TPO4/YOR273C Protein Information Help

Standard Name Tpo4p 1
Systematic Name Yor273cp
ORF Classification Verified
Description Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane (1, 2, 3)
Name Description Transporter of POlyamines 4
Experimental Data
Molecules/cell 21400 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 659
Molecular Weight (Da) 73,222
Isoelectric Point (pI) 8.33

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tpo4p (InterPro)
Transmembrane Domains There are 11 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 25 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000006BE89 | Q12256
MIPS: YOR273C
NCBI: 1279700 | 1420612 | 6324847 | 74627269 | NP_014916.1
GenBank/EMBL/DDBJ: DAA11039.1 | X89633 | Z75181
External Classifications TC: 2.A.1.2 [The Drug:H+ Antiporter-1 (12 Spanner) (DHA1) Family]
Amino Acid Sequence (or in FASTA format)
 
       1  MPSSLTKTES NSDPRTNIQQ VPKALDKNVT NSGNLDSTSS STGSITEDEK
      51  RSEPNADSNN MTGGEPIDPR DLDWDGPDDP DNPHNWSSLK KWYTTMTSAF
     101  LCLVVTMGSS LYVSSVPELV ERYHVSQTLA LAGLTFYLLG LSTVIGAPLS
     151  EVFGRKPVYL FSLPVSMLFT MGVGLSNGHM RIILPLRFLS GVFASPALSV
     201  GSGTILDIFD VDQVSVAMTY FVLSPFLGPV LSPIMAGFAT EAKGWRWSEW
     251  IQLIAGGLIL PFIALMPETH KGIILRKRAK KRNIALKKFS REAQKEFLKT
     301  TVTITILRPL KMLVVEPIVF VFSVYVAFIF AILFGFFEAY AVIYRGVYHM
     351  SMGISGLPFI GIGVGLWIGA FFYLYIDRKY LFPKPPAGTQ PLTEKERTSK
     401  RTTPYRGARD AETGELLPVV PEKFLIACKF GSVALPIGLF WQAWTARSDV
     451  HWMAPVAAGV PFGFGLILIF FSVLMYFSTC YPPLTVASCL AANNLLRYVM
     501  SSVFPLFTIQ MYTKMKIKWA STLFALVCVV MIPIPWVFEK WGSKLRHKSQ
     551  FGYAAMEKEA ETEGGIDDVN AVDGELNLTR MTTLRTMETD PSTREKPGER
     601  LSLRRTHTQP VPASFDREDG QHAQNRNEPI SNSLYSAIKD NEDGYSYTEM
     651  ATDASARMV*

external links for Tpo4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome TCDB
YOGY DIP YeastRC Structure Prediction (Seattle)

GeneMANIA

References cited on this page View Complete Literature Guide for Tpo4p
1) Tomitori H, et al.  (2001) Multiple polyamine transport systems on the vacuolar membrane in yeast. Biochem J 353(Pt 3):681-8
2) Albertsen M, et al.  (2003) Localization and function of the yeast multidrug transporter Tpo1p. J Biol Chem 278(15):12820-5
3) Dias PJ, et al.  (2010) Evolution of the 12-Spanner Drug:H+ Antiporter DHA1 Family in Hemiascomycetous Yeasts. OMICS 14(6):701-10
4) Tomitori H, et al.  (1999) Identification of a gene for a polyamine transport protein in yeast. J Biol Chem 274(6):3265-7
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41