ENV9/YOR246C Protein Information

Standard Name	Env9p 1
Systematic Name	Yor246cp
ORF Classification	Verified
Description	Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts (1, 2, 3, 4)
Name Description	late ENdosome and Vacuole interface function 1

Experimental Data
Molecules/cell	967 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	330
Molecular Weight (Da)	37,484
Isoelectric Point (pI)	8.35

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Env9p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 6 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000069E66 \| Q08651 MIPS: YOR246C NCBI: 1420560 \| 398365915 \| 74655023 \| NP_014889.3 GenBank/EMBL/DDBJ: DAA11014.1 \| Z75154
External Classifications	EC: 1.1.1.- [Oxidoreductases With NAD+ or NADP+ as acceptor]

Amino Acid Sequence (or in FASTA format)
1 MLDPRILPYY DPAVERKIAV VTGGNTGIGW YTVLHLYLHG FVVYICGRNS 51 HKISKAIQEI LAEAKKRCHE DDDGSSPGAG PGPSIQRLGS LHYIHLDLTD 101 LKCVERAALK ILKLEDHIDV LVNNAGIMAV PLEMTKDGFE VQLQTNYISH 151 FIFTMRLLPL LRHCRGRIIS LSSIGHHLEF MYWKLSKTWD YKPNMLFTWF 201 RYAMSKTALI QCTKMLAIKY PDVLCLSVHP GLVMNTNLFS YWTRLPIVGI 251 FFWLLFQVVG FFFGVSNEQG SLASLKCALD PNLSVEKDNG KYFTTGGKES 301 KSSYVSNNVD EAASTWIWTV HQLRDRGFDI *

Amino Acid Sequence (or in FASTA format)

       1  MLDPRILPYY DPAVERKIAV VTGGNTGIGW YTVLHLYLHG FVVYICGRNS
      51  HKISKAIQEI LAEAKKRCHE DDDGSSPGAG PGPSIQRLGS LHYIHLDLTD
     101  LKCVERAALK ILKLEDHIDV LVNNAGIMAV PLEMTKDGFE VQLQTNYISH
     151  FIFTMRLLPL LRHCRGRIIS LSSIGHHLEF MYWKLSKTWD YKPNMLFTWF
     201  RYAMSKTALI QCTKMLAIKY PDVLCLSVHP GLVMNTNLFS YWTRLPIVGI
     251  FFWLLFQVVG FFFGVSNEQG SLASLKCALD PNLSVEKDNG KYFTTGGKES
     301  KSSYVSNNVD EAASTWIWTV HQLRDRGFDI *

external links for Env9p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Env9p

1)	Ricarte F, et al. (2011) A Genome-Wide Immunodetection Screen in S. cerevisiae Uncovers Novel Genes Involved in Lysosomal Vacuole Function and Morphology. PLoS One 6(8):e23696
2)	Boyer J, et al. (1996) Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae. Yeast 12(15):1575-86
3)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4)	Kushner DB, et al. (2003) Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus. Proc Natl Acad Sci U S A 100(26):15764-9
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41