MIP1/YOR330C Protein Information

Standard Name	Mip1p 1
Systematic Name	Yor330cp
ORF Classification	Verified
Description	Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit (1, 2, 3, 4, 5, 6)
Name Description	MItochondrial DNA Polymerase
Gene Product	mitochondrial DNA polymerase catalytic subunit 1

Experimental Data
Molecules/cell	377 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,254
Molecular Weight (Da)	143,501
Isoelectric Point (pI)	9.1

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mip1p (InterPro)
Physical Interactions	There are 9 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001297CD \| P15801 MIPS: YOR330C NCBI: 1147767 \| 1163066 \| 1420721 \| 1706509 \| 470326 \| 83578108 \| NP_014975.2 \| NM_001183750.1 GenBank/EMBL/DDBJ: DAA11093.1 \| J05117 \| U42227 \| Z49821 \| Z75238
External Classifications	EC: 2.7.7.7 [DNA-directed DNA polymerase]

Amino Acid Sequence (or in FASTA format)
1 MTKLMVRSEC MLRMVRRRPL RVQFCARWFS TKKNTAEAPR INPVGIQYLG 51 ESLQRQVFGS CGGKDEVEQS DKLMELSKKS LKDHGLWGKK TLITDPISFP 101 LPPLQGRSLD EHFQKIGRFN SEPYKSFCED KFTEMVARPA EWLRKPGWVK 151 YVPGMAPVEV AYPDEELVVF DVETLYNVSD YPTLATALSS TAWYLWCSPF 201 ICGGDDPAAL IPLNTLNKEQ VIIGHNVAYD RARVLEEYNF RDSKAFFLDT 251 QSLHIASFGL CSRQRPMFMK NNKKKEAEVE SEVHPEISIE DYDDPWLNVS 301 ALNSLKDVAK FHCKIDLDKT DRDFFASTDK STIIENFQKL VNYCATDVTA 351 TSQVFDEIFP VFLKKCPHPV SFAGLKSLSK CILPTKLNDW NDYLNSSESL 401 YQQSKVQIES KIVQIIKDIV LLKDKPDFYL KDPWLSQLDW TTKPLRLTKK 451 GVPAKCQKLP GFPEWYRQLF PSKDTVEPKI TIKSRIIPIL FKLSWENSPV 501 IWSKESGWCF NVPHEQVETY KAKNYVLADS VSQEEEEIRT HNLGLQCTGV 551 LFKVPHPNGP TFNCTNLLTK SYNHFFEKGV LKSESELAHQ ALQINSSGSY 601 WMSARERIQS QFVVPSCKFP NEFQSLSAKS SLNNEKTNDL AIIIPKIVPM 651 GTITRRAVEN AWLTASNAKA NRIGSELKTQ VKAPPGYCFV GADVDSEELW 701 IASLVGDSIF NVHGGTAIGW MCLEGTKNEG TDLHTKTAQI LGCSRNEAKI 751 FNYGRIYGAG AKFASQLLKR FNPSLTDEET KKIANKLYEN TKGKTKRSKL 801 FKKFWYGGSE SILFNKLESI AEQETPKTPV LGCGITYSLM KKNLRANSFL 851 PSRINWAIQS SGVDYLHLLC CSMEYIIKKY NLEARLCISI HDEIRFLVSE 901 KDKYRAAMAL QISNIWTRAM FCQQMGINEL PQNCAFFSQV DIDSVIRKEV 951 NMDCITPSNK TAIPHGEALD INQLLDKSNS KLGKPNLDID SKVSQYAYNY 1001 REPVFEEYNK SYTPEFLKYF LAMQVQSDKR DVNRLEDEYL RECTSKEYAR 1051 DGNTAEYSLL DYIKDVEKGK RTKVRIMGSN FLDGTKNAKA DQRIRLPVNM 1101 PDYPTLHKIA NDSAIPEKQL LENRRKKENR IDDENKKKLT RKKNTTPMER 1151 KYKRVYGGRK AFEAFYECAN KPLDYTLETE KQFFNIPIDG VIDDVLNDKS 1201 NYKKKPSQAR TASSSPIRKT AKAVHSKKLP ARKSSTTNRN LVELERDITI 1251 SREY*

Amino Acid Sequence (or in FASTA format)

       1  MTKLMVRSEC MLRMVRRRPL RVQFCARWFS TKKNTAEAPR INPVGIQYLG
      51  ESLQRQVFGS CGGKDEVEQS DKLMELSKKS LKDHGLWGKK TLITDPISFP
     101  LPPLQGRSLD EHFQKIGRFN SEPYKSFCED KFTEMVARPA EWLRKPGWVK
     151  YVPGMAPVEV AYPDEELVVF DVETLYNVSD YPTLATALSS TAWYLWCSPF
     201  ICGGDDPAAL IPLNTLNKEQ VIIGHNVAYD RARVLEEYNF RDSKAFFLDT
     251  QSLHIASFGL CSRQRPMFMK NNKKKEAEVE SEVHPEISIE DYDDPWLNVS
     301  ALNSLKDVAK FHCKIDLDKT DRDFFASTDK STIIENFQKL VNYCATDVTA
     351  TSQVFDEIFP VFLKKCPHPV SFAGLKSLSK CILPTKLNDW NDYLNSSESL
     401  YQQSKVQIES KIVQIIKDIV LLKDKPDFYL KDPWLSQLDW TTKPLRLTKK
     451  GVPAKCQKLP GFPEWYRQLF PSKDTVEPKI TIKSRIIPIL FKLSWENSPV
     501  IWSKESGWCF NVPHEQVETY KAKNYVLADS VSQEEEEIRT HNLGLQCTGV
     551  LFKVPHPNGP TFNCTNLLTK SYNHFFEKGV LKSESELAHQ ALQINSSGSY
     601  WMSARERIQS QFVVPSCKFP NEFQSLSAKS SLNNEKTNDL AIIIPKIVPM
     651  GTITRRAVEN AWLTASNAKA NRIGSELKTQ VKAPPGYCFV GADVDSEELW
     701  IASLVGDSIF NVHGGTAIGW MCLEGTKNEG TDLHTKTAQI LGCSRNEAKI
     751  FNYGRIYGAG AKFASQLLKR FNPSLTDEET KKIANKLYEN TKGKTKRSKL
     801  FKKFWYGGSE SILFNKLESI AEQETPKTPV LGCGITYSLM KKNLRANSFL
     851  PSRINWAIQS SGVDYLHLLC CSMEYIIKKY NLEARLCISI HDEIRFLVSE
     901  KDKYRAAMAL QISNIWTRAM FCQQMGINEL PQNCAFFSQV DIDSVIRKEV
     951  NMDCITPSNK TAIPHGEALD INQLLDKSNS KLGKPNLDID SKVSQYAYNY
    1001  REPVFEEYNK SYTPEFLKYF LAMQVQSDKR DVNRLEDEYL RECTSKEYAR
    1051  DGNTAEYSLL DYIKDVEKGK RTKVRIMGSN FLDGTKNAKA DQRIRLPVNM
    1101  PDYPTLHKIA NDSAIPEKQL LENRRKKENR IDDENKKKLT RKKNTTPMER
    1151  KYKRVYGGRK AFEAFYECAN KPLDYTLETE KQFFNIPIDG VIDDVLNDKS
    1201  NYKKKPSQAR TASSSPIRKT AKAVHSKKLP ARKSSTTNRN LVELERDITI
    1251  SREY*

external links for Mip1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Mip1p

1)	Foury F (1989) Cloning and sequencing of the nuclear gene MIP1 encoding the catalytic subunit of the yeast mitochondrial DNA polymerase. J Biol Chem 264(34):20552-60
2)	Lucas P, et al. (2004) Absence of accessory subunit in the DNA polymerase gamma purified from yeast mitochondria. Mitochondrion 4(1):13-20
3)	Stuart GR, et al. (2006) Mitochondrial and nuclear DNA defects in Saccharomyces cerevisiae with mutations in DNA polymerase gamma associated with progressive external ophthalmoplegia. Hum Mol Genet 15(2):363-74
4)	Young MJ, et al. (2006) The carboxyl-terminal extension on fungal mitochondrial DNA polymerases: identification of a critical region of the enzyme from Saccharomyces cerevisiae. Yeast 23(2):101-16
5)	Baruffini E, et al. (2011) Predicting the contribution of novel POLG mutations to human disease through analysis in yeast model. Mitochondrion 11(1):182-90
6)	Stewart JD, et al. (2010) Polymerase ? gene POLG determines the risk of sodium valproate-induced liver toxicity. Hepatology 52(5):1791-6
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41