VPS5/YOR069W Protein Information Help

Standard Name Vps5p 1, 2
Systematic Name Yor069wp
Alias Grd2p , Pep10p , Vpt5p 3
ORF Classification Verified
Description Nexin-1 homolog; required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to late Golgi; structural component of retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes; VPS5 has a paralog, YKR078W, that arose from the whole genome duplication (4, 5, 6, 7, 8) Also known as: YOR29-20
Name Description Vacuolar Protein Sorting 1, 2
Experimental Data
Molecules/cell 6120 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 675
Molecular Weight (Da) 76,484
Isoelectric Point (pI) 4.93

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Vps5p (InterPro)
Physical Interactions There are 71 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052FC1 | Q92331
MIPS: YOR069W
NCBI: 1420219 | 1657952 | 1814080 | 2104881 | 2499128 | 45269960 | 46562125 | NP_014712.2 | NM_001183488.1
GenBank/EMBL/DDBJ: AAB41798.1 | AAB62976.1 | AAS56361.1 | CAA94554.1 | CAA99262.1 | DAA10847.1 | AY558035 | U73512 | U84735 | Z70678 | Z74976 | Z74977
Amino Acid Sequence (or in FASTA format)
       1  MDYEDNLEAP VWDELNHEGD KTQSLVSNSI ESIGQISTNE ERKDNELLET
      51  TASFADKIDL DSAPEWKDPG LSVAGNPQLE EHDNSKADDL INSLAPEQDP
     101  IADLKNSTTQ FIATRESGGA LFTGNANSPL VFDDTIYDAN TSPNTSKSIS
     151  GRRSGKPRIL FDSARAQRNS KRNHSLKAKR TTASDDTIKT PFTDPLKKAE
     201  KENEFVEEPL DDRNERRENN EGKFTASVEK NILEQVDRPL YNLPQTGANI
     251  SSPAEVEENS EKFGKTKIGS KVPPTEKAVA FKVEVKDPVK VGELTSIHVE
     301  YTVISESSLL ELKYAQVSRR YRDFRWLYRQ LQNNHWGKVI PPPPEKQSVG
     351  SFKENFIENR RFQMESMLKK ICQDPVLQKD KDFLLFLTSD DFSSESKKRA
     401  FLTGSGAIND SNDLSEVRIS EIQLLGAEDA AEVLKNGGID AESHKGFMSI
     451  SFSSLPKYNE ADEFFIEKKQ KIDELEDNLK KLSKSLEMVD TSRNTLAAST
     501  EEFSSMVETL ASLNVSEPNS ELLNNFADVH KSIKSSLERS SLQETLTMGV
     551  MLDDYIRSLA SVKAIFNQRS KLGYFLVVIE NDMNKKHSQL GKLGQNIHSE
     601  KFREMRKEFQ TLERRYNLTK KQWQAVGDKI KDEFQGFSTD KIREFRNGME
     651  ISLEAAIESQ KECIELWETF YQTNL*                          

external links for Vps5p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) CYC2008 (complexes) MIPS YeastGFP
Candida (CGD) Complexome Pfam domains
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA


IMP


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Vps5p
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Bankaitis VA, et al.  (1986) Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A 83(23):9075-9
4) Seaman MN, et al.  (1998) A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast. J Cell Biol 142(3):665-81
5) Nothwehr SF and Hindes AE  (1997) The yeast VPS5/GRD2 gene encodes a sorting nexin-1-like protein required for localizing membrane proteins to the late Golgi. J Cell Sci 110 ( Pt 9)():1063-72
6) Reddy JV and Seaman MN  (2001) Vps26p, a component of retromer, directs the interactions of Vps35p in endosome-to-Golgi retrieval. Mol Biol Cell 12(10):3242-56
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Liu TT, et al.  (2012) Rab GTPase regulation of retromer-mediated cargo export during endosome maturation. Mol Biol Cell 23(13):2505-15
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41