STE4/YOR212W Protein Information

Standard Name	Ste4p 1
Systematic Name	Yor212wp
Alias	Hmd2p 2
ORF Classification	Verified
Description	G protein beta subunit, forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats (3, 4)
Name Description	STErile 1

Experimental Data
Molecules/cell	2050 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	423
Molecular Weight (Da)	46,581
Isoelectric Point (pI)	6.11

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ste4p (InterPro)
Physical Interactions	There are 91 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012B225 \| P18851 MIPS: YOR212W NCBI: 1050764 \| 1346108 \| 1420495 \| 172755 \| 398365783 \| NP_014855.3 \| NM_001183631.3 GenBank/EMBL/DDBJ: DAA10984.1 \| M23982 \| X92441 \| Z75120

Amino Acid Sequence (or in FASTA format)
1 MAAHQMDSIT YSNNVTQQYI QPQSLQDISA VEDEIQNKIE AARQESKQLH 51 AQINKAKHKI QDASLFQMAN KVTSLTKNKI NLKPNIVLKG HNNKISDFRW 101 SRDSKRILSA SQDGFMLIWD SASGLKQNAI PLDSQWVLSC AISPSSTLVA 151 SAGLNNNCTI YRVSKENRVA QNVASIFKGH TCYISDIEFT DNAHILTASG 201 DMTCALWDIP KAKRVREYSD HLGDVLALAI PEEPNSENSS NTFASCGSDG 251 YTYIWDSRSP SAVQSFYVND SDINALRFFK DGMSIVAGSD NGAINMYDLR 301 SDCSIATFSL FRGYEERTPT PTYMAANMEY NTAQSPQTLK STSSSYLDNQ 351 GVVSLDFSAS GRLMYSCYTD IGCVVWDVLK GEIVGKLEGH GGRVTGVRSS 401 PDGLAVCTGS WDSTMKIWSP GYQ*

Amino Acid Sequence (or in FASTA format)

       1  MAAHQMDSIT YSNNVTQQYI QPQSLQDISA VEDEIQNKIE AARQESKQLH
      51  AQINKAKHKI QDASLFQMAN KVTSLTKNKI NLKPNIVLKG HNNKISDFRW
     101  SRDSKRILSA SQDGFMLIWD SASGLKQNAI PLDSQWVLSC AISPSSTLVA
     151  SAGLNNNCTI YRVSKENRVA QNVASIFKGH TCYISDIEFT DNAHILTASG
     201  DMTCALWDIP KAKRVREYSD HLGDVLALAI PEEPNSENSS NTFASCGSDG
     251  YTYIWDSRSP SAVQSFYVND SDINALRFFK DGMSIVAGSD NGAINMYDLR
     301  SDCSIATFSL FRGYEERTPT PTYMAANMEY NTAQSPQTLK STSSSYLDNQ
     351  GVVSLDFSAS GRLMYSCYTD IGCVVWDVLK GEIVGKLEGH GGRVTGVRSS
     401  PDGLAVCTGS WDSTMKIWSP GYQ*

external links for Ste4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Ste4p

1)	Hartwell LH (1980) Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone. J Cell Biol 85(3):811-22
2)	Sugimoto K, et al. (1995) Dosage suppressors of the dominant G1 cyclin mutant CLN3-2: identification of a yeast gene encoding a putative RNA/ssDNA binding protein. Mol Gen Genet 248(6):712-8
3)	Whiteway M, et al. (1989) The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein. Cell 56(3):467-77
4)	Bar EE, et al. (2003) Gbetagamma recruits Rho1 to the site of polarized growth during mating in budding yeast. J Biol Chem 278(24):21798-804
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41