MCA1/YOR197W Protein Information

Standard Name	Mca1p 1
Systematic Name	Yor197wp
Alias	Yca1p 2
ORF Classification	Verified
Description	Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization (2, 3, 4, 5) Also known as: [MCA] 6
Name Description	MetaCAspase 1

Experimental Data
Molecules/cell	1400 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	432
Molecular Weight (Da)	47,982
Isoelectric Point (pI)	5.09

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mca1p (InterPro)
Physical Interactions	There are 34 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000D796A5 \| Q08601 MIPS: YOR197W NCBI: 1420469 \| 189047114 \| 394986400 \| 398365705 \| 51013115 \| NP_014840.4 GenBank/EMBL/DDBJ: DAA10972.1 \| AY692832 \| Z75105
External Classifications	EC: 3.4.22.- [Cysteine endopeptidases]

Amino Acid Sequence (or in FASTA format)
1 MYPGSGRYTY NNAGGNNGYQ RPMAPPPNQQ YGQQYGQQYE QQYGQQYGQQ 51 NDQQFSQQYA PPPGPPPMAY NRPVYPPPQF QQEQAKAQLS NGYNNPNVNA 101 SNMYGPPQNM SLPPPQTQTI QGTDQPYQYS QCTGRRKALI IGINYIGSKN 151 QLRGCINDAH NIFNFLTNGY GYSSDDIVIL TDDQNDLVRV PTRANMIRAM 201 QWLVKDAQPN DSLFLHYSGH GGQTEDLDGD EEDGMDDVIY PVDFETQGPI 251 IDDEMHDIMV KPLQQGVRLT ALFDSCHSGT VLDLPYTYST KGIIKEPNIW 301 KDVGQDGLQA AISYATGNRA ALIGSLGSIF KTVKGGMGNN VDRERVRQIK 351 FSAADVVMLS GSKDNQTSAD AVEDGQNTGA MSHAFIKVMT LQPQQSYLSL 401 LQNMRKELAG KYSQKPQLSS SHPIDVNLQF IM*

Amino Acid Sequence (or in FASTA format)

       1  MYPGSGRYTY NNAGGNNGYQ RPMAPPPNQQ YGQQYGQQYE QQYGQQYGQQ
      51  NDQQFSQQYA PPPGPPPMAY NRPVYPPPQF QQEQAKAQLS NGYNNPNVNA
     101  SNMYGPPQNM SLPPPQTQTI QGTDQPYQYS QCTGRRKALI IGINYIGSKN
     151  QLRGCINDAH NIFNFLTNGY GYSSDDIVIL TDDQNDLVRV PTRANMIRAM
     201  QWLVKDAQPN DSLFLHYSGH GGQTEDLDGD EEDGMDDVIY PVDFETQGPI
     251  IDDEMHDIMV KPLQQGVRLT ALFDSCHSGT VLDLPYTYST KGIIKEPNIW
     301  KDVGQDGLQA AISYATGNRA ALIGSLGSIF KTVKGGMGNN VDRERVRQIK
     351  FSAADVVMLS GSKDNQTSAD AVEDGQNTGA MSHAFIKVMT LQPQQSYLSL
     401  LQNMRKELAG KYSQKPQLSS SHPIDVNLQF IM*

external links for Mca1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Mca1p

1)	Szallies A, et al. (2002) A metacaspase of Trypanosoma brucei causes loss of respiration competence and clonal death in the yeast Saccharomyces cerevisiae. FEBS Lett 517(1-3):144-50
2)	Madeo F, et al. (2002) A caspase-related protease regulates apoptosis in yeast. Mol Cell 9(4):911-7
3)	Lee RE, et al. (2008) A non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics. PLoS ONE 3(8):e2956
4)	Lee RE, et al. (2010) Metacaspase Yca1 is required for clearance of insoluble protein aggregates. Proc Natl Acad Sci U S A 107(30):13348-53
5)	Wong AH, et al. (2012) Crystal structure of the yeast metacaspase Yca1. J Biol Chem 287(35):29251-9
6)	Nemecek J, et al. (2009) A prion of yeast metacaspase homolog (Mca1p) detected by a genetic screen. Proc Natl Acad Sci U S A 106(6):1892-6
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41