PUS7/YOR243C Protein Information Help

Standard Name Pus7p 1
Systematic Name Yor243cp
ORF Classification Verified
Description Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria (1, 2, 3, 4)
Name Description PseudoUridine Synthase 1
Experimental Data
Molecules/cell 4150 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 676
Molecular Weight (Da) 77,002
Isoelectric Point (pI) 7.9

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pus7p (InterPro)
Physical Interactions There are 16 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052EF0 | Q08647
MIPS: YOR243C
NCBI: 1420554 | 2501603 | 6324817 | NP_014886.1
GenBank/EMBL/DDBJ: DAA11011.1 | Z75151
External Classifications EC: 5.4.99.- [Intramolecular Transferases, Transferring Other Groups]
EC: 5.4.99.27 [tRNA pseudouridine(13) synthase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSDSSEATVK RPLDAHVGPS ENAAKKLKIE QRTQADGIHE ADVGITLFLS
      51  PELPGFRGQI KQRYTDFLVN EIDQEGKVIH LTDKGFKMPK KPQRSKEEVN
     101  AEKESEAARR QEFNVDPELR NQLVEIFGEE DVLKIESVYR TANKMETAKN
     151  FEDKSVRTKI HQLLREAFKN ELESVTTDTN TFKIARSNRN SRTNKQEKIN
     201  QTRDANGVEN WGYGPSKDFI HFTLHKENKD TMEAVNVITK LLRVPSRVIR
     251  YAGTKDRRAV TCQRVSISKI GLDRLNALNR TLKGMIIGNY NFSDASLNLG
     301  DLKGNEFVVV IRDVTTGNSE VSLEEIVSNG CKSLSENGFI NYFGMQRFGT
     351  FSISTHTIGR ELLLSNWKKA AELILSDQDN VLPKSKEARK IWAETKDAAL
     401  ALKQMPRQCL AENALLYSLS NQRKEEDGTY SENAYYTAIM KIPRNLRTMY
     451  VHAYQSYVWN SIASKRIELH GLKLVVGDLV IDTSEKSPLI SGIDDEDFDE
     501  DVREAQFIRA KAVTQEDIDS VKYTMEDVVL PSPGFDVLYP SNEELKQLYV
     551  DILKADNMDP FNMRRKVRDF SLAGSYRTVI QKPKSLEYRI IHYDDPSQQL
     601  VNTDLDILNN TRAKESGQKY MKAKLDRYMP DKGGEKTAVV LKFQLGTSAY
     651  ATMALRELMK LETSRRGDMC DVKENI*

external links for Pus7p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Pus7p
1) Ma X, et al.  (2003) Pseudouridylation (Psi) of U2 snRNA in S. cerevisiae is catalyzed by an RNA-independent mechanism. EMBO J 22(8):1889-97
2) Behm-Ansmant I, et al.  (2003) The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs. RNA 9(11):1371-82
3) Decatur WA and Schnare MN  (2008) Different mechanisms for Pseudouridine formation in yeast 5S and 5.8S rRNAs. Mol Cell Biol 28(10):3089-100
4) Wu G, et al.  (2011) U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP. EMBO J 30(1):79-89
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41