PDE2/YOR360C Protein Information Help

Standard Name Pde2p 1
Systematic Name Yor360cp
Alias Sra5p 2
ORF Classification Verified
Description High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon (1, 3, 4, 5)
Name Description PhosphoDiEsterase 6
Experimental Data
Molecules/cell 6510 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 526
Molecular Weight (Da) 60,999
Isoelectric Point (pI) 6.6

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pde2p (InterPro)
Physical Interactions There are 6 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000127C07 | P06776
MIPS: YOR360C
NCBI: 1420782 | 172106 | 2507163 | 6324936 | NP_015005.1
GenBank/EMBL/DDBJ: DAA11121.1 | M14563 | Z75268
External Classifications EC: 3.1.4.17 [3',5'-cyclic-nucleotide phosphodiesterase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSTLFLIGIH EIEKSQTIVQ NEHYFDRVIE LQDLDSLMVA LYKDRVSPFP
      51  NVHNFETGVS IVLYDPSKFQ LSVRQLDVLF KRFFPSFNIS AIDHTREENL
     101  QRLECVEREN SICRNRITRI NHWMYHHHDD TPDGINKNSY GTVNGNSVPT
     151  QACEANIYTL LLHLNDSKAQ HLRKASVPRL IRNIEFMSFL SDPIEKISQE
     201  GSHYWNILST WDFCALSLST QELIWCGFTL IKKLSKDAKV LIADNKLLLL
     251  LFTLESSYHQ VNKFHNFRHA IDVMQATWRL CTYLLKDNPV QTLLLCMAAI
     301  GHDVGHPGTN NQLLCNCESE VAQNFKNVSI LENFHRELFQ QLLSEHWPQL
     351  LSISKKKFDF ISEAILATDM ALHSQYEDRL MHENPMKQIT LISLIIKAAD
     401  ISNVTRTLSI SARWAYLITL EFNDCALLET FHKAHRPEQD CFGDSYKNVD
     451  SPKEDLESIQ NILVNVTDPD DIIKDHPHIP NGQIFFINTF AEVFFNALSQ
     501  KFSGLKFLSD NVKINKEYWM KHKKPQ*

external links for Pde2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Pde2p
1) Sass P, et al.  (1986) Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 83(24):9303-7
2) Wilson RB and Tatchell K  (1988) SRA5 encodes the low-Km cyclic AMP phosphodiesterase of Saccharomyces cerevisiae. Mol Cell Biol 8(1):505-10
3) Nikawa J, et al.  (1987) Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae. Mol Cell Biol 7(10):3629-36
4) Wilson RB, et al.  (1993) The pde2 gene of Saccharomyces cerevisiae is allelic to rca1 and encodes a phosphodiesterase which protects the cell from extracellular cAMP. FEBS Lett 325(3):191-5
5) Namy O, et al.  (2002) Translational readthrough of the PDE2 stop codon modulates cAMP levels in Saccharomyces cerevisiae. Mol Microbiol 43(3):641-52
6) Uno I, et al.  (1983) Characterization of a cyclic nucleotide phosphodiesterase-deficient mutant in yeast. J Biol Chem 258(6):3539-42
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41