DGK1/YOR311C Protein Information

Standard Name	Dgk1p 1, 2
Systematic Name	Yor311cp
Alias	Hsd1p 3
ORF Classification	Verified
Description	Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain (1, 2, 3)
Name Description	DiacylGlycerol Kinase 1, 2

Experimental Data
Molecules/cell	784 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	290
Molecular Weight (Da)	32,840
Isoelectric Point (pI)	9.48

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Dgk1p (InterPro)
Transmembrane Domains	There are 4 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 6 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006BB14 \| Q12382 MIPS: YOR311C NCBI: 1420684 \| 398366205 \| 74583839 \| 940842 \| NP_014956.3 GenBank/EMBL/DDBJ: DAA11076.1 \| X90565 \| Z75219

Amino Acid Sequence (or in FASTA format)
1 MGTEDAIALP NSTLEPRTEA KQRLSSKSHQ VSAKVTIPAK EEISSSDDDA 51 HVPVTEIHLK SHEWFGDFIT KHEIPRKVFH SSIGFITLYL YTQGINYKNV 101 LWPLIYAFII LFILDLIRLN WPFFNMLYCR TVGALMRKKE IHTYNGVLWY 151 ILGLIFSFNF FSKDVTLISL FLLSWSDTAA ATIGRKYGHL TPKVARNKSL 201 AGSIAAFTVG VITCWVFYGY FVPAYSYVNK PGEIQWSPET SRLSLNMLSL 251 LGGVVAALSE GIDLFNWDDN FTIPVLSSLF MNAVIKTFKK *

Amino Acid Sequence (or in FASTA format)

       1  MGTEDAIALP NSTLEPRTEA KQRLSSKSHQ VSAKVTIPAK EEISSSDDDA
      51  HVPVTEIHLK SHEWFGDFIT KHEIPRKVFH SSIGFITLYL YTQGINYKNV
     101  LWPLIYAFII LFILDLIRLN WPFFNMLYCR TVGALMRKKE IHTYNGVLWY
     151  ILGLIFSFNF FSKDVTLISL FLLSWSDTAA ATIGRKYGHL TPKVARNKSL
     201  AGSIAAFTVG VITCWVFYGY FVPAYSYVNK PGEIQWSPET SRLSLNMLSL
     251  LGGVVAALSE GIDLFNWDDN FTIPVLSSLF MNAVIKTFKK *

external links for Dgk1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Dgk1p

1)	Han GS, et al. (2008) Characterization of the Yeast DGK1-encoded CTP-dependent Diacylglycerol Kinase. J Biol Chem 283(29):20443-53
2)	Han GS, et al. (2008) An unconventional diacylglycerol kinase that regulates phospholipid synthesis and nuclear membrane growth. J Biol Chem 283(29):20433-42
3)	Kosodo Y, et al. (2001) Multicopy suppressors of the sly1 temperature-sensitive mutation in the ER-Golgi vesicular transport in Saccharomyces cerevisiae. Yeast 18(11):1003-14
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41