RPT4/YOR259C Protein Information

Standard Name	Rpt4p 1, 2
Systematic Name	Yor259cp
Alias	Crl13p , Pcs1p , Sug2p
ORF Classification	Verified
Description	One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization (3, 4, 5, 6, 7)
Name Description	Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	437
Molecular Weight (Da)	49,408
Isoelectric Point (pI)	5.54

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rpt4p (InterPro)
Physical Interactions	There are 159 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000531AA \| P53549 MIPS: YOR259C NCBI: 1147800 \| 1420585 \| 1931623 \| 2506090 \| 398365977 \| 403071972 \| NP_014902.3 \| NM_001183678.3 GenBank/EMBL/DDBJ: DAA11026.1 \| U43720 \| U93262 \| Z75167

Amino Acid Sequence (or in FASTA format)
1 MSEEQDPLLA GLGETSGDNH TQQSHEQQPE QPQETEEHHE EEPSRVDPEQ 51 EAHNKALNQF KRKLLEHRRY DDQLKQRRQN IRDLEKLYDK TENDIKALQS 101 IGQLIGEVMK ELSEEKYIVK ASSGPRYIVG VRNSVDRSKL KKGVRVTLDI 151 TTLTIMRILP RETDPLVYNM TSFEQGEITF DGIGGLTEQI RELREVIELP 201 LKNPEIFQRV GIKPPKGVLL YGPPGTGKTL LAKAVAATIG ANFIFSPASG 251 IVDKYIGESA RIIREMFAYA KEHEPCIIFM DEVDAIGGRR FSEGTSADRE 301 IQRTLMELLT QMDGFDNLGQ TKIIMATNRP DTLDPALLRP GRLDRKVEIP 351 LPNEAGRLEI FKIHTAKVKK TGEFDFEAAV KMSDGFNGAD IRNCATEAGF 401 FAIRDDRDHI NPDDLMKAVR KVAEVKKLEG TIEYQKL*

Amino Acid Sequence (or in FASTA format)

       1  MSEEQDPLLA GLGETSGDNH TQQSHEQQPE QPQETEEHHE EEPSRVDPEQ
      51  EAHNKALNQF KRKLLEHRRY DDQLKQRRQN IRDLEKLYDK TENDIKALQS
     101  IGQLIGEVMK ELSEEKYIVK ASSGPRYIVG VRNSVDRSKL KKGVRVTLDI
     151  TTLTIMRILP RETDPLVYNM TSFEQGEITF DGIGGLTEQI RELREVIELP
     201  LKNPEIFQRV GIKPPKGVLL YGPPGTGKTL LAKAVAATIG ANFIFSPASG
     251  IVDKYIGESA RIIREMFAYA KEHEPCIIFM DEVDAIGGRR FSEGTSADRE
     301  IQRTLMELLT QMDGFDNLGQ TKIIMATNRP DTLDPALLRP GRLDRKVEIP
     351  LPNEAGRLEI FKIHTAKVKK TGEFDFEAAV KMSDGFNGAD IRNCATEAGF
     401  FAIRDDRDHI NPDDLMKAVR KVAEVKKLEG TIEYQKL*

external links for Rpt4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rpt4p

1)	Finley D, et al. (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2)	McCusker, J.H. and J.E. Haber (1996) SUG2 {crl13} is identical to CRL13. Personal Communication
3)	Glickman MH, et al. (1998) The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol 18(6):3149-62
4)	McDonald HB and Byers B (1997) A proteasome cap subunit required for spindle pole body duplication in yeast. J Cell Biol 137(3):539-53
5)	Russell SJ, et al. (1999) Subcellular localization, stoichiometry, and protein levels of 26 S proteasome subunits in yeast. J Biol Chem 274(31):21943-52
6)	Rubin DM, et al. (1998) Active site mutants in the six regulatory particle ATPases reveal multiple roles for ATP in the proteasome. EMBO J 17(17):4909-19
7)	Lipson C, et al. (2008) A proteasomal ATPase contributes to dislocation of endoplasmic reticulum-associated degradation (ERAD) substrates. J Biol Chem 283(11):7166-75