PUT4/YOR348C Protein Information

Standard Name	Put4p
Systematic Name	Yor348cp
ORF Classification	Verified
Description	Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells (1, 2)
Name Description	Proline UTilization

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	627
Molecular Weight (Da)	68,787
Isoelectric Point (pI)	7.47

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Put4p (InterPro)
Transmembrane Domains	There are 11 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 5 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000132B80 \| P15380 MIPS: YOR348C NCBI: 1199850 \| 1420758 \| 1709941 \| 172307 \| 6324924 \| NP_014993.1 \| NM_001183768.1 GenBank/EMBL/DDBJ: DAA11109.1 \| M30583 \| X95720 \| Z75256
External Classifications	TC: 2.A.3.10 [The Yeast Amino Acid Transporter (YAT) Family]

Amino Acid Sequence (or in FASTA format)
1 MVNILPFHKN NRHSAGVVTC ADDVSGDGSG GDTKKEEDVV QVTESPSSGS 51 RNNHRSDNEK DDAIRMEKIS KNQSASSNGT IREDLIMDVD LEKSPSVDGD 101 SEPHKLKQGL QSRHVQLIAL GGAIGTGLLV GTSSTLHTCG PAGLFISYII 151 ISAVIYPIMC ALGEMVCFLP GDGSDSAGST ANLVTRYVDP SLGFATGWNY 201 FYCYVILVAA ECTAASGVVE YWTTAVPKGV WITIFLCVVV ILNFSAVKVY 251 GESEFWFASI KILCIVGLII LSFILFWGGG PNHDRLGFRY WQHPGAFAHH 301 LTGGSLGNFT DIYTGIIKGA FAFILGPELV CMTSAECADQ RRNIAKASRR 351 FVWRLIFFYV LGTLAISVIV PYNDPTLVNA LAQGKPGAGS SPFVIGIQNA 401 GIKVLPHIIN GCILTSAWSA ANAFMFASTR SLLTMAQTGQ APKCLGRINK 451 WGVPYVAVGV SFLCSCLAYL NVSSSTADVF NWFSNISTIS GFLGWMCGCI 501 AYLRFRKAIF YNGLYDRLPF KTWGQPYTVW FSLIVIGIIT ITNGYAIFIP 551 KYWRVADFIA AYITLPIFLV LWFGHKLYTR TWRQWWLPVS EIDVTTGLVE 601 IEEKSREIEE MRLPPTGFKD KFLDALL*

Amino Acid Sequence (or in FASTA format)

       1  MVNILPFHKN NRHSAGVVTC ADDVSGDGSG GDTKKEEDVV QVTESPSSGS
      51  RNNHRSDNEK DDAIRMEKIS KNQSASSNGT IREDLIMDVD LEKSPSVDGD
     101  SEPHKLKQGL QSRHVQLIAL GGAIGTGLLV GTSSTLHTCG PAGLFISYII
     151  ISAVIYPIMC ALGEMVCFLP GDGSDSAGST ANLVTRYVDP SLGFATGWNY
     201  FYCYVILVAA ECTAASGVVE YWTTAVPKGV WITIFLCVVV ILNFSAVKVY
     251  GESEFWFASI KILCIVGLII LSFILFWGGG PNHDRLGFRY WQHPGAFAHH
     301  LTGGSLGNFT DIYTGIIKGA FAFILGPELV CMTSAECADQ RRNIAKASRR
     351  FVWRLIFFYV LGTLAISVIV PYNDPTLVNA LAQGKPGAGS SPFVIGIQNA
     401  GIKVLPHIIN GCILTSAWSA ANAFMFASTR SLLTMAQTGQ APKCLGRINK
     451  WGVPYVAVGV SFLCSCLAYL NVSSSTADVF NWFSNISTIS GFLGWMCGCI
     501  AYLRFRKAIF YNGLYDRLPF KTWGQPYTVW FSLIVIGIIT ITNGYAIFIP
     551  KYWRVADFIA AYITLPIFLV LWFGHKLYTR TWRQWWLPVS EIDVTTGLVE
     601  IEEKSREIEE MRLPPTGFKD KFLDALL*

external links for Put4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	TCDB
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Put4p

1)	Jauniaux JC, et al. (1987) Nitrogen catabolite regulation of proline permease in Saccharomyces cerevisiae. Cloning of the PUT4 gene and study of PUT4 RNA levels in wild-type and mutant strains. Eur J Biochem 164(3):601-6
2)	Andreasson C, et al. (2004) Four permeases import proline and the toxic proline analogue azetidine-2-carboxylate into yeast. Yeast 21(3):193-9