GDH1/YOR375C Protein Information

Standard Name	Gdh1p
Systematic Name	Yor375cp
Alias	Ure1p , Dhe4p
ORF Classification	Verified
Description	NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication (1, 2, 3) Also known as: GDH-A , GDHA
Name Description	Glutamate DeHydrogenase

Experimental Data
Molecules/cell	77500 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	454
Molecular Weight (Da)	49,570
Isoelectric Point (pI)	5.5

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gdh1p (InterPro)
Physical Interactions	There are 28 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012930A \| P07262 MIPS: YOR375C NCBI: 1420810 \| 171592 \| 171594 \| 2506355 \| 398366421 \| NP_015020.3 GenBank/EMBL/DDBJ: DAA11134.1 \| M10590 \| M11297 \| Z75283
External Classifications	EC: 1.4.1.4 [Glutamate dehydrogenase (NADP(+))]

Amino Acid Sequence (or in FASTA format)
1 MSEPEFQQAY EEVVSSLEDS TLFEQHPEYR KVLPIVSVPE RIIQFRVTWE 51 NDKGEQEVAQ GYRVQYNSAK GPYKGGLRFH PSVNLSILKF LGFEQIFKNS 101 LTGLDMGGGK GGLCVDLKGR SNNEIRRICY AFMRELSRHI GQDTDVPAGD 151 IGVGGREIGY LFGAYRSYKN SWEGVLTGKG LNWGGSLIRP EATGYGLVYY 201 TQAMIDYATN GKESFEGKRV TISGSGNVAQ YAALKVIELG GTVVSLSDSK 251 GCIISETGIT SEQVADISSA KVNFKSLEQI VNEYSTFSEN KVQYIAGARP 301 WTHVQKVDIA LPCATQNEVS GEEAKALVAQ GVKFIAEGSN MGSTPEAIAV 351 FETARSTATG PSEAVWYGPP KAANLGGVAV SGLEMAQNSQ RITWTSERVD 401 QELKRIMINC FNECIDYAKK YTKDGKVLPS LVKGANIASF IKVSDAMFDQ 451 GDVF*

Amino Acid Sequence (or in FASTA format)

       1  MSEPEFQQAY EEVVSSLEDS TLFEQHPEYR KVLPIVSVPE RIIQFRVTWE
      51  NDKGEQEVAQ GYRVQYNSAK GPYKGGLRFH PSVNLSILKF LGFEQIFKNS
     101  LTGLDMGGGK GGLCVDLKGR SNNEIRRICY AFMRELSRHI GQDTDVPAGD
     151  IGVGGREIGY LFGAYRSYKN SWEGVLTGKG LNWGGSLIRP EATGYGLVYY
     201  TQAMIDYATN GKESFEGKRV TISGSGNVAQ YAALKVIELG GTVVSLSDSK
     251  GCIISETGIT SEQVADISSA KVNFKSLEQI VNEYSTFSEN KVQYIAGARP
     301  WTHVQKVDIA LPCATQNEVS GEEAKALVAQ GVKFIAEGSN MGSTPEAIAV
     351  FETARSTATG PSEAVWYGPP KAANLGGVAV SGLEMAQNSQ RITWTSERVD
     401  QELKRIMINC FNECIDYAKK YTKDGKVLPS LVKGANIASF IKVSDAMFDQ
     451  GDVF*

external links for Gdh1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
YGOB	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Gdh1p

1)	DeLuna A, et al. (2001) NADP-glutamate dehydrogenase isoenzymes of Saccharomyces cerevisiae. Purification, kinetic properties, and physiological roles. J Biol Chem 276(47):43775-83
2)	Riego L, et al. (2002) GDH1 expression is regulated by GLN3, GCN4, and HAP4 under respiratory growth. Biochem Biophys Res Commun 293(1):79-85
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41