PNT1/YOR266W Protein Information Help

Standard Name Pnt1p 1
Systematic Name Yor266wp
ORF Classification Verified
Description Mitochondrial integral inner membrane protein; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine (1, 2, 3)
Name Description PeNTamidine resistance 1
Experimental Data
Molecules/cell 1460 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 423
Molecular Weight (Da) 48,934
Isoelectric Point (pI) 10.29

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pnt1p (InterPro)
Transmembrane Domains There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 7 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000131D60 | P38969
MIPS: YOR266W
NCBI: 1420598 | 2507101 | 398366001 | 51012995 | 557056 | 940373 | NP_014909.3 | NM_001183685.3
GenBank/EMBL/DDBJ: AAA86313.1 | AAB08526.1 | AAT92791.1 | CAA99489.1 | DAA11032.1 | AY692772 | U15217 | U30184 | Z75174
Amino Acid Sequence (or in FASTA format)
       1  MDSRVALVRK YIAPSVIKSD SIQLHGLVKA PLFKALNSRY KLGSLQIVQD
      51  VDWNAKTTPS DSPEPLAATL NSNRSLPMTK FPKQEILEQV KLDTKVGKWR
     101  KFMTGWFRIG LYLLKSYKTG IQNTLKVFWD TRNEEQKFSI KNGALANLVR
     151  EIEMHEINTR LSSSSLPTSS SAKAPLRPLS INRKTLVELI RRDQIWKLPV
     201  FFTLVFIFEE VSVLIFTFFP RVCPYNCLTP GGYKKLSNSY IKGTTSTQGN
     251  YGLGPLEFTK QGTIKYEPPY AVPIENLYNF LTSFPQSMIS NWKLYIYKKL
     301  KLQKLLCNEI EKIYQYLFID DWLLLQSILN TDVEKTKIAL SDRELVNCIL
     351  ERKLYHMGDD LNEMVNDTLG KEILLKRLFL YWTLRYNDTI SLNGKHTFSE
     401  KWGVNNISLL KYNSELVATK DIQ*                            

external links for Pnt1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) Organelle DB
Candida (CGD) CYC2008 (complexes) MIPS YPL+
YGOB Complexome Pfam domains YeastGFP
YOGY DIP
YeastRC Public Image Repository

GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Pnt1p
1) Ludewig G and Staben C  (1994) Characterization of the PNT1 pentamidine resistance gene of Saccharomyces cerevisiae. Antimicrob Agents Chemother 38(12):2850-6
2) He S and Fox TD  (1999) Mutations affecting a yeast mitochondrial inner membrane protein, pnt1p, block export of a mitochondrially synthesized fusion protein from the matrix. Mol Cell Biol 19(10):6598-607
3) Saracco SA and Fox TD  (2002) Cox18p is required for export of the mitochondrially encoded Saccharomyces cerevisiae Cox2p C-tail and interacts with Pnt1p and Mss2p in the inner membrane. Mol Biol Cell 13(4):1122-31
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41