Characterization of a mutation in yeast causing nonrandom chromosome loss during mitosis. Genetics 88(4 Pt 1):651-71