SKI7/YOR076C Protein Information Help

Standard Name Ski7p 1
Systematic Name Yor076cp
ORF Classification Verified
Description Coupling protein for the Ski complex and cytoplasmic exosome; involved in 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype; SKI7 has a paralog, HBS1, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7)
Name Description SuperKIller 1
Experimental Data
Molecules/cell 233 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 747
Molecular Weight (Da) 84,778
Isoelectric Point (pI) 7.77

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ski7p (InterPro)
Physical Interactions There are 80 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052F3F | Q08491
MIPS: YOR076C
NCBI: 1420233 | 2104888 | 6324650 | 74676474 | NP_014719.1 | NM_001183495.1
GenBank/EMBL/DDBJ: DAA10855.1 | Z70678 | Z74984
Amino Acid Sequence (or in FASTA format)
 
       1  MSLLEQLARK RIEKSKGLLS ADQSHSTSKS ASLLERLHKN RETKDNNAET
      51  KRKDLKTLLA KDKVKRSDFT PNQHSVSLSL KLSALKKSNS DLEKQGKSVT
     101  LDSKENELPT KRKSPDDKLN LEESWKAIKE MNHYCFLKND PCINQTDDFA
     151  FTNFIIKDKK NSLSTSIPLS SQNSSFLSLK KHNNELLGIF VPCNLPKTTR
     201  KVAIENFNRP SPDDIIQSAQ LNAFNEKLEN LNIKSVPKAE KKEPINLQTP
     251  PTESIDIHSF IATHPLNLTC LFLGDTNAGK STLLGHLLYD LNEISMSSMR
     301  ELQKKSSNLD PSSSNSFKVI LDNTKTEREN GFSMFKKVIQ VENDLLPPSS
     351  TLTLIDTPGS IKYFNKETLN SILTFDPEVY VLVIDCNYDS WEKSLDGPNN
     401  QIYEILKVIS YLNKNSACKK HLIILLNKAD LISWDKHRLE MIQSELNYVL
     451  KENFQWTDAE FQFIPCSGLL GSNLNKTENI TKSKYKSEFD SINYVPEWYE
     501  GPTFFSQLYL LVEHNMNKIE TTLEEPFVGT ILQSSVLQPI AEINYVSLKV
     551  LINSGYIQSG QTIEIHTQYE DFHYYGIVSR MKNSKQILET NTKNNISVGL
     601  NPDILEVLVK IHNTEDFTKK QFHIRKGDII IHSRKTNTLS PNLPNTLKLL
     651  ALRLIKLSIQ THALSDPVDL GSELLLYHNL THNAVKLVKI LGTNDISINP
     701  NQSLIVEVEI IEPDFALNVI DSKYITNNIV LTSIDHKVIA VGRIACQ*

external links for Ski7p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ski7p
1) Ridley SP, et al.  (1984) Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN. Mol Cell Biol 4(4):761-70
2) Benard L, et al.  (1999) The ski7 antiviral protein is an EF1-alpha homolog that blocks expression of non-Poly(A) mRNA in Saccharomyces cerevisiae. J Virol 73(4):2893-900
3) van Hoof A, et al.  (2000) Function of the ski4p (Csl4p) and Ski7p proteins in 3'-to-5' degradation of mRNA. Mol Cell Biol 20(21):8230-43
4) Araki Y, et al.  (2001) Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast. EMBO J 20(17):4684-93
5) van Hoof A, et al.  (2002) Exosome-mediated recognition and degradation of mRNAs lacking a termination codon. Science 295(5563):2262-4
6) Wang L, et al.  (2005) Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p. RNA 11(8):1291-302
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41