RAD17/YOR368W Protein Information

Standard Name	Rad17p
Systematic Name	Yor368wp
ORF Classification	Verified
Description	Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins (1, 2, 3, 4)
Name Description	RADiation sensitive

Experimental Data
Molecules/cell	189 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	401
Molecular Weight (Da)	45,579
Isoelectric Point (pI)	5.02

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rad17p (InterPro)
Physical Interactions	There are 31 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000133012 \| P48581 MIPS: YOR368W NCBI: 1051278 \| 1346947 \| 1420796 \| 6324944 \| 950165 \| NP_015013.1 \| NM_001183788.1 GenBank/EMBL/DDBJ: DAA11127.1 \| U30796 \| U37460 \| Z75276
External Classifications	EC: 3.1.11.2 [Exodeoxyribonuclease III]

Amino Acid Sequence (or in FASTA format)
1 MRINSELANK FSASTVHLEH ITTALSCLTP FGSKDDVLIF IDADGLSFVR 51 ENNHVIKIQL LLSRELFMSY SYRNETEDHM KLCVKINHIL DSVSVMNRNS 101 DDIVECTLSY DGHGSPFVLI FEDSFISERV EYSTYLIKDF DTNGLELDRE 151 RISFEAIIKG EALHSALKDL KEIGCKECYV YAKTEANDEN VFALISKSQL 201 GFSKIKLPSN RSILEKLQVF DGDSTTVIDG FAVIGFFDFT SFDKIRKSTK 251 IASKVLFRMD VHGVLSVNIL SQTDDVIITD TTRPSNNRPG SIRQLQLPKD 301 YPGIVIEVCM LEKESIDEAA QTEIELLMET NELGNRNSFK KSTIRKRYGT 351 DKGNETSNDN LLQLNGKKIK LPSEEENNKN RESEDEENHC KYPTKDIPIF 401 F*

Amino Acid Sequence (or in FASTA format)

       1  MRINSELANK FSASTVHLEH ITTALSCLTP FGSKDDVLIF IDADGLSFVR
      51  ENNHVIKIQL LLSRELFMSY SYRNETEDHM KLCVKINHIL DSVSVMNRNS
     101  DDIVECTLSY DGHGSPFVLI FEDSFISERV EYSTYLIKDF DTNGLELDRE
     151  RISFEAIIKG EALHSALKDL KEIGCKECYV YAKTEANDEN VFALISKSQL
     201  GFSKIKLPSN RSILEKLQVF DGDSTTVIDG FAVIGFFDFT SFDKIRKSTK
     251  IASKVLFRMD VHGVLSVNIL SQTDDVIITD TTRPSNNRPG SIRQLQLPKD
     301  YPGIVIEVCM LEKESIDEAA QTEIELLMET NELGNRNSFK KSTIRKRYGT
     351  DKGNETSNDN LLQLNGKKIK LPSEEENNKN RESEDEENHC KYPTKDIPIF
     401  F*

external links for Rad17p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rad17p

1)	Parker AE, et al. (1998) A human homologue of the Schizosaccharomyces pombe rad1+ checkpoint gene encodes an exonuclease. J Biol Chem 273(29):18332-9
2)	Lydall D, et al. (1996) A meiotic recombination checkpoint controlled by mitotic checkpoint genes. Nature 383(6603):840-3
3)	Majka J and Burgers PM (2003) Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint. Proc Natl Acad Sci U S A 100(5):2249-54
4)	Shinohara M, et al. (2003) The mitotic DNA damage checkpoint proteins Rad17 and Rad24 are required for repair of double-strand breaks during meiosis in yeast. Genetics 164(3):855-65
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41