PDR5/YOR153W Protein Information

Standard Name	Pdr5p 1
Systematic Name	Yor153wp
Alias	Lem1p , Ydr1p , Sts1p
ORF Classification	Verified
Description	Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth; PDR5 has a paralog, PDR15, that arose from the whole genome duplication (10, 11, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description	Pleiotropic Drug Resistance 1

Experimental Data
Molecules/cell	42000 12

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,511
Molecular Weight (Da)	170,437
Isoelectric Point (pI)	7.75

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pdr5p (InterPro)
Transmembrane Domains	There are 14 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 56 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004C4D5 \| P33302 MIPS: YOR153W NCBI: 1293713 \| 1321667 \| 1420383 \| 395259 \| 398365429 \| 402501 \| 464819 \| NP_014796.3 GenBank/EMBL/DDBJ: DAA10926.1 \| D26548 \| L19922 \| U55020 \| X74113 \| Z75061
External Classifications	TC: 3.A.1.205 [The Pleiotropic Drug Resistance (PDR) Family]

Amino Acid Sequence (or in FASTA format)
1 MPEAKLNNNV NDVTSYSSAS SSTENAADLH NYNGFDEHTE ARIQKLARTL 51 TAQSMQNSTQ SAPNKSDAQS IFSSGVEGVN PIFSDPEAPG YDPKLDPNSE 101 NFSSAAWVKN MAHLSAADPD FYKPYSLGCA WKNLSASGAS ADVAYQSTVV 151 NIPYKILKSG LRKFQRSKET NTFQILKPMD GCLNPGELLV VLGRPGSGCT 201 TLLKSISSNT HGFDLGADTK ISYSGYSGDD IKKHFRGEVV YNAEADVHLP 251 HLTVFETLVT VARLKTPQNR IKGVDRESYA NHLAEVAMAT YGLSHTRNTK 301 VGNDIVRGVS GGERKRVSIA EVSICGSKFQ CWDNATRGLD SATALEFIRA 351 LKTQADISNT SATVAIYQCS QDAYDLFNKV CVLDDGYQIY YGPADKAKKY 401 FEDMGYVCPS RQTTADFLTS VTSPSERTLN KDMLKKGIHI PQTPKEMNDY 451 WVKSPNYKEL MKEVDQRLLN DDEASREAIK EAHIAKQSKR ARPSSPYTVS 501 YMMQVKYLLI RNMWRLRNNI GFTLFMILGN CSMALILGSM FFKIMKKGDT 551 STFYFRGSAM FFAILFNAFS SLLEIFSLYE ARPITEKHRT YSLYHPSADA 601 FASVLSEIPS KLIIAVCFNI IFYFLVDFRR NGGVFFFYLL INIVAVFSMS 651 HLFRCVGSLT KTLSEAMVPA SMLLLALSMY TGFAIPKKKI LRWSKWIWYI 701 NPLAYLFESL LINEFHGIKF PCAEYVPRGP AYANISSTES VCTVVGAVPG 751 QDYVLGDDFI RGTYQYYHKD KWRGFGIGMA YVVFFFFVYL FLCEYNEGAK 801 QKGEILVFPR SIVKRMKKRG VLTEKNANDP ENVGERSDLS SDRKMLQESS 851 EEESDTYGEI GLSKSEAIFH WRNLCYEVQI KAETRRILNN VDGWVKPGTL 901 TALMGASGAG KTTLLDCLAE RVTMGVITGD ILVNGIPRDK SFPRSIGYCQ 951 QQDLHLKTAT VRESLRFSAY LRQPAEVSIE EKNRYVEEVI KILEMEKYAD 1001 AVVGVAGEGL NVEQRKRLTI GVELTAKPKL LVFLDEPTSG LDSQTAWSIC 1051 QLMKKLANHG QAILCTIHQP SAILMQEFDR LLFMQRGGKT VYFGDLGEGC 1101 KTMIDYFESH GAHKCPADAN PAEWMLEVVG AAPGSHANQD YYEVWRNSEE 1151 YRAVQSELDW MERELPKKGS ITAAEDKHEF SQSIIYQTKL VSIRLFQQYW 1201 RSPDYLWSKF ILTIFNQLFI GFTFFKAGTS LQGLQNQMLA VFMFTVIFNP 1251 ILQQYLPSFV QQRDLYEARE RPSRTFSWIS FIFAQIFVEV PWNILAGTIA 1301 YFIYYYPIGF YSNASAAGQL HERGALFWLF SCAFYVYVGS MGLLVISFNQ 1351 VAESAANLAS LLFTMSLSFC GVMTTPSAMP RFWIFMYRVS PLTYFIQALL 1401 AVGVANVDVK CADYELLEFT PPSGMTCGQY MEPYLQLAKT GYLTDENATD 1451 TCSFCQISTT NDYLANVNSF YSERWRNYGI FICYIAFNYI AGVFFYWLAR 1501 VPKKNGKLSK K*

Amino Acid Sequence (or in FASTA format)

       1  MPEAKLNNNV NDVTSYSSAS SSTENAADLH NYNGFDEHTE ARIQKLARTL
      51  TAQSMQNSTQ SAPNKSDAQS IFSSGVEGVN PIFSDPEAPG YDPKLDPNSE
     101  NFSSAAWVKN MAHLSAADPD FYKPYSLGCA WKNLSASGAS ADVAYQSTVV
     151  NIPYKILKSG LRKFQRSKET NTFQILKPMD GCLNPGELLV VLGRPGSGCT
     201  TLLKSISSNT HGFDLGADTK ISYSGYSGDD IKKHFRGEVV YNAEADVHLP
     251  HLTVFETLVT VARLKTPQNR IKGVDRESYA NHLAEVAMAT YGLSHTRNTK
     301  VGNDIVRGVS GGERKRVSIA EVSICGSKFQ CWDNATRGLD SATALEFIRA
     351  LKTQADISNT SATVAIYQCS QDAYDLFNKV CVLDDGYQIY YGPADKAKKY
     401  FEDMGYVCPS RQTTADFLTS VTSPSERTLN KDMLKKGIHI PQTPKEMNDY
     451  WVKSPNYKEL MKEVDQRLLN DDEASREAIK EAHIAKQSKR ARPSSPYTVS
     501  YMMQVKYLLI RNMWRLRNNI GFTLFMILGN CSMALILGSM FFKIMKKGDT
     551  STFYFRGSAM FFAILFNAFS SLLEIFSLYE ARPITEKHRT YSLYHPSADA
     601  FASVLSEIPS KLIIAVCFNI IFYFLVDFRR NGGVFFFYLL INIVAVFSMS
     651  HLFRCVGSLT KTLSEAMVPA SMLLLALSMY TGFAIPKKKI LRWSKWIWYI
     701  NPLAYLFESL LINEFHGIKF PCAEYVPRGP AYANISSTES VCTVVGAVPG
     751  QDYVLGDDFI RGTYQYYHKD KWRGFGIGMA YVVFFFFVYL FLCEYNEGAK
     801  QKGEILVFPR SIVKRMKKRG VLTEKNANDP ENVGERSDLS SDRKMLQESS
     851  EEESDTYGEI GLSKSEAIFH WRNLCYEVQI KAETRRILNN VDGWVKPGTL
     901  TALMGASGAG KTTLLDCLAE RVTMGVITGD ILVNGIPRDK SFPRSIGYCQ
     951  QQDLHLKTAT VRESLRFSAY LRQPAEVSIE EKNRYVEEVI KILEMEKYAD
    1001  AVVGVAGEGL NVEQRKRLTI GVELTAKPKL LVFLDEPTSG LDSQTAWSIC
    1051  QLMKKLANHG QAILCTIHQP SAILMQEFDR LLFMQRGGKT VYFGDLGEGC
    1101  KTMIDYFESH GAHKCPADAN PAEWMLEVVG AAPGSHANQD YYEVWRNSEE
    1151  YRAVQSELDW MERELPKKGS ITAAEDKHEF SQSIIYQTKL VSIRLFQQYW
    1201  RSPDYLWSKF ILTIFNQLFI GFTFFKAGTS LQGLQNQMLA VFMFTVIFNP
    1251  ILQQYLPSFV QQRDLYEARE RPSRTFSWIS FIFAQIFVEV PWNILAGTIA
    1301  YFIYYYPIGF YSNASAAGQL HERGALFWLF SCAFYVYVGS MGLLVISFNQ
    1351  VAESAANLAS LLFTMSLSFC GVMTTPSAMP RFWIFMYRVS PLTYFIQALL
    1401  AVGVANVDVK CADYELLEFT PPSGMTCGQY MEPYLQLAKT GYLTDENATD
    1451  TCSFCQISTT NDYLANVNSF YSERWRNYGI FICYIAFNYI AGVFFYWLAR
    1501  VPKKNGKLSK K*

external links for Pdr5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	TCDB
YGOB	DIP	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Pdr5p

1)	Leppert G, et al. (1990) Cloning by gene amplification of two loci conferring multiple drug resistance in Saccharomyces. Genetics 125(1):13-20
2)	Mahe Y, et al. (1996) The ATP binding cassette transporters Pdr5 and Snq2 of Saccharomyces cerevisiae can mediate transport of steroids in vivo. J Biol Chem 271(41):25167-72
3)	Balzi E, et al. (1994) PDR5, a novel yeast multidrug resistance conferring transporter controlled by the transcription regulator PDR1. J Biol Chem 269(3):2206-14
4)	Miyahara K, et al. (1996) The involvement of the Saccharomyces cerevisiae multidrug resistance transporters Pdr5p and Snq2p in cation resistance. FEBS Lett 399(3):317-20
5)	Bissinger PH and Kuchler K (1994) Molecular cloning and expression of the Saccharomyces cerevisiae STS1 gene product. A yeast ABC transporter conferring mycotoxin resistance. J Biol Chem 269(6):4180-6
6)	Egner R, et al. (1995) Endocytosis and vacuolar degradation of the plasma membrane-localized Pdr5 ATP-binding cassette multidrug transporter in Saccharomyces cerevisiae. Mol Cell Biol 15(11):5879-87
7)	Egner R and Kuchler K (1996) The yeast multidrug transporter Pdr5 of the plasma membrane is ubiquitinated prior to endocytosis and degradation in the vacuole. FEBS Lett 378(2):177-81
8)	Rogers B, et al. (2001) The pleitropic drug ABC transporters from Saccharomyces cerevisiae. J Mol Microbiol Biotechnol 3(2):207-14
9)	Emter R, et al. (2002) ERG6 and PDR5 regulate small lipophilic drug accumulation in yeast cells via distinct mechanisms. FEBS Lett 521(1-3):57-61
10)	Mamnun YM, et al. (2004) Expression regulation of the yeast PDR5 ATP-binding cassette (ABC) transporter suggests a role in cellular detoxification during the exponential growth phase. FEBS Lett 559(1-3):111-7
11)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
12)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41