APC5/YOR249C Protein Information Help

Standard Name Apc5p 1
Systematic Name Yor249cp
Alias Rmc1p 2
ORF Classification Verified
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress (1, 3, 4, 5)
Name Description Anaphase Promoting Complex 1
Experimental Data
Molecules/cell 259 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 685
Molecular Weight (Da) 79,274
Isoelectric Point (pI) 4.94

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Apc5p (InterPro)
Physical Interactions There are 71 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000006B3EA | Q08683
MIPS: YOR249C
NCBI: 1420565 | 398365935 | 55976292 | NP_014892.3
GenBank/EMBL/DDBJ: DAA11016.1 | Z75157
Amino Acid Sequence (or in FASTA format)
 
       1  MSKYGPLGIT NFITPYDLCI LILIHAHCSQ DNGISVPTAV FLRLISPTRP
      51  SLEWNPLLKD NSNLRSSSIV PPPVLPILDN IIRILLDDKD GNKIALTLMG
     101  YLEAINGLDS INRLMMDLEK NCLVNNYRSM KMRTTSTRRQ MTRASFLGTF
     151  LSTCIRKYQI GDFEMRETIW INLQNFKTVF KHTPLWLRFK DNVHIQKVKN
     201  CLLANDEISV EDQQMVEFFQ HFNNGNDADS KTMNEENYGT LISIQHLQSI
     251  VNRQIVNWLD NTEFNLMGQE ETSSTYEEQS GLVFDLLDTL SLNDATKFPL
     301  IFILKYLEAI KENSYQTALD SLHNYFDYKS TGNSQNYFHI SLLSLATFHS
     351  SFNECDAAIN SFEEATRIAR ENKDMETLNL IMIWIINFIE VHPEYANRFY
     401  ITVEQIIKYL KNSSDVEDAN IFSNAYKFET LLSMVKESKT AEVSSSLLKF
     451  MAITLQNVPS QNFDLFQSLV SYEVKFWKEL GYESISDVYE KFLSKTSSSS
     501  LRNYDSSIIN QDIKVAFKAL EEDDFLKVKQ YLLKSESLEL DYDQKINLKY
     551  LRVKYLVKIG DYDLSMRLIN QYVKECCEEV ADSNWRFKFE IESINVLLLS
     601  DVGIRSLPKI IKLIDEYKEI GNPLRCVILL LKLCEVLIQV GKSMEAECLI
     651  SCNLSTILEF PFVRKKTDEL LESLSVEEDR DVQMT*

external links for Apc5p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Apc5p
1) Zachariae W, et al.  (1998) Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science 279(5354):1216-9
2) Harkness TA, et al.  (2002) The ubiquitin-dependent targeting pathway in Saccharomyces cerevisiae plays a critical role in multiple chromatin assembly regulatory steps. Genetics 162(2):615-32
3) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
4) Buschhorn BA, et al.  (2011) Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1. Nat Struct Mol Biol 18(1):6-13
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41