PAC1/YOR269W Protein Information

Standard Name	Pac1p 1
Systematic Name	Yor269wp
ORF Classification	Verified
Description	Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly (2, 3, 4)
Name Description	Perish in the Absence of Cin8p 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	494
Molecular Weight (Da)	56,900
Isoelectric Point (pI)	7.57

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pac1p (InterPro)
Physical Interactions	There are 78 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001311C8 \| P39946 MIPS: YOR269W NCBI: 1279696 \| 1330321 \| 1420604 \| 1709554 \| 6324843 \| NP_014912.1 GenBank/EMBL/DDBJ: DAA11035.1 \| U16827 \| X89633 \| Z75177

Amino Acid Sequence (or in FASTA format)
1 MTNWQQQLPL TDTQKNELDK SVLRYLNWNY KQTVRHEHAQ DYESVRHAIV 51 TLSGFLLQES VDRQEFISNN DTSNESMVDI DELLLPKKWN SIVRLQKKII 101 ELEQNTETLV SQIKDLNTQV SELAQFKPTT SNGTSAHNVL KWIPRNLPSC 151 LINVESSVTS VKLHPNLPIV FVATDHGKLY AFDLFNYTIP LASLQSHTKA 201 ITSMDVLFTN YTNSSKKNYL VIVTASKDLQ IHVFKWVSEE CKFQQIRSLL 251 GHEHIVSAVK IWQKNNDVHI ASCSRDQTVK IWDFHNGWSL KTFQPHSQWV 301 RSIDVLGDYI ISGSHDTTLR LTHWPSGNGL SVGTGHEFPI EKVKFIHFIE 351 DSPEIRFRTP STDRYKNWGM QYCVSASRDR TIKIWEIPLP TLMAHRAPIP 401 NPTDSNFRCV LTLKGHLSWV RDISIRGQYL FSCADDKSVR CWDLNTGQCL 451 HVWEKLHTGF VNCLDLDVDF DSNVTPRQMM VTGGLDCKSN VFMR*

Amino Acid Sequence (or in FASTA format)

       1  MTNWQQQLPL TDTQKNELDK SVLRYLNWNY KQTVRHEHAQ DYESVRHAIV
      51  TLSGFLLQES VDRQEFISNN DTSNESMVDI DELLLPKKWN SIVRLQKKII
     101  ELEQNTETLV SQIKDLNTQV SELAQFKPTT SNGTSAHNVL KWIPRNLPSC
     151  LINVESSVTS VKLHPNLPIV FVATDHGKLY AFDLFNYTIP LASLQSHTKA
     201  ITSMDVLFTN YTNSSKKNYL VIVTASKDLQ IHVFKWVSEE CKFQQIRSLL
     251  GHEHIVSAVK IWQKNNDVHI ASCSRDQTVK IWDFHNGWSL KTFQPHSQWV
     301  RSIDVLGDYI ISGSHDTTLR LTHWPSGNGL SVGTGHEFPI EKVKFIHFIE
     351  DSPEIRFRTP STDRYKNWGM QYCVSASRDR TIKIWEIPLP TLMAHRAPIP
     401  NPTDSNFRCV LTLKGHLSWV RDISIRGQYL FSCADDKSVR CWDLNTGQCL
     451  HVWEKLHTGF VNCLDLDVDF DSNVTPRQMM VTGGLDCKSN VFMR*

external links for Pac1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Pac1p

1)	Geiser JR, et al. (1997) Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Mol Biol Cell 8(6):1035-50
2)	Fujiwara T, et al. (1999) Bni1p regulates microtubule-dependent nuclear migration through the actin cytoskeleton in Saccharomyces cerevisiae. Mol Cell Biol 19(12):8016-27
3)	Lee WL, et al. (2003) The role of the lissencephaly protein Pac1 during nuclear migration in budding yeast. J Cell Biol 160(3):355-64
4)	Huang J, et al. (2012) Lis1 Acts as a "Clutch" between the ATPase and Microtubule-Binding Domains of the Dynein Motor. Cell 150(5):975-86