RPA190/YOR341W Protein Information

Standard Name	Rpa190p 1
Systematic Name	Yor341wp
Alias	Rrn1p 2
ORF Classification	Verified
Description	RNA polymerase I largest subunit A190 (1, 3)
Name Description	RNA Polymerase A
Gene Product	A190 4

Experimental Data
Molecules/cell	2840 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,664
Molecular Weight (Da)	186,430
Isoelectric Point (pI)	7.05

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rpa190p (InterPro)
Physical Interactions	There are 185 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053185 \| P10964 MIPS: YOR341W NCBI: 1199844 \| 1420741 \| 172215 \| 2507346 \| 398366307 \| NP_014986.3 GenBank/EMBL/DDBJ: DAA11102.1 \| J03530 \| X95720 \| Z75249
External Classifications	EC: 2.7.7.6 [DNA-directed RNA polymerase]

Amino Acid Sequence (or in FASTA format)
1 MDISKPVGSE ITSVDFGILT AKEIRNLSAK QITNPTVLDN LGHPVSGGLY 51 DLALGAFLRN LCSTCGLDEK FCPGHQGHIE LPVPCYNPLF FNQLYIYLRA 101 SCLFCHHFRL KSVEVHRYAC KLRLLQYGLI DESYKLDEIT LGSLNSSMYT 151 DDEAIEDNED EMDGEGSKQS KDISSTLLNE LKSKRSEYVD MAIAKALSDG 201 RTTERGSFTA TVNDERKKLV HEFHKKLLSR GKCDNCGMFS PKFRKDGFTK 251 IFETALNEKQ ITNNRVKGFI RQDMIKKQKQ AKKLDGSNEA SANDEESFDV 301 GRNPTTRPKT GSTYILSTEV KNILDTVFRK EQCVLQYVFH SRPNLSRKLV 351 KADSFFMDVL VVPPTRFRLP SKLGEEVHEN SQNQLLSKVL TTSLLIRDLN 401 DDLSKLQKDK VSLEDRRVIF SRLMNAFVTI QNDVNAFIDS TKAQGRTSGK 451 VPIPGVKQAL EKKEGLFRKH MMGKRVNYAA RSVISPDPNI ETNEIGVPPV 501 FAVKLTYPEP VTAYNIAELR QAVINGPDKW PGATQIQNED GSLVSLIGMS 551 VEQRKALANQ LLTPSSNVST HTLNKKVYRH IKNRDVVLMN RQPTLHKASM 601 MGHKVRVLPN EKTLRLHYAN TGAYNADFDG DEMNMHFPQN ENARAEALNL 651 ANTDSQYLTP TSGSPVRGLI QDHISAGVWL TSKDSFFTRE QYQQYIYGCI 701 RPEDGHTTRS KIVTLPPTIF KPYPLWTGKQ IITTVLLNVT PPDMPGINLI 751 SKNKIKNEYW GKGSLENEVL FKDGALLCGI LDKSQYGASK YGIVHSLHEV 801 YGPEVAAKVL SVLGRLFTNY ITATAFTCGM DDLRLTAEGN KWRTDILKTS 851 VDTGREAAAE VTNLDKDTPA DDPELLKRLQ EILRDNNKSG ILDAVTSSKV 901 NAITSQVVSK CVPDGTMKKF PCNSMQAMAL SGAKGSNVNV SQIMCLLGQQ 951 ALEGRRVPVM VSGKTLPSFK PYETDAMAGG YVKGRFYSGI KPQEYYFHCM 1001 AGREGLIDTA VKTSRSGYLQ RCLTKQLEGV HVSYDNSIRD ADGTLVQFMY 1051 GGDAIDITKE SHMTQFEFCL DNYYALLKKY NPSALIEHLD VESALKYSKK 1101 TLKYRKKHSK EPHYKQSVKY DPVLAKYNPA KYLGSVSENF QDKLESFLDK 1151 NSKLFKSSDG VNEKKFRALM QLKYMRSLIN PGEAVGIIAS QSVGEPSTQM 1201 TLNTFHFAGH GAANVTLGIP RLREIVMTAS AAIKTPQMTL PIWNDVSDEQ 1251 ADTFCKSISK VLLSEVIDKV IVTETTGTSN TAGGNAARSY VIHMRFFDNN 1301 EYSEEYDVSK EELQNVISNQ FIHLLEAAIV KEIKKQKRTT GPDIGVAVPR 1351 LQTDVANSSS NSKRLEEDND EEQSHKKTKQ AVSYDEPDED EIETMREAEK 1401 SSDEEGIDSD KESDSDSEDE DVDMNEQINK SIVEANNNMN KVQRDRQSAI 1451 ISHHRFITKY NFDDESGKWC EFKLELAADT EKLLMVNIVE EICRKSIIRQ 1501 IPHIDRCVHP EPENGKRVLV TEGVNFQAMW DQEAFIDVDG ITSNDVAAVL 1551 KTYGVEAARN TIVNEINNVF SRYAISVSFR HLDLIADMMT RQGTYLAFNR 1601 QGMETSTSSF MKMSYETTCQ FLTKAVLDNE REQLDSPSAR IVVGKLNNVG 1651 TGSFDVLAKV PNAA*

Amino Acid Sequence (or in FASTA format)

       1  MDISKPVGSE ITSVDFGILT AKEIRNLSAK QITNPTVLDN LGHPVSGGLY
      51  DLALGAFLRN LCSTCGLDEK FCPGHQGHIE LPVPCYNPLF FNQLYIYLRA
     101  SCLFCHHFRL KSVEVHRYAC KLRLLQYGLI DESYKLDEIT LGSLNSSMYT
     151  DDEAIEDNED EMDGEGSKQS KDISSTLLNE LKSKRSEYVD MAIAKALSDG
     201  RTTERGSFTA TVNDERKKLV HEFHKKLLSR GKCDNCGMFS PKFRKDGFTK
     251  IFETALNEKQ ITNNRVKGFI RQDMIKKQKQ AKKLDGSNEA SANDEESFDV
     301  GRNPTTRPKT GSTYILSTEV KNILDTVFRK EQCVLQYVFH SRPNLSRKLV
     351  KADSFFMDVL VVPPTRFRLP SKLGEEVHEN SQNQLLSKVL TTSLLIRDLN
     401  DDLSKLQKDK VSLEDRRVIF SRLMNAFVTI QNDVNAFIDS TKAQGRTSGK
     451  VPIPGVKQAL EKKEGLFRKH MMGKRVNYAA RSVISPDPNI ETNEIGVPPV
     501  FAVKLTYPEP VTAYNIAELR QAVINGPDKW PGATQIQNED GSLVSLIGMS
     551  VEQRKALANQ LLTPSSNVST HTLNKKVYRH IKNRDVVLMN RQPTLHKASM
     601  MGHKVRVLPN EKTLRLHYAN TGAYNADFDG DEMNMHFPQN ENARAEALNL
     651  ANTDSQYLTP TSGSPVRGLI QDHISAGVWL TSKDSFFTRE QYQQYIYGCI
     701  RPEDGHTTRS KIVTLPPTIF KPYPLWTGKQ IITTVLLNVT PPDMPGINLI
     751  SKNKIKNEYW GKGSLENEVL FKDGALLCGI LDKSQYGASK YGIVHSLHEV
     801  YGPEVAAKVL SVLGRLFTNY ITATAFTCGM DDLRLTAEGN KWRTDILKTS
     851  VDTGREAAAE VTNLDKDTPA DDPELLKRLQ EILRDNNKSG ILDAVTSSKV
     901  NAITSQVVSK CVPDGTMKKF PCNSMQAMAL SGAKGSNVNV SQIMCLLGQQ
     951  ALEGRRVPVM VSGKTLPSFK PYETDAMAGG YVKGRFYSGI KPQEYYFHCM
    1001  AGREGLIDTA VKTSRSGYLQ RCLTKQLEGV HVSYDNSIRD ADGTLVQFMY
    1051  GGDAIDITKE SHMTQFEFCL DNYYALLKKY NPSALIEHLD VESALKYSKK
    1101  TLKYRKKHSK EPHYKQSVKY DPVLAKYNPA KYLGSVSENF QDKLESFLDK
    1151  NSKLFKSSDG VNEKKFRALM QLKYMRSLIN PGEAVGIIAS QSVGEPSTQM
    1201  TLNTFHFAGH GAANVTLGIP RLREIVMTAS AAIKTPQMTL PIWNDVSDEQ
    1251  ADTFCKSISK VLLSEVIDKV IVTETTGTSN TAGGNAARSY VIHMRFFDNN
    1301  EYSEEYDVSK EELQNVISNQ FIHLLEAAIV KEIKKQKRTT GPDIGVAVPR
    1351  LQTDVANSSS NSKRLEEDND EEQSHKKTKQ AVSYDEPDED EIETMREAEK
    1401  SSDEEGIDSD KESDSDSEDE DVDMNEQINK SIVEANNNMN KVQRDRQSAI
    1451  ISHHRFITKY NFDDESGKWC EFKLELAADT EKLLMVNIVE EICRKSIIRQ
    1501  IPHIDRCVHP EPENGKRVLV TEGVNFQAMW DQEAFIDVDG ITSNDVAAVL
    1551  KTYGVEAARN TIVNEINNVF SRYAISVSFR HLDLIADMMT RQGTYLAFNR
    1601  QGMETSTSSF MKMSYETTCQ FLTKAVLDNE REQLDSPSAR IVVGKLNNVG
    1651  TGSFDVLAKV PNAA*

external links for Rpa190p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rpa190p

1)	Memet S, et al. (1988) RPA190, the gene coding for the largest subunit of yeast RNA polymerase A. J Biol Chem 263(6):2830-9
2)	Nogi Y, et al. (1991) An approach for isolation of mutants defective in 35S ribosomal RNA synthesis in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 88(16):7026-30
3)	Wittekind M, et al. (1988) Isolation and characterization of temperature-sensitive mutations in RPA190, the gene encoding the largest subunit of RNA polymerase I from Saccharomyces cerevisiae. Mol Cell Biol 8(10):3997-4008
4)	Riva M, et al. (1982) Natural variation in yeast RNA polymerase A. Formation of a mosaic RNA polymerase A in a meiotic segregant from an interspecific hybrid. J Biol Chem 257(8):4570-7
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41