GLN4/YOR168W Protein Information Help

Standard Name Gln4p
Systematic Name Yor168wp
ORF Classification Verified
Description Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions (1, 2, 3)
Name Description GLutamiNe metabolism
Gene Product glutamyl-tRNA synthetase 1
Experimental Data
Molecules/cell 37500 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 809
Molecular Weight (Da) 93,132
Isoelectric Point (pI) 8.7

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Gln4p (InterPro)
Physical Interactions There are 15 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013662B | P13188
MIPS: YOR168W
NCBI: 1293729 | 1420413 | 171604 | 2507436 | 358439875 | 398365535 | NP_014811.3
GenBank/EMBL/DDBJ: DAA10942.1 | M29184 | M29185 | U55021 | Z75076
External Classifications EC: 6.1.1.18 [Glutamine--tRNA ligase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSSVEELTQL FSQVGFEDKK VKEIVKNKKV SDSLYKLIKE TPSDYQWNKS
      51  TRALVHNLAS FVKGTDLPKS ELIVNGIING DLKTSLQVDA AFKYVKANGE
     101  ASTKMGMNEN SGVGIEITED QVRNYVMQYI QENKERILTE RYKLVPGIFA
     151  DVKNLKELKW ADPRSFKPII DQEVLKLLGP KDERDLIKKK TKNNEKKKTN
     201  SAKKSSDNSA SSGPKRTMFN EGFLGDLHKV GENPQAYPEL MKEHLEVTGG
     251  KVRTRFPPEP NGYLHIGHSK AIMVNFGYAK YHNGTCYLRF DDTNPEKEAP
     301  EYFESIKRMV SWLGFKPWKI TYSSDYFDEL YRLAEVLIKN GKAYVCHCTA
     351  EEIKRGRGIK EDGTPGGERY ACKHRDQSIE QNLQEFRDMR DGKYKPGEAI
     401  LRMKQDLNSP SPQMWDLIAY RVLNAPHPRT GTKWRIYPTY DFTHCLVDSM
     451  ENITHSLCTT EFYLSRESYE WLCDQVHVFR PAQREYGRLN ITGTVLSKRK
     501  IAQLVDEKFV RGWDDPRLFT LEAIRRRGVP PGAILSFINT LGVTTSTTNI
     551  QVVRFESAVR KYLEDTTPRL MFVLDPVEVV VDNLSDDYEE LATIPYRPGT
     601  PEFGERTVPF TNKFYIERSD FSENVDDKEF FRLTPNQPVG LIKVSHTVSF
     651  KSLEKDEAGK IIRIHVNYDN KVEEGSKPKK PKTYIQWVPI SSKYNSPLRV
     701  TETRVYNQLF KSENPSSHPE GFLKDINPES EVVYKESVME HNFGDVVKNS
     751  PWVVDSVKNS EFYVEEDKDS KEVCRFQAMR VGYFTLDKES TTSKVILNRI
     801  VSLKDATSK*

external links for Gln4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Gln4p
1) Ludmerer SW and Schimmel P  (1985) Cloning of GLN4: an essential gene that encodes glutaminyl-tRNA synthetase in Saccharomyces cerevisiae. J Bacteriol 163(2):763-8
2) Ludmerer SW, et al.  (1993) Purification of glutamine tRNA synthetase from Saccharomyces cerevisiae. A monomeric aminoacyl-tRNA synthetase with a large and dispensable NH2-terminal domain. J Biol Chem 268(8):5519-23
3) Whelihan EF and Schimmel P  (1997) Rescuing an essential enzyme-RNA complex with a non-essential appended domain. EMBO J 16(10):2968-74
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41