VPS21/YOR089C Protein Information 
| Standard Name | Vps21p 1, 2 |
|---|---|
| Systematic Name | Yor089cp |
| Alias | Vps12p 1, 2, 3 , Vpt12p 1 , Ypt21p , Ypt51p |
| ORF Classification | Verified |
| Description | Endosomal Rab family GTPase; required for endocytic transport and sorting of vacuolar hydrolases; required for endosomal localization of the CORVET complex; required with YPT52 for MVB biogenesis and sorting; involved in autophagy and ionic stress tolerance; geranylgeranylation required for membrane association; protein abundance increases in response to DNA replication stress; mammalian Rab5 homolog; VPS21 has a paralog, YPT53, that arose from the whole genome duplication (10, 4, 5, 6, 7, 8, 9) |
| Name Description | Vacuolar Protein Sorting 1, 2 |
| Experimental Data | |
|---|---|
| Molecules/cell | 8760 11 |
| Click on image for expanded interactive view |
|---|
| Post-translational Modifications | PhosphoGRID | PhosphoPep Database |
|---|---|
| Domains/motifs | See the graphical view and list of proteins that share domains/motifs in common with Vps21p (InterPro) |
| Signal Peptide(s) | There are 1 total predicted predicted signal peptide(s) (SignalP) |
| Physical Interactions | There are 82 total physical interactions (BioGRID) |
| Homologs | PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer |
| External Sequence Databases |
EBI: UPI000013BD32 | P36017 MIPS: YOR089C NCBI: 1164935 | 1420257 | 441477 | 483567 | 549795 | 6324663 | 7767071 | NP_014732.1 GenBank/EMBL/DDBJ: DAA10866.1 | X76173 | X94335 | Z29338 | Z74997 |
external links for Vps21p
| Homologs | Interaction Resources | Protein databases/Other | Localization Resources |
|---|---|---|---|
| BLASTP (NCBI) | BioGRID | SCOP Superfamily | Organelle DB |
| Ashbya (AGD) | BOND | GPMdb (Mass Spec.) | YPL+ |
| Aspergillus (AspGD) | BioPIXIE | MIPS | YeastGFP |
| Candida (CGD) | CYC2008 (complexes) | Pfam domains | YeastRC Public Image Repository |
| Candida (CandidaDB) | Complexome | YeastRC Structure Prediction (Seattle) | |
| YGOB | DIP | ||
| YOGY | GeneMANIA |
References cited on this page View Complete Literature Guide for Vps21p
| 1) | Robinson JS, et al. (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48 |
| 2) | Rothman JH, et al. (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65 |
| 3) | Raymond CK, et al. (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402 |
| 4) | Horazdovsky BF, et al. (1994) VPS21 encodes a rab5-like GTP binding protein that is required for the sorting of yeast vacuolar proteins. EMBO J 13(6):1297-309 |
| 5) | Singer-Kruger B, et al. (1994) Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast. J Cell Biol 125(2):283-98 |
| 6) | Neukamm B, et al. (2002) Endocytosis is involved in DNA uptake in yeast. Biochim Biophys Acta 1572(1):67-76 |
| 7) | Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61 |
| 8) | Nickerson DP, et al. (2012) Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3. Traffic 13(10):1411-28 |
| 9) | Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76 |
| 10) | Cabrera M, et al. (2013) Functional separation of endosomal fusion factors and the class C core vacuole/endosome tethering (CORVET) complex in endosome biogenesis. J Biol Chem 288(7):5166-75 |
| 11) | Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41 |
