EFT1/YOR133W Protein Information

Standard Name	Eft1p 1
Systematic Name	Yor133wp
ORF Classification	Verified
Description	Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	Elongation Factor Two 3

Experimental Data
Molecules/cell	82700 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	842
Molecular Weight (Da)	93,288
Isoelectric Point (pI)	6.25

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Eft1p (InterPro)
Physical Interactions	There are 78 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000534E7 \| P32324 MIPS: YOR133W NCBI: 1050821 \| 1164977 \| 119389349 \| 119390550 \| 119390551 \| 1420342 \| 149242996 \| 149242998 \| 149243000 \| 149243001 \| 171442 \| 190613579 \| 190613581 \| 190613583 \| 190613585 \| 190613587 \| 190613589 \| 190613591 \| 190613593 \| 190613595 \| 192988336 \| 192988338 \| 192988340 \| 195927600 \| 27065817 \| 27065818 \| 28948705 \| 416935 \| 47176791 \| 49258821 \| 549849 \| 55670150 \| 6320593 \| 6324707 \| 67463994 \| 67463996 \| 67463998 \| 67464000 \| 67464002 \| 67464004 \| 67464008 \| 67464010 \| 67464012 \| 67464014 \| 67464016 \| 67464018 \| 849206 \| 927318 \| NP_010673.1 \| NP_014776.1 GenBank/EMBL/DDBJ: DAA10907.1 \| DAA12229.1 \| AY497635 \| M59369 \| M59370 \| U28373 \| U32274 \| X90518 \| X94335 \| Z75041

Amino Acid Sequence (or in FASTA format)
1 MVAFTVDQMR SLMDKVTNVR NMSVIAHVDH GKSTLTDSLV QRAGIISAAK 51 AGEARFTDTR KDEQERGITI KSTAISLYSE MSDEDVKEIK QKTDGNSFLI 101 NLIDSPGHVD FSSEVTAALR VTDGALVVVD TIEGVCVQTE TVLRQALGER 151 IKPVVVINKV DRALLELQVS KEDLYQTFAR TVESVNVIVS TYADEVLGDV 201 QVYPARGTVA FGSGLHGWAF TIRQFATRYA KKFGVDKAKM MDRLWGDSFF 251 NPKTKKWTNK DTDAEGKPLE RAFNMFILDP IFRLFTAIMN FKKDEIPVLL 301 EKLEIVLKGD EKDLEGKALL KVVMRKFLPA ADALLEMIVL HLPSPVTAQA 351 YRAEQLYEGP ADDANCIAIK NCDPKADLML YVSKMVPTSD KGRFYAFGRV 401 FAGTVKSGQK VRIQGPNYVP GKKDDLFIKA IQRVVLMMGR FVEPIDDCPA 451 GNIIGLVGID QFLLKTGTLT TSETAHNMKV MKFSVSPVVQ VAVEVKNAND 501 LPKLVEGLKR LSKSDPCVLT YMSESGEHIV AGTGELHLEI CLQDLEHDHA 551 GVPLKISPPV VAYRETVESE SSQTALSKSP NKHNRIYLKA EPIDEEVSLA 601 IENGIINPRD DFKARARIMA DDYGWDVTDA RKIWCFGPDG NGPNLVIDQT 651 KAVQYLHEIK DSVVAAFQWA TKEGPIFGEE MRSVRVNILD VTLHADAIHR 701 GGGQIIPTMR RATYAGFLLA DPKIQEPVFL VEIQCPEQAV GGIYSVLNKK 751 RGQVVSEEQR PGTPLFTVKA YLPVNESFGF TGELRQATGG QAFPQMVFDH 801 WSTLGSDPLD PTSKAGEIVL AARKRHGMKE EVPGWQEYYD KL*

Amino Acid Sequence (or in FASTA format)

       1  MVAFTVDQMR SLMDKVTNVR NMSVIAHVDH GKSTLTDSLV QRAGIISAAK
      51  AGEARFTDTR KDEQERGITI KSTAISLYSE MSDEDVKEIK QKTDGNSFLI
     101  NLIDSPGHVD FSSEVTAALR VTDGALVVVD TIEGVCVQTE TVLRQALGER
     151  IKPVVVINKV DRALLELQVS KEDLYQTFAR TVESVNVIVS TYADEVLGDV
     201  QVYPARGTVA FGSGLHGWAF TIRQFATRYA KKFGVDKAKM MDRLWGDSFF
     251  NPKTKKWTNK DTDAEGKPLE RAFNMFILDP IFRLFTAIMN FKKDEIPVLL
     301  EKLEIVLKGD EKDLEGKALL KVVMRKFLPA ADALLEMIVL HLPSPVTAQA
     351  YRAEQLYEGP ADDANCIAIK NCDPKADLML YVSKMVPTSD KGRFYAFGRV
     401  FAGTVKSGQK VRIQGPNYVP GKKDDLFIKA IQRVVLMMGR FVEPIDDCPA
     451  GNIIGLVGID QFLLKTGTLT TSETAHNMKV MKFSVSPVVQ VAVEVKNAND
     501  LPKLVEGLKR LSKSDPCVLT YMSESGEHIV AGTGELHLEI CLQDLEHDHA
     551  GVPLKISPPV VAYRETVESE SSQTALSKSP NKHNRIYLKA EPIDEEVSLA
     601  IENGIINPRD DFKARARIMA DDYGWDVTDA RKIWCFGPDG NGPNLVIDQT
     651  KAVQYLHEIK DSVVAAFQWA TKEGPIFGEE MRSVRVNILD VTLHADAIHR
     701  GGGQIIPTMR RATYAGFLLA DPKIQEPVFL VEIQCPEQAV GGIYSVLNKK
     751  RGQVVSEEQR PGTPLFTVKA YLPVNESFGF TGELRQATGG QAFPQMVFDH
     801  WSTLGSDPLD PTSKAGEIVL AARKRHGMKE EVPGWQEYYD KL*

external links for Eft1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Eft1p

1)	Perentesis JP, et al. (1992) Saccharomyces cerevisiae elongation factor 2. Genetic cloning, characterization of expression, and G-domain modeling. J Biol Chem 267(2):1190-7
2)	Justice MC, et al. (1998) Elongation factor 2 as a novel target for selective inhibition of fungal protein synthesis. J Biol Chem 273(6):3148-51
3)	Dunlop PC and Bodley JW (1983) Biosynthetic labeling of diphthamide in Saccharomyces cerevisiae. J Biol Chem 258(8):4754-8
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41