MED4/YOR174W Protein Information

Standard Name	Med4p 1
Systematic Name	Yor174wp
ORF Classification	Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 2)
Name Description	MEDiator complex 1

Experimental Data
Molecules/cell	1620 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	284
Molecular Weight (Da)	32,205
Isoelectric Point (pI)	4.36

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Med4p (InterPro)
Physical Interactions	There are 151 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005313D \| Q12343 MIPS: YOR174W NCBI: 1293733 \| 1420423 \| 398365565 \| 74645046 \| NP_014817.3 GenBank/EMBL/DDBJ: DAA10946.1 \| U55021 \| Z75082

Amino Acid Sequence (or in FASTA format)
1 MSVQDTKAVE FSMGHIRSSS VSLVAEATSN TNSEDKLSKV QLYEDLCRYE 51 DTLSKLVESV DRFKPNLDIA KDLIRTDEAL FENVKLLAEY DNIYRNLQKI 101 DKDSEELDSK TRKILEILNE CHDELKALPM LEQVEFEKNT ILQQRSKINS 151 TELLDYATKL SKFTKIPPTF DKGAVGPNNF IWPAEDALRR GMLAMASLHS 201 KELTRIPGEE VEETEVPTVP PSQSEEQKGQ MAKKEGTPKT DSFIFDGTAK 251 EVGDEADNTK DKEKEENNDD ALDLDLDLFD PDDF*

Amino Acid Sequence (or in FASTA format)

       1  MSVQDTKAVE FSMGHIRSSS VSLVAEATSN TNSEDKLSKV QLYEDLCRYE
      51  DTLSKLVESV DRFKPNLDIA KDLIRTDEAL FENVKLLAEY DNIYRNLQKI
     101  DKDSEELDSK TRKILEILNE CHDELKALPM LEQVEFEKNT ILQQRSKINS
     151  TELLDYATKL SKFTKIPPTF DKGAVGPNNF IWPAEDALRR GMLAMASLHS
     201  KELTRIPGEE VEETEVPTVP PSQSEEQKGQ MAKKEGTPKT DSFIFDGTAK
     251  EVGDEADNTK DKEKEENNDD ALDLDLDLFD PDDF*

external links for Med4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Med4p

1)	Myers LC, et al. (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
2)	Kornberg RD (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41