GSP2/YOR185C Protein Information

Standard Name	Gsp2p
Systematic Name	Yor185cp
Alias	Cnr2p 1
ORF Classification	Verified
Description	GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); not required for viability; protein abundance increases in response to DNA replication stress; GSP2 has a paralog, GSP1, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description	Genetic Suppressor of Prp20-1 2

Experimental Data
Molecules/cell	2460 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	220
Molecular Weight (Da)	24,990
Isoelectric Point (pI)	6.67

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gsp2p (InterPro)
Physical Interactions	There are 34 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012BC17 \| P32836 MIPS: YOR185C NCBI: 1420445 \| 171623 \| 311754 \| 417091 \| 51013685 \| 6324759 \| NP_014828.1 \| NM_001183604.1 GenBank/EMBL/DDBJ: DAA10957.1 \| AY693117 \| L08691 \| X71946 \| Z75093

Amino Acid Sequence (or in FASTA format)
1 MSAPAQNNAE VPTFKLVLVG DGGTGKTTFV KRHLTGEFEK KYIATIGVEV 51 HPLSFYTNFG EIKFDVWDTA GQEKFGGLRD GYYINAQCAI IMFDVTSRIT 101 YKNVPNWHRD LVRVCENIPI VLCGNKVDVK ERKVKAKTIT FHRKKNLQYY 151 DISAKSNYNF EKPFLWLARK LAGNPQLEFV ASPALAPPEV QVDEQLMHQY 201 QQEMDQATAL PLPDEDDADL *

Amino Acid Sequence (or in FASTA format)

       1  MSAPAQNNAE VPTFKLVLVG DGGTGKTTFV KRHLTGEFEK KYIATIGVEV
      51  HPLSFYTNFG EIKFDVWDTA GQEKFGGLRD GYYINAQCAI IMFDVTSRIT
     101  YKNVPNWHRD LVRVCENIPI VLCGNKVDVK ERKVKAKTIT FHRKKNLQYY
     151  DISAKSNYNF EKPFLWLARK LAGNPQLEFV ASPALAPPEV QVDEQLMHQY
     201  QQEMDQATAL PLPDEDDADL *

external links for Gsp2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Gsp2p

1)	Kadowaki T, et al. (1993) Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily. EMBO J 12(7):2929-37
2)	Belhumeur P, et al. (1993) GSP1 and GSP2, genetic suppressors of the prp20-1 mutant in Saccharomyces cerevisiae: GTP-binding proteins involved in the maintenance of nuclear organization. Mol Cell Biol 13(4):2152-61
3)	Lau D, et al. (2000) Purification of protein A-tagged yeast ran reveals association with a novel karyopherin beta family member, Pdr6p. J Biol Chem 275(1):467-71
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41