FRE5/YOR384W Protein Information Help

Standard Name Fre5p
Systematic Name Yor384wp
ORF Classification Verified
Description Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies (1, 2, 3, 4)
Name Description Ferric REductase
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 694
Molecular Weight (Da) 80,292
Isoelectric Point (pI) 8.89

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Fre5p (InterPro)
Transmembrane Domains There are 6 total predicted transmembrane domains (TMHMM)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 1 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012ABF8 | Q08908
MIPS: YOR384W
NCBI: 1420827 | 3913675 | 51013367 | 6324960 | NP_015029.1
GenBank/EMBL/DDBJ: DAA11143.1 | AY692958 | Z75292
External Classifications EC: 1.16.1.-
EC: 1.16.1.7 [Ferric-chelate reductase (NADH)] TC: 1.A.20.5
Amino Acid Sequence (or in FASTA format)
 
       1  MLFARLVLLL VYLAPGSLAK PASTKKRTQW DQIAIDACAK ELESHKFDTD
      51  VKGRHATLCT YEPALGSWLH CAKDVLDSRK KSKKIFEKTF SKINQYCHDY
     101  HKDEVVSNEE YYRIFANASL FIRPLDEVKE NIRYPVTPNK ASLDRWVWAY
     151  FGPLDNIDKG NVYGVTICLY WIGVLFIAAV YHFLNFSRLK QTVFKNKVSA
     201  FLRGHYVLPA LVHNHAMSVG RWFFIGLVPT RLETLVLFGY VLLHGFLLSS
     251  YNFDHNELLS DRRSQVLIFL SDRAGILAFA HFPLIVLFGG KNSTMTWLTG
     301  IRYTAFITYH KWLGRFMLVD CTIHAIGYTY HAYIENYWKY VKYSDLWTSG
     351  RHAMIIVGIL VFFSFFFFRR HYYELFVITH IILAIGFFHA CWKHCYKLGW
     401  GEWIMACALF WIADRILRLI KIAIFGMPWA KLKLCGESMI EVRISKSSKW
     451  WKAEPGQYIY LYFLRPKIFW QSHPFTVMDS LVEDGELVVV ITVKNGLTKK
     501  LQEYLLESEG YTEMRVLAEG PYGQSTRTHL FESLLFIAGG AGVPGPLSMA
     551  IKAGRQVKSN DSHQMIKFVW SVRNLDLLEV YRKEIMVLKE LNIDTKIYFT
     601  GERKDESNTE EGAIANMSTE GRLLTTSKSA EMITDFGRPN IDEIIEEAVS
     651  GAKSLLVTCC GSEGFVDKTR ELTAKRVLEH GDKWIEYVEE FQNW*

external links for Fre5p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS
YOGY CYC2008 (complexes) Pfam domains

Complexome TCDB

DIP YeastRC Structure Prediction (Seattle)

GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Fre5p
1) Martins LJ, et al.  (1998) Metalloregulation of FRE1 and FRE2 homologs in Saccharomyces cerevisiae. J Biol Chem 273(37):23716-21
2) Georgatsou E and Alexandraki D  (1999) Regulated expression of the Saccharomyces cerevisiae Fre1p/Fre2p Fe/Cu reductase related genes. Yeast 15(7):573-84
3) Yun CW, et al.  (2001) The role of the FRE family of plasma membrane reductases in the uptake of siderophore-iron in Saccharomyces cerevisiae. J Biol Chem 276(13):10218-23
4) Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54