ABP140/YOR239W Protein Information

Standard Name	Abp140p 1
Systematic Name	Yor239wp
Alias	Yor240wp , Trm140p 2, 3
ORF Classification	Verified
Description	AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift (1, 2, 3)
Name Description	Actin Binding Protein

Experimental Data
Molecules/cell	17000 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	628
Molecular Weight (Da)	71,485
Isoelectric Point (pI)	4.44

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Abp140p (InterPro)
Physical Interactions	There are 17 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000016841C \| Q08641 MIPS: YOR239W NCBI: 1420547 \| 1420548 \| 18202486 \| 398365899 \| NP_014882.4 GenBank/EMBL/DDBJ: DAA11008.1 \| Z75147
External Classifications	EC: 2.1.1.- [Transferases transferring one-carbon groups methyltransferases]

Amino Acid Sequence (or in FASTA format)
1 MGVADLIKKF ESISKEEGDA TVDTNSSSKP LKSNDETKEL HQQESTAVPQ 51 EVDVNEEFEN EPETINSSRT AEKPLETNLP KPETNEEDEE EGSMSENKIY 101 SKGENADINV NDFQEYKEME NTGAEVLASS VEESDAIQEG VAEETEGIAT 151 PKQKENEKND ESEEESANNA SEPAEEYSQS EEDADIEQSN GKETENAENA 201 SQQANDGSTS TTTSKNKKKK NKKKNKKKRN GNVNTNANVD DSTKTGENDD 251 TTGDTTSSTT SAIQEVNDLE VVDDSCLGID QQHNREHLKA LTQDVKEETL 301 ENIAHEGRGD NTGDQNAVEK SDFEKSDTEG SRIGRDLPFE FGKRNLTEES 351 DVWDHNAWDN VEWGEEQVQQ AEEKIKEQFK HPVPEFDKKL YNENPARYWD 401 IFYKNNKENF FKDRKWLQIE FPILYASTRK DAEPVTIFEI GCGAGNTFFP 451 ILKDNENENL RIIAADFAPR AVELVKNSEQ FNPKYGHATV WDLANPDGNL 501 PDGVEPHSVD IAVMIFVFSA LAPNQWDQAM DNLHKILKPG GKIIFRDYGA 551 YDLTQVRFKK NRILEENFYV RGDGTRVYFF SEEKLREIFT KKYFLENKIG 601 TDRRLLVNRK RQLKMYRCWV QAVFDVPQ*

Amino Acid Sequence (or in FASTA format)

       1  MGVADLIKKF ESISKEEGDA TVDTNSSSKP LKSNDETKEL HQQESTAVPQ
      51  EVDVNEEFEN EPETINSSRT AEKPLETNLP KPETNEEDEE EGSMSENKIY
     101  SKGENADINV NDFQEYKEME NTGAEVLASS VEESDAIQEG VAEETEGIAT
     151  PKQKENEKND ESEEESANNA SEPAEEYSQS EEDADIEQSN GKETENAENA
     201  SQQANDGSTS TTTSKNKKKK NKKKNKKKRN GNVNTNANVD DSTKTGENDD
     251  TTGDTTSSTT SAIQEVNDLE VVDDSCLGID QQHNREHLKA LTQDVKEETL
     301  ENIAHEGRGD NTGDQNAVEK SDFEKSDTEG SRIGRDLPFE FGKRNLTEES
     351  DVWDHNAWDN VEWGEEQVQQ AEEKIKEQFK HPVPEFDKKL YNENPARYWD
     401  IFYKNNKENF FKDRKWLQIE FPILYASTRK DAEPVTIFEI GCGAGNTFFP
     451  ILKDNENENL RIIAADFAPR AVELVKNSEQ FNPKYGHATV WDLANPDGNL
     501  PDGVEPHSVD IAVMIFVFSA LAPNQWDQAM DNLHKILKPG GKIIFRDYGA
     551  YDLTQVRFKK NRILEENFYV RGDGTRVYFF SEEKLREIFT KKYFLENKIG
     601  TDRRLLVNRK RQLKMYRCWV QAVFDVPQ*

external links for Abp140p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Abp140p

1)	Asakura T, et al. (1998) Isolation and characterization of a novel actin filament-binding protein from Saccharomyces cerevisiae. Oncogene 16(1):121-30
2)	Noma A, et al. (2011) Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae. RNA 17(6):1111-9
3)	D'Silva S, et al. (2011) A domain of the actin binding protein Abp140 is the yeast methyltransferase responsible for 3-methylcytidine modification in the tRNA anti-codon loop. RNA 17(6):1100-10
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41