RPB2/YOR151C Protein Information Help

Standard Name Rpb2p 1, 2
Systematic Name Yor151cp
Alias Rpb150p 3 , Rpo22p 4, 5 , Sit2p 6 , Soh2p 7, 8
ORF Classification Verified
Description RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit (10, 11, 12, 13, 9)
Name Description RNA Polymerase B 2
Gene Product B150 14
Experimental Data
Molecules/cell 18700 15
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,224
Molecular Weight (Da) 138,750
Isoelectric Point (pI) 6.5

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rpb2p (InterPro)
Physical Interactions There are 240 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052E85 | P08518
MIPS: YOR151C
NCBI: 110590855 | 122920062 | 122920077 | 122920092 | 122921031 | 122921067 | 122921083 | 122921098 | 122921110 | 1293711 | 134104675 | 134104690 | 134104711 | 134104723 | 134104735 | 1420379 | 14278325 | 14278336 | 14278366 | 146387185 | 160877774 | 161172228 | 161172241 | 172211 | 190613463 | 194368735 | 20150714 | 224983591 | 239781917 | 239781930 | 239781943 | 239781956 | 239781969 | 239781983 | 239781996 | 239782009 | 240104512 | 2507349 | 254839442 | 254839457 | 254839472 | 254839487 | 254839502 | 254839517 | 254839532 | 257471947 | 257471962 | 266618766 | 269914553 | 295982476 | 295982489 | 30749617 | 30749738 | 31581374 | 325053964 | 325053979 | 326634539 | 343197457 | 343197469 | 343197481 | 343197493 | 343197505 | 343197517 | 343197529 | 343197541 | 343197553 | 343197565 | 343197577 | 343781198 | 34810554 | 357380837 | 359546291 | 359546309 | 359546326 | 359546343 | 359546360 | 359546377 | 359546393 | 359546410 | 374977880 | 374977895 | 380764980 | 398365417 | 414145447 | 414145460 | 459358295 | 46015416 | 46015781 | 56965912 | 56965924 | 56966108 | 56966122 | 56966132 | 56966142 | 56966152 | 58177165 | 61679445 | 61679462 | 61679503 | 61679516 | 83754244 | NP_014794.3
GenBank/EMBL/DDBJ: DAA10924.1 | AF527884 | M15693 | U55020 | Z75059
External Classifications EC: 2.7.7.6 [DNA-directed RNA polymerase]
Amino Acid Sequence (or in FASTA format)
       1  MSDLANSEKY YDEDPYGFED ESAPITAEDS WAVISAFFRE KGLVSQQLDS
      51  FNQFVDYTLQ DIICEDSTLI LEQLAQHTTE SDNISRKYEI SFGKIYVTKP
     101  MVNESDGVTH ALYPQEARLR NLTYSSGLFV DVKKRTYEAI DVPGRELKYE
     151  LIAEESEDDS ESGKVFIGRL PIMLRSKNCY LSEATESDLY KLKECPFDMG
     201  GYFIINGSEK VLIAQERSAG NIVQVFKKAA PSPISHVAEI RSALEKGSRF
     251  ISTLQVKLYG REGSSARTIK ATLPYIKQDI PIVIIFRALG IIPDGEILEH
     301  ICYDVNDWQM LEMLKPCVED GFVIQDRETA LDFIGRRGTA LGIKKEKRIQ
     351  YAKDILQKEF LPHITQLEGF ESRKAFFLGY MINRLLLCAL DRKDQDDRDH
     401  FGKKRLDLAG PLLAQLFKTL FKKLTKDIFR YMQRTVEEAH DFNMKLAINA
     451  KTITSGLKYA LATGNWGEQK KAMSSRAGVS QVLNRYTYSS TLSHLRRTNT
     501  PIGRDGKLAK PRQLHNTHWG LVCPAETPEG QACGLVKNLS LMSCISVGTD
     551  PMPIITFLSE WGMEPLEDYV PHQSPDATRV FVNGVWHGVH RNPARLMETL
     601  RTLRRKGDIN PEVSMIRDIR EKELKIFTDA GRVYRPLFIV EDDESLGHKE
     651  LKVRKGHIAK LMATEYQDIE GGFEDVEEYT WSSLLNEGLV EYIDAEEEES
     701  ILIAMQPEDL EPAEANEEND LDVDPAKRIR VSHHATTFTH CEIHPSMILG
     751  VAASIIPFPD HNQSPRNTYQ SAMGKQAMGV FLTNYNVRMD TMANILYYPQ
     801  KPLGTTRAME YLKFRELPAG QNAIVAIACY SGYNQEDSMI MNQSSIDRGL
     851  FRSLFFRSYM DQEKKYGMSI TETFEKPQRT NTLRMKHGTY DKLDDDGLIA
     901  PGVRVSGEDV IIGKTTPISP DEEELGQRTA YHSKRDASTP LRSTENGIVD
     951  QVLVTTNQDG LKFVKVRVRT TKIPQIGDKF ASRHGQKGTI GITYRREDMP
    1001  FTAEGIVPDL IINPHAIPSR MTVAHLIECL LSKVAALSGN EGDASPFTDI
    1051  TVEGISKLLR EHGYQSRGFE VMYNGHTGKK LMAQIFFGPT YYQRLRHMVD
    1101  DKIHARARGP MQVLTRQPVE GRSRDGGLRF GEMERDCMIA HGAASFLKER
    1151  LMEASDAFRV HICGICGLMT VIAKLNHNQF ECKGCDNKID IYQIHIPYAA
    1201  KLLFQELMAM NITPRLYTDR SRDF*                           

external links for Rpb2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Mass Spec (Seattle)


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rpb2p
1) Sweetser D, et al.  (1987) Prokaryotic and eukaryotic RNA polymerases have homologous core subunits. Proc Natl Acad Sci U S A 84(5):1192-6
2) Nonet M, et al.  (1987) Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis. Mol Cell Biol 7(5):1602-11
3) Riva M, et al.  (1986) Isolation of structural genes for yeast RNA polymerases by immunological screening. Proc Natl Acad Sci U S A 83(6):1554-8
4) Jansma DB, et al.  (1996) Similar upstream regulatory elements of genes that encode the two largest subunits of RNA polymerase II in Saccharomyces cerevisiae. Nucleic Acids Res 24(22):4543-51
5) Ingles CJ, et al.  (1984) Identification, molecular cloning, and mutagenesis of Saccharomyces cerevisiae RNA polymerase genes. Proc Natl Acad Sci U S A 81(7):2157-61
6) Arndt KT, et al.  (1989) A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatases. Cell 56(4):527-37
7) Fan HY and Klein HL  (1994) Characterization of mutations that suppress the temperature-sensitive growth of the hpr1 delta mutant of Saccharomyces cerevisiae. Genetics 137(4):945-56
8) Fan HY, et al.  (1996) Mutations in the RNA polymerase II transcription machinery suppress the hyperrecombination mutant hpr1 delta of Saccharomyces cerevisiae. Genetics 142(3):749-59
9) Archambault J and Friesen JD  (1993) Genetics of eukaryotic RNA polymerases I, II, and III. Microbiol Rev 57(3):703-24
10) Hampsey M  (1998) Molecular genetics of the RNA polymerase II general transcriptional machinery. Microbiol Mol Biol Rev 62(2):465-503
11) Cramer P, et al.  (2000) Architecture of RNA polymerase II and implications for the transcription mechanism. Science 288(5466):640-9
12) Geiduschek EP and Kassavetis GA  (2001) The RNA polymerase III transcription apparatus. J Mol Biol 310(1):1-26
13) Cramer P  (2002) Multisubunit RNA polymerases. Curr Opin Struct Biol 12(1):89-97
14) Sentenac A  (1985) Eukaryotic RNA polymerases. CRC Crit Rev Biochem 18(1):31-90
15) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41