RGA1/YOR127W Protein Information

Standard Name	Rga1p
Systematic Name	Yor127wp
Alias	Dbm1p 1 , The1p
ORF Classification	Verified
Description	GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; RGA1 has a paralog, RGA2, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description	Rho GTPase Activating Protein 2

Experimental Data
Molecules/cell	396 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,007
Molecular Weight (Da)	112,830
Isoelectric Point (pI)	6.36

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rga1p (InterPro)
Physical Interactions	There are 40 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000133814 \| P39083 MIPS: YOR127W NCBI: 1050813 \| 1164971 \| 1420330 \| 1835220 \| 460973 \| 553148 \| 6324701 \| 729298 \| NP_014770.1 GenBank/EMBL/DDBJ: DAA10901.1 \| L02617 \| U07421 \| X90518 \| X90950 \| X94335 \| Z75035

Amino Acid Sequence (or in FASTA format)
1 MASTAPNEQF PSCVRCKEFI TTGHAYELGC DRWHTHCFAC YKCEKPLSCE 51 SDFLVLGTGA LICFDCSDSC KNCGKKIDDL AIILSSSNEA YCSDCFKCCK 101 CGENIADLRY AKTKRGLFCL SCHEKLLAKR KYYEEKKRRL KKNLPSLPTP 151 VIDNGHTDEV SASAVLPEKT FSRPASLVNE IPSGSEPSKD IETNSSDIVP 201 HFITGYNDSD DNSGSSKFGS NVSIDVIGPE ENSTEHVNDD VKEEAEAPSA 251 NMSLNVATDP TLSCKEPPSH SRNLLNKTPL RNSSGQYLAK SPSSYRQGII 301 VNDSLEESDQ IDPPNNSSRN ASELLTSVLH SPVSVNMKNP KGSNTDIFNT 351 GEISQMDPSL SRKVLNNIVE ETNALQRPVV EVVKEDRSVP DLAGVQQEQA 401 EKYSYSNNSG KGRKISRSLS RRSKDLMINL KSRATGKQDS NVKLSPASKV 451 TSRRSQDLMR DNDSHTGLDT PNSNSTSLDI LVNNQKSLNY KRFTDNGTLR 501 VTSGKETALE EQKNHSFKSP SPIDHLLQSP ATPSNVSMYR TPPLDSSLTF 551 DRRNGSSYSN QNYSIPSWQK TPKTQLENSD NFEEQKETLY ENSESRNDPS 601 LDKEIVTAEH YLKQLKINLK ELESQREELM KEITEMKSMK EALRRHIESY 651 NSEKNKLYLD SNELSNNPPM INEISLGESP PVKHVATASS VARSSVKPKF 701 WKFFSSAKPQ TEQSIQGVST NNTNSIVKSA PVLLSAPSSG SNSGRLEISP 751 PVLQNPNEFS DVRLVPIEND ANMGQSKDGE EYLDGSNLYG SSLVARCNYE 801 NNEIPMILSV CIDFIESDEE NMRSEGIYRK SGSQLVIEEI EKQFSAWKVQ 851 QNTETPNILT EQDLNVVTGV LKRYLRKLPN PIFTFQIYEP LMRLVKSKKM 901 MENLPFVGGK LSLEAKNSDT YMSSKSALKN ILEDLPREHY RVLRVLSEHI 951 EKVTRYSHWN RMTLYNLALV FAPGLIRDFS GEKDIIDMKE RNYIVAFIFG 1001 NYKDILT*

Amino Acid Sequence (or in FASTA format)

       1  MASTAPNEQF PSCVRCKEFI TTGHAYELGC DRWHTHCFAC YKCEKPLSCE
      51  SDFLVLGTGA LICFDCSDSC KNCGKKIDDL AIILSSSNEA YCSDCFKCCK
     101  CGENIADLRY AKTKRGLFCL SCHEKLLAKR KYYEEKKRRL KKNLPSLPTP
     151  VIDNGHTDEV SASAVLPEKT FSRPASLVNE IPSGSEPSKD IETNSSDIVP
     201  HFITGYNDSD DNSGSSKFGS NVSIDVIGPE ENSTEHVNDD VKEEAEAPSA
     251  NMSLNVATDP TLSCKEPPSH SRNLLNKTPL RNSSGQYLAK SPSSYRQGII
     301  VNDSLEESDQ IDPPNNSSRN ASELLTSVLH SPVSVNMKNP KGSNTDIFNT
     351  GEISQMDPSL SRKVLNNIVE ETNALQRPVV EVVKEDRSVP DLAGVQQEQA
     401  EKYSYSNNSG KGRKISRSLS RRSKDLMINL KSRATGKQDS NVKLSPASKV
     451  TSRRSQDLMR DNDSHTGLDT PNSNSTSLDI LVNNQKSLNY KRFTDNGTLR
     501  VTSGKETALE EQKNHSFKSP SPIDHLLQSP ATPSNVSMYR TPPLDSSLTF
     551  DRRNGSSYSN QNYSIPSWQK TPKTQLENSD NFEEQKETLY ENSESRNDPS
     601  LDKEIVTAEH YLKQLKINLK ELESQREELM KEITEMKSMK EALRRHIESY
     651  NSEKNKLYLD SNELSNNPPM INEISLGESP PVKHVATASS VARSSVKPKF
     701  WKFFSSAKPQ TEQSIQGVST NNTNSIVKSA PVLLSAPSSG SNSGRLEISP
     751  PVLQNPNEFS DVRLVPIEND ANMGQSKDGE EYLDGSNLYG SSLVARCNYE
     801  NNEIPMILSV CIDFIESDEE NMRSEGIYRK SGSQLVIEEI EKQFSAWKVQ
     851  QNTETPNILT EQDLNVVTGV LKRYLRKLPN PIFTFQIYEP LMRLVKSKKM
     901  MENLPFVGGK LSLEAKNSDT YMSSKSALKN ILEDLPREHY RVLRVLSEHI
     951  EKVTRYSHWN RMTLYNLALV FAPGLIRDFS GEKDIIDMKE RNYIVAFIFG
    1001  NYKDILT*

external links for Rga1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rga1p

1)	Chen GC, et al. (1996) The LIM domain-containing Dbm1 GTPase-activating protein is required for normal cellular morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol 16(4):1376-90
2)	Stevenson BJ, et al. (1995) Mutation of RGA1, which encodes a putative GTPase-activating protein for the polarity-establishment protein Cdc42p, activates the pheromone-response pathway in the yeast Saccharomyces cerevisiae. Genes Dev 9(23):2949-63
3)	Smith GR, et al. (2002) GTPase-activating proteins for Cdc42. Eukaryot Cell 1(3):469-80
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41