LEU9/YOR108W Protein Information

Standard Name	Leu9p 1
Systematic Name	Yor108wp
ORF Classification	Verified
Description	Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication (1, 2)
Name Description	LEUcine biosynthesis 1

Experimental Data
Molecules/cell	28800 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	604
Molecular Weight (Da)	67,200
Isoelectric Point (pI)	6.75

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Leu9p (InterPro)
Physical Interactions	There are 10 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006BF6A \| Q12166 MIPS: YOR108W NCBI: 1262141 \| 1420293 \| 6324682 \| 74645027 \| NP_014751.1 GenBank/EMBL/DDBJ: DAA10883.1 \| X94335 \| Z75016
External Classifications	EC: 2.3.3.13 [2-isopropylmalate synthase]

Amino Acid Sequence (or in FASTA format)
1 MVKHSFIALA EHASKLRRSI PPVKLTYKNM LRDPSVKYRA FAPPKMVKRI 51 WPDKTIQKAP RWLSTDLRDG NQSLPDPMSV AQKKEYFHKL INIGFKEIEV 101 SFPSASQTDF DFTRYAVENA PDDVGIQCLV QSREHLIKRT VEALTGAKRA 151 TIHTYLATSD MFREIVFNMS REEAISKAVE ATKLVRKLTK DDPSQQATRW 201 SYEFSPECFS DTPGEFAVEI CEAVKKAWEP TEENPIIFNL PATVEVASPN 251 VYADQIEYFS THITEREKVC ISTHCHNDRG CGVAATELGM LAGADRVEGC 301 LFGNGERTGN VDLVTVAMNM YTQGVSPNLD FSDLTSISEI VHRCNKIPIP 351 PRAPYGGELV VSAFSGSHQD AIKKGFAIQN KKQAQGETRW RIPYLPLDPK 401 DIGRDYEAVI RVNSQSGKGG AAWVIMRSLG LDVPRPMQVD FSNTLQKNAD 451 ALGRELKSEE ITKLFKETYN YNNNEHIYVT LLNYEVKKLN PERRALVGQV 501 EINDKVVNIE GYGNGPISSL VDALSNLLNV KLSVQNYSEH SLGSGSATQA 551 ASFINLSYIK DINNHATSNM WGVGVSEDTG DASIKAVFAT VNNIIHSGDV 601 LLAE*

Amino Acid Sequence (or in FASTA format)

       1  MVKHSFIALA EHASKLRRSI PPVKLTYKNM LRDPSVKYRA FAPPKMVKRI
      51  WPDKTIQKAP RWLSTDLRDG NQSLPDPMSV AQKKEYFHKL INIGFKEIEV
     101  SFPSASQTDF DFTRYAVENA PDDVGIQCLV QSREHLIKRT VEALTGAKRA
     151  TIHTYLATSD MFREIVFNMS REEAISKAVE ATKLVRKLTK DDPSQQATRW
     201  SYEFSPECFS DTPGEFAVEI CEAVKKAWEP TEENPIIFNL PATVEVASPN
     251  VYADQIEYFS THITEREKVC ISTHCHNDRG CGVAATELGM LAGADRVEGC
     301  LFGNGERTGN VDLVTVAMNM YTQGVSPNLD FSDLTSISEI VHRCNKIPIP
     351  PRAPYGGELV VSAFSGSHQD AIKKGFAIQN KKQAQGETRW RIPYLPLDPK
     401  DIGRDYEAVI RVNSQSGKGG AAWVIMRSLG LDVPRPMQVD FSNTLQKNAD
     451  ALGRELKSEE ITKLFKETYN YNNNEHIYVT LLNYEVKKLN PERRALVGQV
     501  EINDKVVNIE GYGNGPISSL VDALSNLLNV KLSVQNYSEH SLGSGSATQA
     551  ASFINLSYIK DINNHATSNM WGVGVSEDTG DASIKAVFAT VNNIIHSGDV
     601  LLAE*

external links for Leu9p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Leu9p

1)	Casalone E, et al. (2000) Identification by functional analysis of the gene encoding alpha-isopropylmalate synthase II (LEU9) in Saccharomyces cerevisiae. Yeast 16(6):539-45
2)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41