PYK2/YOR347C Protein Information

Standard Name	Pyk2p
Systematic Name	Yor347cp
ORF Classification	Verified
Description	Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication (1, 2, 3)
Name Description	PYruvate Kinase

Experimental Data
Molecules/cell	2130 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	506
Molecular Weight (Da)	55,195
Isoelectric Point (pI)	6.9

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pyk2p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012DFFD \| P52489 MIPS: YOR347C NCBI: 1199849 \| 1420756 \| 1730063 \| 398366329 \| NP_014992.3 \| NM_001183767.3 GenBank/EMBL/DDBJ: DAA11108.1 \| X95720 \| Z75255
External Classifications	EC: 2.7.1.40 [Pyruvate kinase]

Amino Acid Sequence (or in FASTA format)
1 MPESRLQRLA NLKIGTPQQL RRTSIIGTIG PKTNSCEAIT ALRKAGLNII 51 RLNFSHGSYE FHQSVIENAV KSEQQFPGRP LAIALDTKGP EIRTGRTLND 101 QDLYIPVDHQ MIFTTDASFA NTSNDKIMYI DYANLTKVIV PGRFIYVDDG 151 ILSFKVLQII DESNLRVQAV NSGYIASHKG VNLPNTDVDL PPLSAKDMKD 201 LQFGVRNGIH IVFASFIRTS EDVLSIRKAL GSEGQDIKII SKIENQQGLD 251 NFDEILEVTD GVMIARGDLG IEILAPEVLA IQKKLIAKCN LAGKPVICAT 301 QMLDSMTHNP RPTRAEVSDV GNAVLDGADC VMLSGETAKG DYPVNAVNIM 351 AATALIAEST IAHLALYDDL RDATPKPTST TETVAAAATA AILEQDGKAI 401 VVLSTTGNTA RLLSKYRPSC PIILVTRHAR TARIAHLYRG VFPFLYEPKR 451 LDDWGEDVHR RLKFGVEMAR SFGMVDNGDT VVSIQGFKGG VGHSNTLRIS 501 TVGQEF*

Amino Acid Sequence (or in FASTA format)

       1  MPESRLQRLA NLKIGTPQQL RRTSIIGTIG PKTNSCEAIT ALRKAGLNII
      51  RLNFSHGSYE FHQSVIENAV KSEQQFPGRP LAIALDTKGP EIRTGRTLND
     101  QDLYIPVDHQ MIFTTDASFA NTSNDKIMYI DYANLTKVIV PGRFIYVDDG
     151  ILSFKVLQII DESNLRVQAV NSGYIASHKG VNLPNTDVDL PPLSAKDMKD
     201  LQFGVRNGIH IVFASFIRTS EDVLSIRKAL GSEGQDIKII SKIENQQGLD
     251  NFDEILEVTD GVMIARGDLG IEILAPEVLA IQKKLIAKCN LAGKPVICAT
     301  QMLDSMTHNP RPTRAEVSDV GNAVLDGADC VMLSGETAKG DYPVNAVNIM
     351  AATALIAEST IAHLALYDDL RDATPKPTST TETVAAAATA AILEQDGKAI
     401  VVLSTTGNTA RLLSKYRPSC PIILVTRHAR TARIAHLYRG VFPFLYEPKR
     451  LDDWGEDVHR RLKFGVEMAR SFGMVDNGDT VVSIQGFKGG VGHSNTLRIS
     501  TVGQEF*

external links for Pyk2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Pyk2p

1)	Boles E, et al. (1997) Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate. J Bacteriol 179(9):2987-93
2)	Portela P, et al. (2002) In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A. J Biol Chem 277(34):30477-87
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41