ALA1/YOR335C Protein Information Help

Standard Name Ala1p
Systematic Name Yor335cp
Alias Cdc64p 1
ORF Classification Verified
Description Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog (2, 3, 4)
Name Description ALAnyl-tRNA synthetase 3
Experimental Data
Molecules/cell 33800 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 958
Molecular Weight (Da) 107,276
Isoelectric Point (pI) 5.27

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ala1p (InterPro)
Physical Interactions There are 35 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0001E88E74 | P40825
MIPS: YOR335C
NCBI: 1163069 | 1420729 | 1711623 | 313104284 | 398366285 | 609338 | NP_014980.3
GenBank/EMBL/DDBJ: DAA11096.1 | U18672 | Z49821 | Z75243
External Classifications EC: 6.1.1.7 [Alanine--tRNA ligase]
Amino Acid Sequence (or in FASTA format)
 
       1  MTIGDKQKWT ATNVRNTFLD YFKSKEHKFV KSSPVVPFDD PTLLFANAGM
      51  NQYKPIFLGT VDPASDFYTL KRAYNSQKCI RAGGKHNDLE DVGKDSYHHT
     101  FFEMLGNWSF GDYFKKEAIT YSWTLLTEVY GIPKDRLYVT YFEGDEKLGL
     151  EPDTEARELW KNVGVPDDHI LPGNAKDNFW EMGDQGPCGP CSEIHYDRIG
     201  GRNAASLVNM DDPDVLEVWN LVFIQFNREQ DGSLKPLPAK HIDTGMGFER
     251  LVSVLQDVRS NYDTDVFTPL FERIQEITSV RPYSGNFGEN DKDGIDTAYR
     301  VLADHVRTLT FALADGGVPN NEGRGYVLRR ILRRGARYAR KYMNYPIGNF
     351  FSTLAPTLIS QVQDIFPELA KDPAFLFEIL DEEEASFAKT LDRGERLFEK
     401  YASAASKTES KTLDGKQVWR LYDTYGFPVD LTELMAEEQG LKIDGPGFEK
     451  AKQESYEASK RGGKKDQSDL IKLNVHELSE LNDAKVPKTN DEFKYGSANV
     501  EGTILKLHDG TNFVDEITEP GKKYGIILDK TCFYAEQGGQ EYDTGKIVID
     551  DAAEFNVENV QLYNGFVFHT GSLEEGKLSV GDKIIASFDE LRRFPIKNNH
     601  TGTHILNFAL KETLGNDVDQ KGSLVAPEKL RFDFSHKKAV SNEELKKVED
     651  ICNEQIKENL QVFYKEIPLD LAKSIDGVRA VFGETYPDPV RVVSVGKPIE
     701  ELLANPANEE WTKYSIEFCG GTHVNKTGDI KYFVILEESG IAKGIRRIVA
     751  VTGTEAFEAQ RLAEQFAADL DAADKLPFSP IKEKKLKELG VKLGQLSISV
     801  ITKNELKQKF NKIEKAVKDE VKSRAKKENK QTLDEVKTFF ETNENAPYLV
     851  KFIDISPNAK AITEAINYMK SNDSVKDKSI YLLAGNDPEG RVAHGCYISN
     901  AALAKGIDGS ALAKKVSSII GGKAGGKGNV FQGMGDKPAA IKDAVDDLES
     951  LFKEKLSI*

external links for Ala1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Ala1p
1) Bedard DP, et al.  (1981) New mutations in the yeast Saccharomyces cerevisiae affecting completion of Start. Curr Genet 4:205-214
2) Wrobel C, et al.  (1999) CDC64 encodes cytoplasmic alanyl-tRNA synthetase, Ala1p, of Saccharomyces cerevisiae. J Bacteriol 181(24):7618-20
3) Ripmaster TL, et al.  (1995) Wide cross-species aminoacyl-tRNA synthetase replacement in vivo: yeast cytoplasmic alanine enzyme replaced by human polymyositis serum antigen. Proc Natl Acad Sci U S A 92(11):4932-6
4) Tang HL, et al.  (2004) Translation of a yeast mitochondrial tRNA synthetase initiated at redundant non-AUG codons. J Biol Chem 279(48):49656-63
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41