ABP140/YOR239W Protein Information Help

Standard Name Abp140p 1
Systematic Name Yor239wp
Alias Yor240wp , Trm140p 2, 3
ORF Classification Verified
Description AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift (1, 2, 3)
Name Description Actin Binding Protein
Experimental Data
Molecules/cell 17000 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 628
Molecular Weight (Da) 71,485
Isoelectric Point (pI) 4.44

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Abp140p (InterPro)
Physical Interactions There are 19 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000016841C | Q08641
MIPS: YOR239W
NCBI: 1420547 | 1420548 | 18202486 | 398365899 | NP_014882.4 | NM_001183658.4
GenBank/EMBL/DDBJ: CAA99460.1 | CAA99461.1 | DAA11008.1 | Z75147
External Classifications EC: 2.1.1.- [Transferases transferring one-carbon groups methyltransferases]
EC: 2.1.1.268 [tRNA(Thr) (cytosine(32)-N(3))-methyltransferase]
Amino Acid Sequence (or in FASTA format)
       1  MGVADLIKKF ESISKEEGDA TVDTNSSSKP LKSNDETKEL HQQESTAVPQ
      51  EVDVNEEFEN EPETINSSRT AEKPLETNLP KPETNEEDEE EGSMSENKIY
     101  SKGENADINV NDFQEYKEME NTGAEVLASS VEESDAIQEG VAEETEGIAT
     151  PKQKENEKND ESEEESANNA SEPAEEYSQS EEDADIEQSN GKETENAENA
     201  SQQANDGSTS TTTSKNKKKK NKKKNKKKRN GNVNTNANVD DSTKTGENDD
     251  TTGDTTSSTT SAIQEVNDLE VVDDSCLGID QQHNREHLKA LTQDVKEETL
     301  ENIAHEGRGD NTGDQNAVEK SDFEKSDTEG SRIGRDLPFE FGKRNLTEES
     351  DVWDHNAWDN VEWGEEQVQQ AEEKIKEQFK HPVPEFDKKL YNENPARYWD
     401  IFYKNNKENF FKDRKWLQIE FPILYASTRK DAEPVTIFEI GCGAGNTFFP
     451  ILKDNENENL RIIAADFAPR AVELVKNSEQ FNPKYGHATV WDLANPDGNL
     501  PDGVEPHSVD IAVMIFVFSA LAPNQWDQAM DNLHKILKPG GKIIFRDYGA
     551  YDLTQVRFKK NRILEENFYV RGDGTRVYFF SEEKLREIFT KKYFLENKIG
     601  TDRRLLVNRK RQLKMYRCWV QAVFDVPQ*                       

external links for Abp140p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) Organelle DB
Candida (CGD) CYC2008 (complexes) MIPS YPL+
YGOB Complexome Pfam domains YeastGFP
YOGY DIP YeastRC Structure Prediction (Seattle) YeastRC Public Image Repository

GeneMANIA


IMP


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Abp140p
1) Asakura T, et al.  (1998) Isolation and characterization of a novel actin filament-binding protein from Saccharomyces cerevisiae. Oncogene 16(1):121-30
2) Noma A, et al.  (2011) Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae. RNA 17(6):1111-9
3) D'Silva S, et al.  (2011) A domain of the actin binding protein Abp140 is the yeast methyltransferase responsible for 3-methylcytidine modification in the tRNA anti-codon loop. RNA 17(6):1100-10
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41