PRT1/YOR361C Protein Information Help

Standard Name Prt1p
Systematic Name Yor361cp
Alias Cdc63p 1 , Dna26p 2
ORF Classification Verified
Description eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes (3, 4, 5, 6)
Name Description PRoTein synthesis
Experimental Data
Molecules/cell 47500 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 763
Molecular Weight (Da) 88,129
Isoelectric Point (pI) 5.88

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Prt1p (InterPro)
Physical Interactions There are 241 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052ECE | P06103
MIPS: YOR361C
NCBI: 115936 | 1420784 | 172286 | 308198609 | 308198610 | 308198611 | 308198612 | 349587938 | 349587939 | 398366379 | NP_015006.3
GenBank/EMBL/DDBJ: DAA11122.1 | J02674 | Z75269
Amino Acid Sequence (or in FASTA format)
 
       1  MKNFLPRTLK NIYELYFNNI SVHSIVSRNT QLKRSKIIQM TTETFEDIKL
      51  EDIPVDDIDF SDLEEQYKVT EEFNFDQYIV VNGAPVIPSA KVPVLKKALT
     101  SLFSKAGKVV NMEFPIDEAT GKTKGFLFVE CGSMNDAKKI IKSFHGKRLD
     151  LKHRLFLYTM KDVERYNSDD FDTEFREPDM PTFVPSSSLK SWLMDDKVRD
     201  QFVLQDDVKT SVFWNSMFNE EDSLVESREN WSTNYVRFSP KGTYLFSYHQ
     251  QGVTAWGGPN FDRLRRFYHP DVRNSSVSPN EKYLVTFSTE PIIVEEDNEF
     301  SPFTKKNEGH QLCIWDIASG LLMATFPVIK SPYLKWPLVR WSYNDKYCAR
     351  MVGDSLIVHD ATKNFMPLEA KALKPSGIRD FSFAPEGVKL QPFRNGDEPS
     401  VLLAYWTPET NNSACTATIA EVPRGRVLKT VNLVQVSNVT LHWQNQAEFL
     451  CFNVERHTKS GKTQFSNLQI CRLTERDIPV EKVELKDSVF EFGWEPHGNR
     501  FVTISVHEVA DMNYAIPANT IRFYAPETKE KTDVIKRWSL VKEIPKTFAN
     551  TVSWSPAGRF VVVGALVGPN MRRSDLQFYD MDYPGEKNIN DNNDVSASLK
     601  DVAHPTYSAA TNITWDPSGR YVTAWSSSLK HKVEHGYKIF NIAGNLVKED
     651  IIAGFKNFAW RPRPASILSN AERKKVRKNL REWSAQFEEQ DAMEADTAMR
     701  DLILHQRELL KQWTEYREKI GQEMEKSMNF KIFDVQPEDA SDDFTTIEEI
     751  VEEVLEETKE KVE*

external links for Prt1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome

YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Prt1p
1) Bedard DP, et al.  (1981) New mutations in the yeast Saccharomyces cerevisiae affecting completion of Start. Curr Genet 4:205-214
2) Dumas LB, et al.  (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol Gen Genet 187(1):42-6
3) Phan L, et al.  (1998) Identification of a translation initiation factor 3 (eIF3) core complex, conserved in yeast and mammals, that interacts with eIF5. Mol Cell Biol 18(8):4935-46
4) Naranda T, et al.  (1994) Purified yeast translational initiation factor eIF-3 is an RNA-binding protein complex that contains the PRT1 protein. J Biol Chem 269(51):32286-92
5) Keierleber C, et al.  (1986) Isolation and characterization of PRT1, a gene required for the initiation of protein biosynthesis in Saccharomyces cerevisiae. Mol Cell Biol 6(12):4419-24
6) Phan L, et al.  (2001) A subcomplex of three eIF3 subunits binds eIF1 and eIF5 and stimulates ribosome binding of mRNA and tRNA(i)Met. EMBO J 20(11):2954-65
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41